Recombinant Human ACO2, GST-tagged
Cat.No. : | ACO2-9295H |
Product Overview : | Recombinant Human ACO2 protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose. |
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Description : | The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. |
Source : | E.coli |
Species : | Human |
Tag : | GST |
Protein length : | N-term-300a.a. |
Storage : | The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles. |
Storage Buffer : | 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol. |
Gene Name : | ACO2 aconitase 2, mitochondrial [ Homo sapiens ] |
Official Symbol : | ACO2 |
Synonyms : | ACO2; aconitase 2, mitochondrial; aconitate hydratase, mitochondrial; ACONM; citrate hydro-lyase; ICRD; MGC20605; MGC33908; |
Gene ID : | 50 |
mRNA Refseq : | NM_001098 |
Protein Refseq : | NP_001089 |
MIM : | 100850 |
UniProt ID : | Q99798 |
Chromosome Location : | 22q13.2 |
Pathway : | Citrate cycle (TCA cycle), organism-specific biosystem; Citrate cycle (TCA cycle), conserved biosystem; Citrate cycle, first carbon oxidation, oxaloacetate => 2-oxoglutarate, organism-specific biosystem; Citrate cycle, first carbon oxidation, oxaloacetate => 2-oxoglutarate, conserved biosystem; Citric acid cycle (TCA cycle), organism-specific biosystem; |
Function : | 3 iron, 4 sulfur cluster binding; 4 iron, 4 sulfur cluster binding; aconitate hydratase activity; citrate hydro-lyase (cis-aconitate-forming) activity; iron ion binding; isocitrate hydro-lyase (cis-aconitate-forming) activity; lyase activity; metal ion bi |
Products Types
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ACO2-2490H | Recombinant Human ACO2 protein(Gln28-Gln780), His&GST-tagged | +Inquiry |
ACO2-111R | Recombinant Rat ACO2 Protein, His (Fc)-Avi-tagged | +Inquiry |
ACO2-914H | Recombinant Human ACO2 Protein, MYC/DDK-tagged | +Inquiry |
ACO2-38R | Recombinant Rhesus Macaque ACO2 Protein, His (Fc)-Avi-tagged | +Inquiry |
Aco2-513M | Recombinant Mouse Aco2 Protein, MYC/DDK-tagged | +Inquiry |
◆ Lysates | ||
ACO2-498HCL | Recombinant Human ACO2 cell lysate | +Inquiry |
ACO2-440MCL | Recombinant Mouse ACO2 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionACO2 is involved in the TCA cycle, which is a critical process for energy production in cells. Specifically, it catalyzes the conversion of citrate to isocitrate, which is a crucial step in the cycle.
Yes, mutations in the ACO2 gene have been associated with several disorders, including infantile cerebellar-retinal degeneration and mitochondrial encephalopathy. These conditions are characterized by a range of symptoms, including neurological and developmental abnormalities.
ACO2 is a potential target for cancer treatment, as it is overexpressed in several types of cancer cells. However, the development of drugs that target ACO2 is still in the early stages of research.
ACO1 and ACO2 are two isoforms of aconitate hydratase, which catalyze the same reaction in the TCA cycle. However, ACO2 is primarily located in the mitochondria of cells, while ACO1 is located in the cytoplasm.
Unlike ACO1, ACO2 is not involved in iron metabolism. Its role is primarily in energy production through the TCA cycle.
ACO2 deficiency has been associated with several disorders, including infantile cerebellar-retinal degeneration and mitochondrial encephalopathy. These conditions are characterized by various neurological and developmental abnormalities.
ACO2 is a homodimeric enzyme composed of two identical subunits. Each subunit contains a catalytic domain and a regulatory domain, which work together to catalyze the isomerization of citrate to isocitrate.
ACO2 activity can be measured in the laboratory using a variety of techniques, including spectrophotometric assays and enzymatic activity assays. These assays typically involve measuring the rate of citrate isomerization to isocitrate in the presence of ACO2 and other necessary cofactors.
ACO2 activity is regulated by a variety of factors, including substrate availability, pH, and post-translational modifications such as phosphorylation. Additionally, ACO2 activity can be modulated by other enzymes and metabolic pathways in the cell.
While ACO2 is primarily known for its role in energy production, recent research suggests that it may also play a role in other cellular processes. For example, studies have shown that ACO2 may be involved in regulating the immune response and cellular metabolism.
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