Recombinant Human ACP3 Full Length Transmembrane protein, His-tagged

• Cat.No.: ACP3-9284H
• Product Overview: Recombinant Human ACP3 protein(P15309)(33-418aa), fused with N-terminal His tag(This tag can be tested only under denaturing conditions), was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
  If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 47.5 kDa
• Protein Length: 33-418aa
• AA Sequence: KELKFVTLVFRHGDRSPIDTFPTDP IKESSWPQGFGQLTQLGMEQHYELG EYIRKRYRKFLNESYKHEQVYIRST DVDRTLMSAMTNLAALFPPEGVSIW NPILLWQPIPVHTVPLSEDQLLYLP FRNCPRFQELESETLKSEEFQKRLH PYKDFIATLGKLSGLHGQDLFGIWS KVYDPLYCESVHNFTLPSWATEDTM TKLRELSELSLLSLYGIHKQKEKSR LQGGVLVNEILNHMKRATQIPSYKK LIMYSAHDTTVSGLQMALDVYNGLL PPYASCHLTELYFEKGEYFVEMYYR NETQHEPYPLMLPGCSPSCPLERFA ELVGPVIPQDWSTECMTTNSHQVLK VIFAVAFCLISAVLMVLLFIHIRRG LCWQRESYGNI
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C.

Reviews

01/19/2021

It worked very beautifully!

01/15/2020

Very pleased with the results, I will recommended it to my colleagues :)

07/03/2019

This product could deonstrate a clear image at appliable lab instruments :)

06/03/2019

You are so fast with delivery!

05/23/2019

A wonderful lab product with detailed instructions.

Q&As

What is the expression of ACP3 in tissue spectificity?

Highly expressed in the prostate, restricted to glandular and ductal epithelial cells. Also expressed in bladder, kidney, pancreas, lung, cervix, testis and ovary. Weak expression in a subset of pancreatic islet cells, squamous epithelia, the pilosebaceous unit, colonic neuroendocrine cells and skin adnexal structures. Low expression in prostate carcinoma cells and tissues

What condition does ACP3 need for dephosphorylation when it acts as enzyme?

The dephosphorylation can produce a diverse number of substrates under acidic conditions (pH 4-6)

What disease associated with the ACP3?

Diseases associated with ACP3 include prostatitis and clear cell adenocarcinoma.

What is the activity regulation of ACP3?

Phosphatase activity inhibited by L+-tartrate, and by its derivative, alpha-benzylaminobenzylphosphonic acid.

What are suggested Antigen Peptide Sequences for ACP3 gene?

ACPP protein, thiamine monophosphatase, etc.

What is the function of the isoform2 for ACP3?

Tyrosine phosphatase that acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling 

Could please generally describe the function of ACP3?

ACP3 has plenty of functions, inlcuding cancer-related genes Enzymes; FDA approved drug targets; Metabolic proteins; Predicted intracellular proteins, etc.

What is the quaternary structure of ACP3 protein?

Homodimer; dimer formation is required for phosphatase activity.

Could please list three bioligical processes of ACP3?

Adenosine metabolic process; Positive regulation of adenosine receptor signaling pathway; Sensory perception of pain

What is the buffer solution of the ACP3?

Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose.