Recombinant Human ACADVL, His-tagged

• Cat.No.: ACADVL-26050TH
• Product Overview: Recombinant full length Human ACADVL with an N terminal His tag; 636 amino acids including tag, MWt 68.5kDa.

Specification

• Description: The protein encoded by this gene is targeted to the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenase is specific to long-chain and very-long-chain fatty acids. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Protein length: 615 amino acids
• Conjugation: HIS
• Molecular Weight: 68.500kDa inclusive of tags
• Source: E. coli
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM EDTA, 1mM DTT, pH 8.0
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMAGGA AQLALDKSDSHPSDALTRKKPAKAE SKSFAVGMFKGQLTTDQVFPYPSVL NEEQTQFLKELVEPVSRFFEEVNDP AKNDALEMVEETTWQGLKELGAFGL QVPSELGGVGLCNTQYARLVEIVGM HDLGVGITLGAHQSIGFKGILLFGT KAQKEKYLPKLASGETVAAFCLTEP SSGSDAASIRTSAVPSPCGKYYTLN GSKLWISNGGLADIFTVFAKTPVTD PATGAVKEKITAFVVERGFGGITHG PPEKKMGIKASNTAEVFFDGVRVPS ENVLGEVGSGFKVAMHILNNGRFGM AAALAGTMRGIIAKAVDHATNRTQF GEKIHNFGLIQEKLARMVMLQYVTE SMAYMVSANMDQGATDFQIEAAISK IFGSEAAWKVTDECIQIMGGMGFMK EPGVERVLRDLRIFRIFEGTNDILR LFVALQGCMDKGKELSGLGSALKNP FGNAGLLLGEAGKQLRRRAGLGSGL SLSGLVHPELSRSGELAVRALEQFA TVVEAKLIKHKKGIVNEQFLLQRLA DGAIDLYAMVVVLSRASRSLSEGHP TAQHEKMLCDTWCIEAAARIREGMA ALQSDPWQQELYRNFKSISKALVER GGVVTSNPLGF
• Sequence Similarities: Belongs to the acyl-CoA dehydrogenase family.

Gene Information

• Gene Name: ACADVL acyl-CoA dehydrogenase, very long chain [ Homo sapiens ]
• Official Symbol: ACADVL
• Synonyms: ACADVL; acyl-CoA dehydrogenase, very long chain; acyl Coenzyme A dehydrogenase, very long chain; very long-chain specific acyl-CoA dehydrogenase, mitochondrial; ACAD6; LCACD; VLCAD
• Gene ID: 37
• mRNA Refseq: NM_000018
• Protein Refseq: NP_000009
• MIM: 609575
• Uniprot ID: P49748
• Chromosome Location: 17p13.1
• Pathway: Activation of Chaperones by IRE1alpha, organism-specific biosystem; Beta oxidation of palmitoyl-CoA to myristoyl-CoA, organism-specific biosystem; Diabetes pathways, organism-specific biosystem; FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem; Fatty Acid Beta Oxidation, organism-specific biosystem
• Function: acyl-CoA dehydrogenase activity; fatty-acyl-CoA binding; flavin adenine dinucleotide binding; long-chain-acyl-CoA dehydrogenase activity; very long-chain-acyl-CoA dehydrogenase activity

Reviews

09/15/2022

I was very impressed with the customized recombinant protein that Creative BioMart created for me. The technical support experts were extremely helpful and accommodating, and the product exceeded my expectations.

05/08/2021

I have been consistently impressed with the quality and purity of Creative BioMart's recombinant ACADVL proteins. Their products are reliable and cost-effective, and I plan to repurchase from them in the future.

01/16/2020

Thanks to Creative BioMart's ACADVL recombinant protein, I was able to save a significant amount of time on my project. The product was of high quality and reasonably priced.

Q&As

What are the applications of the recombinant ACADVL protein?

The recombinant ACADVL protein can be used in various applications, such as enzyme assays, drug screening, and protein-protein interaction studies.

What are the recommended usage guidelines of your recombinant ACADVL protein?

The recommended usage guidelines can vary depending on the specific application, but typically involve optimizing the protein concentration, buffer conditions, and other experimental parameters.

Can your recombinant ACADVL protein be used in biomarker research?

Sure, ACADVL has been suggested as a potential biomarker for various diseases, including non-alcoholic fatty liver disease (NAFLD) and type 2 diabetes.

What diseases can be caused by abnormal expression of ACADVL?

ACADVL deficiency reduces myocardial fatty acid β-oxidation and is associated with cardiomyopathy. In addition, very long-chain acyl-CoA dehydrogenase deficiency is a rare, life-threatening disorder of mitochondrial fatty acid oxidation metabolism caused by mutations in the ACADVL gene.

How is ACADVL implicated in disease, and what are some potential therapeutic targets?

Mutations in the ACADVL gene can lead to VLCADD, a rare inherited disorder that affects the metabolism of VLCFAs. Symptoms of VLCADD can include muscle weakness, lethargy, and hypoglycemia. A recent study investigated the impact of ACADVL deficiency on mitochondrial function and found that it leads to impaired fatty acid oxidation and increased oxidative stress.

What quality control measures are in place to ensure the recombinant ACADVL protein is of high quality?

These quality control measures can include testing for purity, concentration, and functionality, as well as ensuring the protein is free from contaminants, such as endotoxins or microbial contaminants.

Can you customize recombinant proteins with potential hotspot ACADVL mutations?

Sure, you can describe the specific mutation site in the online form, and our scientists will tailor the corresponding protein products for you.

What is the role of ACADVL in lipid metabolism?

ACADVL has been implicated in several lipid metabolic pathways, including the beta-oxidation of fatty acids, the biosynthesis of unsaturated fatty acids, and the synthesis of ether lipids.

Do you have commercial antibodies for ACADVL?

You can submit specific requirements, and our experts will contact you.

Can your recombinant ACADVL protein be used for structural studies?

Our high-purity recombinant proteins are suitable for biophysics-related research, such as structural elucidation, molecular dynamics simulations, and interaction studies.