Recombinant Human ACSL1

• Cat.No.: ACSL1-26479TH
• Product Overview: Recombinant fragment: PKPLKPPCDL SMQSVEVAGS GGARRSALLD SDEPLVYFYD DVTTLYEGFQ RGIQVSNNGP CLGSRKPDQP YEWLSYKQVA ELSECIGSAL IQKGFKTA of Human ACSL1 (amino acids 48-145) with N terminal proprietary tag, 36.41 kDa.

Specification

• Description: The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation.
• Protein length: 98 amino acids
• Molecular Weight: 36.410kDa inclusive of tags
• Source: Wheat germ
• Tissue specificity: Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas.
• Form: Liquid
• Purity: Proprietary Purification
• Storage buffer: pH: 8.00Constituents:0.3% Glutathione, 0.79% Tris HCl
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: PKPLKPPCDLSMQSVEVAGSGGARR SALLDSDEPLVYFYDDVTTLYEGFQ RGIQVSNNGPCLGSRKPDQPYEWLS YKQVAELSECIGSALIQKGFKTA
• Sequence Similarities: Belongs to the ATP-dependent AMP-binding enzyme family.

Gene Information

• Gene Name: ACSL1 acyl-CoA synthetase long-chain family member 1 [ Homo sapiens ]
• Official Symbol: ACSL1
• Synonyms: ACSL1; acyl-CoA synthetase long-chain family member 1; FACL2, fatty acid Coenzyme A ligase, long chain 2; long-chain-fatty-acid--CoA ligase 1; ACS1; FACL1; LACS; LACS1; LACS2; lignoceroyl CoA synthase; long chain fatty acid coenzyme A ligase 1
• Gene ID: 2180
• mRNA Refseq: NM_001995
• Protein Refseq: NP_001986
• MIM: 152425
• Uniprot ID: P33121
• Chromosome Location: 4q35
• Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Fatty Acid Beta Oxidation, organism-specific biosystem; Fatty Acid Biosynthesis, organism-specific biosystem; Fatty Acyl-CoA Biosynthesis, organism-specific biosystem
• Function: ATP binding; ligase activity; long-chain fatty acid-CoA ligase activity; nucleotide binding

Reviews

10/11/2021

Outstanding quality and service. Would give 10 stars if I could.

10/16/2020

The recombinant protein worked perfectly for my experiment. Thank you!

07/23/2019

Excellent value for the price. Will be a repeat customer.

12/03/2018

Thank you for providing such a great product.

03/03/2018

I've ordered from many suppliers in the past, but this company stands out for its exceptional quality and service.

Q&As

What is the relationship between ACSL1 and the gut microbiome?

ACSL1 has been shown to be involved in the regulation of the gut microbiome by modulating the composition of the microbial community and the production of microbial metabolites. It is thought to do this by altering the availability of fatty acids and other nutrients in the gut environment.

What is the role of ACSL1 in mitochondrial function?

ACSL1 is involved in the regulation of mitochondrial function by modulating the production of reactive oxygen species (ROS) and the activity of the electron transport chain. It has been shown to be important for the maintenance of mitochondrial membrane potential and energy production in cells.

What inhibitors have been developed to target ACSL1?

Several inhibitors of ACSL1 have been developed, including triacsin C and thioesterase inhibitors such as N-arylthiourea and dithioester analogs. These inhibitors have shown promise in preclinical studies as potential therapeutics for metabolic diseases and cancer.

How does ACSL1 contribute to the regulation of lipid metabolism during fasting?

ACSL1 is upregulated in response to fasting and is thought to contribute to the regulation of lipid metabolism during this time by promoting the oxidation of fatty acids for energy production and the synthesis of ketone bodies.

What diseases or disorders are associated with mutations in the ACSL1 gene?

Mutations in the ACSL1 gene have been associated with a variety of diseases and disorders, including obesity, diabetes, and non-alcoholic fatty liver disease.

How is the activity of ACSL1 regulated by phosphorylation?

ACSL1 can be phosphorylated by various kinases, such as PKC and AMPK, which can alter its activity and localization. Phosphorylation of ACSL1 has been shown to decrease its activity and promote its translocation from the cytoplasm to the nucleus.

What is the mechanism of action of triacsin C, an inhibitor of ACSL1?

Triacsin C inhibits the activity of ACSL1 by binding to its acyl-CoA binding site and preventing the formation of the acyl-adenylate intermediate. This prevents the conversion of fatty acids to their acyl-CoA derivatives, leading to a decrease in lipid synthesis and storage.

What is the role of ACSL1 in the synthesis of eicosanoids?

ACSL1 plays a role in the synthesis of eicosanoids, which are important signaling molecules involved in a variety of physiological processes, such as inflammation and blood clotting. ACSL1 is involved in the activation of arachidonic acid, a precursor of eicosanoids, by converting it to arachidonyl-CoA.

How is ACSL1 involved in the regulation of inflammation?

ACSL1 has been shown to be involved in the regulation of inflammation by modulating the production of pro-inflammatory mediators, such as cytokines and chemokines. It is thought to do this by altering the lipid composition of cellular membranes and influencing the signaling pathways involved in inflammation.

How is ACSL1 involved in the regulation of lipid droplet formation?

ACSL1 has been shown to be involved in the regulation of lipid droplet formation by promoting the esterification of fatty acids to neutral lipids, which can then be stored in lipid droplets. It has also been shown to interact with other proteins.