Recombinant Human Acetyl-CoA Acyltransferase 1, His-tagged

• Cat.No.: ACAA1-784H
• Product Overview: Recombinant humanACAA1 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 43.8 kDa (419aa)confirmed by MALDI-TOF.

Specification

• Cat. No.: ACAA1-784H
• Description: ACAA1 is a member of the thiolase family of enzymes and is involved in lipid metabolism. This protein is localized to the peroxisome and catalyzes the conversion of acyl-CoA and acetyl-CoA to 3-oxoacyl-CoA in the fatty acid oxidation pathway. ACAA1 shows high enzymatic activity in liver, kidney, intestine and white adipose tissue in rats. Deficiency of this enzyme leads to pseudo-Zellweger syndrome.
• Source: E.coli.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MLSGAPQASA ADVVVVHGRR TAICRAGRGG FKDTTPDELL SAVMTAVLKD VNLRPEQLGD ICVGNVLQPG AGAIMARIAQ FLSDIPETVP LSTVNRQCSS GLQAVASIAG GIRNGSYDIG MACGVESMSL ADRGNPGNIT SRLMEKEKAR DCLIPMGITS ENVAERFGIS REKQDTFALA SQQKAARAQS KGCFQAEIVP VTTTVHDDKG TKRSITVTQD EGIRPSTTME GLAKLKPAFK KDGSTTAGNS SQVSDGAAAI LLARRSKAEE LGLPILGVLR SYAVVGVPPD IMGIGPAYAI PVALQKAGLT VSDVDIFEIN EAFASQAAYC VEKLRLPPEK VNPLGGAVAL GHPLGCTGAR QVITLLNELK RRGKRAYGVV SMCIGTGMGA AAVFEYPGN
• Purity: > 95% by SDS – PAGE.
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol 1mM DTT, 0.1M NaCl.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: ACAA1 acetyl-CoA acyltransferase 1 [ Homo sapiens ]
• Synonyms: ACAA1; acetyl-CoA acyltransferase 1; ACAA; THIO; PTHIO; 3-ketoacyl-CoA thiolase, peroxisomal; beta-ketothiolase; OTTHUMP00000209180; OTTHUMP00000209821; OTTHUMP00000209822; peroxisomal 3-oxoacyl-CoA thiolase; acetyl-Coenzyme A acyltransferase 1; peroxisomal 3-oxoacyl-Coenzyme A thiolase; EC 2.3.1.16
• Gene ID: 30
• mRNA Refseq: NM_001130410
• Protein Refseq: NP_001123882
• MIM: 604054
• UniProt ID: P09110
• Chromosome Location: 3p23-p22
• Pathway: Biosynthesis of unsaturated fatty acids; Fatty acid metabolism; Metabolic pathways; PPAR signaling pathway; KEGG pathway; Valine, leucine and isoleucine degradation
• Function: acetyl-CoA C-acyltransferase activity; acyltransferase activity; protein binding; transferase activity

Reviews

04/22/2022

Provides high signal-to-noise ratio and low background in detection of the target protein in various assays.

11/09/2021

Highly effective in blocking the activity of the target protein in functional studies.

Q&As

What are the potential therapeutic applications of modulating acetyl-CoA acyltransferase 1 protein activity?

Modulating its activity could potentially treat metabolic disorders or enhance ketone body production.

What is the structure of acetyl-CoA acyltransferase 1 protein?

It is a dimeric protein with a helix-turn-helix motif.

What is the role of acetyl-CoA acyltransferase 1 protein in metabolism?

It plays a crucial role in beta-oxidation of fatty acids and ketone body synthesis.

What is the function of acetyl-CoA acyltransferase 1 protein?

It catalyzes the transfer of an acyl group from acyl-CoA to CoA.

How does acetyl-CoA acyltransferase 1 protein interact with other proteins or enzymes?

It interacts with other enzymes in the beta-oxidation pathway, including acyl-CoA dehydrogenases and enoyl-CoA hydratases.

How is the expression of acetyl-CoA acyltransferase 1 protein regulated?

Its expression is regulated by transcription factors, hormones, and metabolic signals.

What are the biochemical pathways that involve acetyl-CoA acyltransferase 1 protein?

It is involved in the metabolism of long-chain fatty acids, branched-chain amino acids, and isoprenoids.

What are the physiological consequences of acetyl-CoA acyltransferase 1 protein deficiency or overexpression?

Deficiency can lead to metabolic disorders, such as hypoketotic hypoglycemia, while overexpression may increase ketone body production.

Where is acetyl-CoA acyltransferase 1 protein expressed in the body?

It is expressed in various tissues, including liver, adipose tissue, and muscle.

How does acetyl-CoA acyltransferase 1 protein contribute to the pathogenesis of metabolic diseases?

Dysregulation of its activity may contribute to the development of metabolic diseases such as diabetes and obesity.