Recombinant Human ADAD1 293 Cell Lysate

• Cat.No.: ADAD1-9040HCL

Specification

• Description: Antigen standard for adenosine deaminase domain containing 1 (testis-specific) (ADAD1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ADAD1 adenosine deaminase domain containing 1 (testis-specific) [ Homo sapiens ]
• Official Symbol: ADAD1
• Synonyms: ADAD1; adenosine deaminase domain containing 1 (testis-specific); adenosine deaminase domain-containing protein 1; Tenr; testis nuclear RNA-binding protein; FLJ32741
• Gene ID: 132612
• mRNA Refseq: NM_001159285
• Protein Refseq: NP_001152757
• MIM: 614130
• UniProt ID: Q96M93
• Chromosome Location: 4q27
• Function: adenosine deaminase activity; double-stranded RNA binding

Reviews

10/18/2022

The after-sales technical expert was able to solve my problem in a timely manner.

01/07/2022

I customized the ADAD1 with other tags and it took a little longer to arrive, but it was within my acceptance range.

08/04/2021

The protein concentration is in accordance with the product information and the delivery is fast.

Q&As

Is there any discount for buying ADAD1 and ADAD2 together?

We recently had a sale on all our products, and you can get both of these products at a reduced price during the event period.

Can I customize ADAD1 with N-terminal tag?

Yes, you can directly communicate with us for specific customization requirements.

Do you provide ADAD1-related antibody products?

We do not provide related antibody products, but our experts can give you some advice on antibody selection based on product characteristics.

Does the ADAD1 protein have enzymatic activity?

The ADAD1 protein has no enzymatic activity.

Can ADAD1 of specific band be customized?

Yes, you can directly communicate with our experts for specific customization requirements.

Relationship between ADAD1 and ADAD2?

Both ADAD1 and ADAD2 belong to the testis-specific adenosine deaminase domain-containing (ADAD) protein family.

Can ADAD1 protein add his tag?

We can customize the tag according to your needs.

Is there any relevant content about product handling in the product manual of ADAD1?

The product manual will contain product handling related content, and you can also directly consult our technical experts for any questions about product handling.

In addition to fertility-related research, ADAD1 is currently used in the research of which diseases?

ADAD1 is currently also used in the research of colon cancer, ovarian cancer, etc.

What is the difference between the expression locations of ADAD1 and ADAD2?

ADAD1 is mainly expressed in the post-meiotic round spermatid, and ADAD2 is mainly expressed in mid-to-late pachytene spermatocytes.

How soon after my purchase will it be shipped?

If there is no customization requirement, we will ship it as soon as possible after your successful purchase, but if there is a customization requirement, the delivery time will be later.