Recombinant Human ADAL 293 Cell Lysate

• Cat.No.: ADAL-9039HCL

Specification

• Description: Antigen standard for adenosine deaminase-like (ADAL), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ADAL adenosine deaminase-like [ Homo sapiens ]
• Official Symbol: ADAL
• Synonyms: ADAL; adenosine deaminase-like; adenosine deaminase-like protein; FLJ44620; DKFZp313B2137
• Gene ID: 161823
• mRNA Refseq: NM_001012969
• Protein Refseq: NP_001012987
• UniProt ID: Q6DHV7
• Chromosome Location: 15q15.3
• Function: adenosine deaminase activity; hydrolase activity; metal ion binding

Reviews

02/27/2023

I bought adal during the promotion period, the price is cheaper, the protein purity is high, and the experimental results are good.

08/17/2021

The after-sales service is particularly good, and the technical expert helped me solve several problems in the experiment.

04/24/2021

I customized ADAL protein for use in my research and the product parameters met the requirements and the price was reasonable.

04/22/2021

Very good protein product, and the accompanying product manual is quite detailed.

07/06/2020

High quality protein, good after-sales service, and professional technicians.

Q&As

Does ADAL-1274M participate in this promotion?

ADAL-1274M participates in this promotion.

I purchased ADAL-271H for western blotting, can you provide some suggestions for antibody selection?

Our technical experts will provide you with relevant advice.

Will the His tag affect the activity of the protein?

In general, tags with small molecular weights have very little effect on protein activity.

I plan to buy ADAL protein for western blotting, how should I choose ADAL protein with different cat no.?

This needs to be selected according to your research needs, and our technical experts can provide you with assistance in this regard.

Can zebrafish ADAL only add tags other than His?

We can also add other tags according to your requirements.

Will the His tag affect the downstream application of the protein?

The molecular weight of the His tag is small and will not affect the downstream application of the protein.

Is ADAL protein effective for WB? Is the band clear?

Our customer obtained clear bands in western blotting with ADAL.

What function does ADAL-1 have?

ADAL-1 is responsible for the removal of a wide range of moieties from the base rings of purine nucleotide and nucleotide analogs.

How long will it take after I purchase ADAL?

We will ship the products in stock as soon as possible after purchase, and the specific arrival time is related to your geographical location.

Can mouse ADAL remove endotoxin?

Yes, we can custom produce mouse ADAL according to your requirements.