Recombinant Human ACPL2 cell lysate

• Cat.No.: ACPL2-2290HCL
• Product Overview: Human ACPL2 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: ACPL2 acid phosphatase-like 2 [ Homo sapiens ]
• Official Symbol: ACPL2
• Synonyms: ACPL2; acid phosphatase-like 2; acid phosphatase-like protein 2; FLJ23751
• Gene ID: 92370
• mRNA Refseq: NM_001037172
• Protein Refseq: NP_001032249
• UniProt ID: Q8TE99
• Chromosome Location: 3q23
• Pathway: NAD phosphorylation and dephosphorylation, organism-specific biosystem; NAD salvage pathway II, organism-specific biosystem
• Function: acid phosphatase activity; hydrolase activity

Reviews

04/05/2021

Just as they described on the site the package

03/20/2021

Good product.

12/10/2020

Workes great, very good product for a nice price.

07/31/2020

Fast delivery. Happy with purchase.

07/12/2018

This product is exactly as advertised with no issues.

Q&As

How does ACPL2 react with (+)-schisandrin B?

Schizandrin B inhibits the reaction [Carbon Tetrachloride results in decreased expression of PXYLP1 mRNA]

Could please list three molecular functions of ACPL2?

Lipid binding, oxidoreductase, and RNA binding.

How deos ACPL2 response to the phosphatase activity and acid phosphatase activity?

Responsible for the 2-O-dephosphorylation of xylose in the glycosaminoglycan-protein linkage region of proteoglycans thereby regulating the amount of mature glycosaminoglycan (GAG) chains.

What kind of diseases associated with ACPL2 gene?

 Diseases associated with ACPL2 include Ehlers-Danlos Syndrome, Musculocontractural Type, 2 and Spondyloepimetaphyseal Dysplasia With Joint Laxity, Type 1, With Or Without Fractures.

What phenotypes occur when affected by ACPL2?

Body height, body fat distribution, and abnormality of refraction, etc.

What biological process (gene ontology) does ACPL2 gene involved?

They are glycosaminoglycan biosynthetic process, positive regulation of heparan sulfate proteoglycan biosynthetic process, dephosphorylation, and chondroitin sulfate proteoglycan biosynthetic process.

What is the bio-physicochemical properties of ACPL2 gene?

pH dependence: Optimum pH is 5.8.

How does ACPL2 influence the 1,2-dimethylhydrazine?

1, 2-Dimethylhydrazine results in decreased expression of PXYLP1 mRNA

How about he mRNA expression of ACPL2 gene?

Tissue specificity: Widely expressed. Strongly expressed in spleen, fetal liver, moderately in placenta, pancreas, kidney, thymus and colon.

Could please some protein tissue co-expression partners for ACPL2 gene?

C4orf19, TNFAIP1, XKR8, KCNJ2, KDF1, BMP5.