Recombinant Human ACBD3 293 Cell Lysate

• Cat.No.: ACBD3-9107HCL

Specification

• Description: Antigen standard for acyl-Coenzyme A binding domain containing 3 (ACBD3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ACBD3 acyl-CoA binding domain containing 3 [ Homo sapiens ]
• Official Symbol: ACBD3
• Synonyms: ACBD3; acyl-CoA binding domain containing 3; acyl Coenzyme A binding domain containing 3 , GOCAP1, golgi complex associated protein 1, 60kDa , GOLPH1; Golgi resident protein GCP60; GCP60; PAP7; PBR and PKA associated protein 7; golgi phosphoprotein 1; PKA (RIalpha)-associated protein; PBR- and PKA-associated protein 7; golgi complex associated protein 1, 60kDa; acyl-Coenzyme A binding domain containing 3; peripheral benzodiazepine receptor-associated protein PAP7; GOCAP1; GOLPH1
• Gene ID: 64746
• mRNA Refseq: NM_022735
• Protein Refseq: NP_073572
• MIM: 606809
• UniProt ID: Q9H3P7
• Chromosome Location: 1q41
• Function: binding; fatty-acyl-CoA binding

Reviews

09/05/2021

I purchased the recombinant Rat ACBD3 protein from Creative BioMart and was impressed by their fast delivery and great customer service.

06/18/2021

The technical support team at Creative BioMart was very helpful in assisting me with selecting the right fusion tag for the recombinant human ACBD3 protein.

08/01/2019

In our experiments, we utilized the Recombinant Human ACBD3 Protein. This exceptional protein performed flawlessly in our evaluations, demonstrating the high caliber of Creative BioMart's products.

Q&As

What are the downstream targets of ACBD3 in lipid metabolism?

The downstream targets of ACBD3 in lipid metabolism include various enzymes involved in lipid metabolism such as acyl-CoA synthetases, acyl-CoA dehydrogenases, and acyltransferases.

What is the recommended method for determining the protein concentration of recombinant ACBD3 protein?

Our recombinant protein products have been tested for concentration. Of course, you can also determine the concentration again in experiments such as spectrophotometry, BCA assay, or Bradford assay.

What assays can be performed using your recombinant ACBD3 protein?

Our Recombinant ACBD3 Protein can be used in a number of assays, such as fluorescence resonance energy transfer (FRET) assays, and other protein-protein interaction assays.

I plan to buy Recombinant Chicken ACBD3 (ACBD3-2723C), is there any discount?

Discounts are available for first-time purchases and holidays. For specific discounts, please contact our customer service staff.

How is ACBD3 involved in vesicle transport and protein trafficking?

ACBD3 regulates vesicle transport and protein trafficking by interacting with various proteins involved in these processes and helping to localize them to the appropriate cellular compartments.

What is the recommended method for detecting ACBD3 in cells and tissues?

It may involve Western blotting using an ACBD3-specific antibody.

Can the Recombinant ACBD3 Protein be used for in vitro assays or only for in vivo studies?

The Recombinant ACBD3 Protein can be used for both in vitro and in vivo studies, depending on the experimental design.

Can the Recombinant ACBD3 Protein be used for crystallization studies?

Yes, our recombinant ACBD3 protein may be suitable for crystallization studies or other structural biology applications, but this will depend on the specific protein fragment, preparation, and experimental conditions.

What is the role of ACBD3 in the Golgi apparatus and endoplasmic reticulum?

ACBD3 is involved in regulating vesicle transport and protein trafficking in the Golgi apparatus and endoplasmic reticulum by helping to localize various proteins involved in these processes.

What is the recommended concentration of the Recombinant Human ACBD3 Protein (ACBD3-146H) for experiments?

The recommended concentration of this protein may depend on your specific experiment, but a starting concentration of 0.1-1 mg/mL is generally recommended.