Recombinant Human ACAD9 293 Cell Lysate

• Cat.No.: ACAD9-9115HCL

Specification

• Description: Antigen standard for acyl-Coenzyme A dehydrogenase family, member 9 (ACAD9) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ACAD9 acyl-CoA dehydrogenase family, member 9 [ Homo sapiens ]
• Official Symbol: ACAD9
• Synonyms: ACAD9; acyl-CoA dehydrogenase family, member 9; acyl Coenzyme A dehydrogenase family, member 9; acyl-CoA dehydrogenase family member 9, mitochondrial; MGC14452; NPD002; very-long-chain acyl-CoA dehydrogenase VLCAD; acyl-Coenzyme A dehydrogenase family, member 9; FLJ23533
• Gene ID: 28976
• mRNA Refseq: NM_014049
• Protein Refseq: NP_054768
• MIM: 611103
• UniProt ID: Q9H845
• Chromosome Location: 3q21.3
• Function: acyl-CoA dehydrogenase activity; flavin adenine dinucleotide binding

Reviews

12/23/2019

Provides high-quality results in detecting and measuring the activity of oxidoreductases in oxidoreductase assays.

02/15/2018

Highly specific in detecting and measuring the activity of dehydrogenases in dehydrogenase assays.

Q&As

What is the structure of this protein and how does it relate to its function?

The protein structure of acyl-CoA dehydrogenase family member 9 includes a flavin adenine dinucleotide (FAD) binding domain.

What diseases or disorders are associated with mutations in this protein?

Mutations in this protein are associated with various metabolic disorders, including mitochondrial fatty acid oxidation disorders.

What are the physiological roles of acyl-CoA dehydrogenase family member 9?

The physiological roles of acyl-CoA dehydrogenase family member 9 are still being investigated.

What is the primary function of acyl-CoA dehydrogenase family member 9 protein?

The primary function of acyl-CoA dehydrogenase family member 9 protein is currently unknown.

Are there any known inhibitors or activators of this protein?

There are currently no known inhibitors or activators of this protein.

What research is currently being conducted to further understand the function of acyl-CoA dehydrogenase family member 9?

Research is ongoing to further understand the function and role of acyl-CoA dehydrogenase family member 9 in cellular metabolism and disease.

How does the activity of this protein contribute to cellular metabolism?

The activity of this protein contributes to fatty acid metabolism and energy production.

How is the expression of acyl-CoA dehydrogenase family member 9 regulated?

The expression of acyl-CoA dehydrogenase family member 9 is regulated by a variety of factors, including gene expression and protein degradation pathways.

What is the cellular location of this protein within the cell?

The cellular location of this protein within the cell is the mitochondria.

What are the substrates that can be acted upon by acyl-CoA dehydrogenase family member 9?

Acyl-CoA dehydrogenase family member 9 can act on medium to long-chain fatty acids.