Recombinant Human ACR Protein (Ile43-Gln343), N-His-tagged

• Cat.No.: ACR-0505H
• Product Overview: Recombinant human ACR (Ile43-Gln343) was fused with the N-His tag and was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Lyophilized powder/frozen liquid
• Molecular Mass: 35.43 kDa
• Protein Length: Ile43-Gln343
• Purity: >90% as determined by SDS-PAGE
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 centigrade for one week. Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: 0.01M PBS, pH 7.4, 0.02% NLS
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Gene Name: ACR acrosin [ Homo sapiens (human) ]
• Official Symbol: ACR
• Synonyms: ACR; acrosin; preproacrosin; proacrosin
• Gene ID: 49
• mRNA Refseq: NM_001097
• Protein Refseq: NP_001088
• MIM: 102480
• UniProt ID: P10323

Reviews

09/23/2022

The delivery speed is very fast, and I can start my experiment quickly.

04/01/2021

The protein quality is very high and can meet my experimental needs.

09/24/2020

The packaging is good, and the protein has not been damaged.

07/03/2018

The product description is very clear, and I can easily use the protein.

06/05/2018

The manufacturer provides excellent technical support and can solve my problems.

Q&As

What is the structure of the Acr protein?

The Acr protein is a transmembrane protein that spans the bacterial cell membrane and pumps antibiotics out of the cell.

How is the expression of the Acr gene regulated?

The expression of the Acr gene can be regulated by several mechanisms, including transcriptional regulation and post-transcriptional regulation.

How does the Acr gene confer antibiotic resistance?

The Acr protein pumps antibiotics out of bacterial cells, which reduces the concentration of the antibiotic inside the cell and makes it less effective.

What type of antibiotic resistance does the Acr gene confer?

The Acr gene confers resistance to multiple types of antibiotics, including tetracyclines and fluoroquinolones.

Are there any inhibitors of the Acr protein?

Yes, there are several inhibitors of the Acr protein that have been developed, including phenylalanine-arginine beta-naphthylamide (PAβN) and carbonyl cyanide m-chlorophenylhydrazone (CCCP).

How does horizontal gene transfer contribute to the spread of the Acr gene?

The Acr gene can be transferred between bacterial cells through horizontal gene transfer mechanisms, such as conjugation, transformation, and transduction.

What is the role of the periplasmic adapter protein in the efflux pump system?

The periplasmic adapter protein is a key component of the efflux pump system that links the outer membrane channel protein to the Acr protein in the inner membrane.

How is the expression of the Acr gene regulated at the post-transcriptional level?

The expression of the Acr gene can be regulated at the post-transcriptional level by small RNAs that bind to the mRNA transcript and either stabilize or degrade it.

What mutations in the Acr gene are associated with antibiotic resistance?

Mutations in the coding sequence of the Acr gene can affect the function of the Acr protein and contribute to antibiotic resistance, particularly mutations that affect the extracellular loop region.

Can the Acr gene be used as a diagnostic tool for antibiotic resistance?

Yes, the Acr gene can be used as a diagnostic tool for antibiotic resistance by detecting the presence of the gene in bacterial samples.