Recombinant Human ACACB protein, His & T7-tagged

• Cat.No.: ACACB-8156H
• Product Overview: Recombinant Human ACACB aa. (Ser29~Glu249 (Accession # O00763)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 27.2kDa.
• Protein length: Ser29~Glu249 (Accession # O00763)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 9.3.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: ACACB acetyl-CoA carboxylase beta [ Homo sapiens (human) ]
• Official Symbol: ACACB
• Synonyms: ACACB; acetyl-CoA carboxylase beta; ACC2; ACCB; HACC275; acetyl-CoA carboxylase 2; ACC-beta; acetyl-Coenzyme A carboxylase beta
• Gene ID: 32
• mRNA Refseq: NM_001093.3
• Protein Refseq: NP_001084.3
• UniProt ID: O00763

Reviews

09/05/2022

Demonstrates high selectivity and sensitivity in detecting protein-protein interactions in pull-down assays.

02/18/2018

Provides accurate and reliable quantification of protein biomarkers in clinical samples.

Q&As

What are the downstream targets of acetyl-CoA carboxylase beta protein in lipid synthesis?

The downstream targets of acetyl-CoA carboxylase beta protein include fatty acid synthase and other enzymes involved in lipid synthesis.

What is the role of acetyl-CoA carboxylase beta protein in lipid metabolism?

Acetyl-CoA carboxylase beta protein is responsible for the carboxylation of acetyl-CoA, which is a key step in fatty acid synthesis.

How does acetyl-CoA carboxylase beta protein contribute to insulin resistance and metabolic diseases?

Acetyl-CoA carboxylase beta protein has been implicated in the development of insulin resistance and metabolic diseases due to its role in lipid metabolism.

How is acetyl-CoA carboxylase beta protein regulated in the cell?

Acetyl-CoA carboxylase beta protein is regulated by various post-translational modifications and allosteric regulators.

What is the structure of acetyl-CoA carboxylase beta protein?

Acetyl-CoA carboxylase beta protein is a multi-domain protein composed of several structural domains.

How does acetyl-CoA carboxylase beta protein interact with other proteins in lipid metabolism pathways?

Acetyl-CoA carboxylase beta protein interacts with several other proteins involved in lipid metabolism, including fatty acid synthase and AMP-activated protein kinase.

What is the function of acetyl-CoA carboxylase beta protein in the body?

Acetyl-CoA carboxylase beta protein plays a crucial role in fatty acid metabolism and lipid synthesis.

What is the expression pattern of acetyl-CoA carboxylase beta protein in different tissues?

Acetyl-CoA carboxylase beta protein is expressed in various tissues, with the highest expression observed in the liver and adipose tissue.

What are the genetic variants associated with acetyl-CoA carboxylase beta protein and metabolic disorders?

Several genetic variants in the acetyl-CoA carboxylase beta gene have been associated with metabolic disorders such as obesity and type 2 diabetes.

What are the potential therapeutic targets for regulating acetyl-CoA carboxylase beta protein activity?

Potential therapeutic targets for regulating acetyl-CoA carboxylase beta protein activity include small molecule inhibitors and modulators of its regulatory pathways.