Recombinant Human ACO1 protein, His-tagged

• Cat.No.: ACO1-3259H
• Product Overview: Recombinant Human ACO1 protein(540-890 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 540-890 aa
• AA Sequence: GRVHPNTRANYLASPPLVIAYAIAG TIRIDFEKEPLGVNAKGQQVFLKDI WPTRDEIQAVERQYVIPGMFKEVYQ KIETVNESWNALATPSDKLFFWNSK STYIKSPPFFENLTLDLQPPKSIVD AYVLLNLGDSVTTDHISPAGNIARN SPAARYLTNRGLTPREFNSYGSRRG NDAVMARGTFANIRLLNRFLNKQAP QTIHLPSGEILDVFDAAERYQQAGL PLIVLAGKEYGAGSSRDWAAKGPFL LGIKAVLAESYERIHRSNLVGMGVI PLEYLPGENADALGLTGQERYTIII PENLKPQMKVQVKLDTGKTFQAVMR FDTDVELTYFLNGGILNYMIRKMAK
• Purity: 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: ACO1 aconitase 1, soluble [ Homo sapiens ]
• Official Symbol: ACO1
• Synonyms: ACO1; aconitase 1, soluble; IREB1; cytoplasmic aconitate hydratase; IREBP; IRP1; IRE-BP 1; aconitate hydratase; citrate hydro-lyase; iron regulatory protein 1; ferritin repressor protein; iron-responsive element binding protein 1; iron-responsive element-binding protein 1; ACONS; IREBP1
• Gene ID: 48
• mRNA Refseq: NM_002197
• Protein Refseq: NP_002188
• MIM: 100880
• UniProt ID: P21399