Recombinant Human ABRA 293 Cell Lysate

• Cat.No.: ABRA-9121HCL

Specification

• Description: Antigen standard for actin-binding Rho activating protein (ABRA) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ABRA actin-binding Rho activating protein [ Homo sapiens ]
• Official Symbol: ABRA
• Synonyms: ABRA; actin-binding Rho activating protein; actin-binding Rho-activating protein; STARS; striated muscle activator of Rho dependent signaling; striated muscle activator of Rho-dependent signaling
• Gene ID: 137735
• mRNA Refseq: NM_139166
• Protein Refseq: NP_631905
• MIM: 609747
• UniProt ID: Q8N0Z2
• Chromosome Location: 8q23.1
• Function: actin binding; transcription coactivator activity

Reviews

11/07/2022

Fast shipping, cheap price, good quality product.

08/04/2022

There was a shipment according to my request and the product was received in lyophilized powder form.

08/14/2020

I purchased protein for western blotting and it works well, the bands are very clear.

01/28/2019

Custom-made protein at a good price and good quality.

Q&As

Where is the ABRA protein located?

ABRA is specifically localized on the Z disc and M-line.

Do you provide ABRA protein sourced from E.coli?

We can provide ABRA protein sourced from E.coli.

Is it possible to customize His-tagged ABRA protein?

Yes, we can custom produce His-tagged ABRA protein for you.

Is the purity of ABRA protein high?

We currently offer ABRA proteins with purity > 80%, if you have a higher purity requirement you can talk to our experts.

Can the endotoxin in ABRA be removed?

We can remove endotoxins according to your needs.

Do you offer ABRA produced with other protein expression systems?

Yes, we can custom produce ABRA according to your needs.

How effective is ABRA for western blotting? Are the bands clear?

Our customer purchased ABRA for western blotting and obtained clear bands.

Do you offer ABRA protein that can be used in WB?

We have ABRA available for western blotting.

Is ABRA a lyophilized powder or a liquid?

We will ship according to stock, or if you have a request for this, you can specify it with us in advance.

Can ABRA be customized?

Yes, you can communicate with us directly for customization requirements.