Recombinant Human ADCY8 293 Cell Lysate

• Cat.No.: ADCY8-9020HCL

Specification

• Description: Antigen standard for adenylate cyclase 8 (brain) (ADCY8) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ADCY8 adenylate cyclase 8 (brain) [ Homo sapiens ]
• Official Symbol: ADCY8
• Synonyms: ADCY8; adenylate cyclase 8 (brain); ADCY3; adenylate cyclase type 8; AC8; HBAC1; adenylyl cyclase 8; ATP pyrophosphate-lyase 8; adenylyl cyclase-8, brain; adenylate cyclase type VIII; ca(2+)/calmodulin-activated adenylyl cyclase
• Gene ID: 114
• mRNA Refseq: NM_001115
• Protein Refseq: NP_001106
• MIM: 103070
• UniProt ID: P40145
• Chromosome Location: 8q24
• Pathway: Activation of GABAB receptors, organism-specific biosystem; Activation of NMDA receptor upon glutamate binding and postsynaptic events, organism-specific biosystem; Adenylate cyclase activating pathway, organism-specific biosystem; Adenylate cyclase inhibitory pathway, organism-specific biosystem; Aquaporin-mediated transport, organism-specific biosystem; Bile secretion, organism-specific biosystem; Bile secretion, conserved biosystem
• Function: ATP binding; GTPase activity; adenylate cyclase activity; calcium- and calmodulin-responsive adenylate cyclase activity; metal ion binding; nucleotide binding

Q&As

Are there any other diseases that can be treated by targeting ADCY8 protein?

Yes, targeting ADCY8 protein may have therapeutic potential in other diseases. For example, modulating ADCY8 activity may be beneficial in the treatment of neurological disorders such as Parkinson's disease and schizophrenia, as well as in obesity and certain cancers.

Are there any current drugs that target ADCY8 protein?

There are currently no drugs available that specifically target ADCY8 protein. However, drugs that affect the cAMP-PKA signaling pathway, such as glucagon-like peptide-1 (GLP-1) agonists used in the treatment of type 2 diabetes, indirectly affect ADCY8 activity.

Can targeting ADCY8 protein be used as a therapeutic strategy for diabetes?

Yes, targeting ADCY8 protein may be a potential therapeutic strategy for diabetes. Modulating ADCY8 activity can regulate insulin secretion and improve glucose homeostasis. However, further research is needed to determine the safety and efficacy of modulating ADCY8 in humans.

What are the challenges of developing drugs that target ADCY8 protein?

Developing drugs that specifically target ADCY8 protein can be challenging due to its complex regulation and interaction with other signaling pathways. Additionally, targeting ADCY8 may affect other physiological processes that could lead to unwanted side effects. Further research is needed to fully understand ADCY8's role in various diseases and to develop safe and effective therapeutics.

What are the potential side effects of targeting ADCY8 protein?

The potential side effects of targeting ADCY8 protein are not well known as this is an emerging area of research. However, as with any drug targeting a specific protein, potential side effects could include off-target effects, changes in related signaling pathways, and potential toxicity.

How can ADCY8 protein be used in obesity treatment?

ADCY8 is being explored as a potential target in obesity treatment due to its role in regulating energy metabolism and appetite control. Modulating ADCY8 activity may help to improve energy expenditure and reduce food intake, leading to weight loss.

Can measuring ADCY8 protein levels be used for diagnosis of diseases?

Measuring ADCY8 protein levels alone may not be sufficient for diagnosis of diseases. However, measuring ADCY8 activity or levels in conjunction with other diagnostic tests may provide additional information for disease diagnosis, prognosis, and treatment.

Can ADCY8 protein be used to treat neurological disorders?

ADCY8 is being studied as a potential therapeutic target in neurological disorders, such as Parkinson's disease and schizophrenia, due to its role in neurotransmitter signaling and neuronal function. Modulating ADCY8 activity may help to improve synaptic plasticity and reduce cognitive impairment in these disorders.

Are there any drugs that target ADCY8 protein?

There are currently no drugs that specifically target ADCY8 protein. However, compounds that modulate ADCY8 activity are being developed and tested for their therapeutic potential.

How is ADCY8 protein regulated in the body?

ADCY8 protein is regulated by a variety of factors, including hormonal and neural signals. For example, glucagon-like peptide-1 (GLP-1) and glucose signal through G protein-coupled receptors to activate ADCY8 and stimulate insulin secretion. Other factors, such as dopamine and calcium ions, can also modulate ADCY8 activity.

Is it possible to supplement ADCY8 protein for therapeutic purposes?

It is currently not possible to supplement ADCY8 protein for therapeutic purposes. However, the development of ADCY8 agonists or inhibitors may help to modulate ADCY8 activity for therapeutic benefit.

Can ADCY8 protein be used as a diagnostic tool?

ADCY8 protein is not typically used as a direct diagnostic tool. However, measuring ADCY8 expression levels or genetic variants associated with ADCY8 may be useful in predicting disease outcomes and identifying patients who may benefit from certain treatments.

Are there any genetic mutations associated with ADCY8?

Yes, several genetic variants in the ADCY8 gene have been associated with various diseases and traits, including obesity, diabetes, and schizophrenia. These variants can affect ADCY8 expression levels, activity, and function, leading to altered signaling pathways.

How is ADCY8 protein being studied in research?

ADCY8 protein is being studied in various research settings, including preclinical studies in animal models and in vitro studies to understand its role in disease and potential therapeutic targets. Clinical trials may also be conducted in the future to test the efficacy and safety of drugs targeting ADCY8 in humans.