Recombinant Human ADSSL1 293 Cell Lysate
Cat.No. : | ADSSL1-8993HCL |
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Description : | Antigen standard for adenylosuccinate synthase like 1 (ADSSL1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | ADSSL1 adenylosuccinate synthase like 1 [ Homo sapiens ] |
Official Symbol : | ADSSL1 |
Synonyms : | ADSSL1; adenylosuccinate synthase like 1; adenylosuccinate synthetase isozyme 1; FLJ38602; adSS 1; AMPSase 1; IMP--aspartate ligase 1; M-type adenylosuccinate synthetase; adenylosuccinate synthetase, basic isozyme; adenylosuccinate synthetase, muscle isozyme; |
Gene ID : | 122622 |
mRNA Refseq : | NM_152328 |
Protein Refseq : | NP_689541 |
MIM : | 612498 |
UniProt ID : | Q8N142 |
Chromosome Location : | 14q32.33 |
Pathway : | Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of nucleotides, organism-specific biosystem; Purine metabolism, organism-specific biosystem; Purine metabolism, organism-specific biosystem; |
Function : | GTP binding; adenylosuccinate synthase activity; ligase activity; magnesium ion binding; nucleotide binding; phosphate ion binding; |
Products Types
◆ Recombinant Protein | ||
ADSSL1-370M | Recombinant Mouse ADSSL1 Protein, His (Fc)-Avi-tagged | +Inquiry |
Adssl1-550M | Recombinant Mouse Adssl1 Protein, MYC/DDK-tagged | +Inquiry |
ADSSL1-1392M | Recombinant Mouse ADSSL1 Protein | +Inquiry |
Adssl1-3189M | Recombinant Mouse Adssl1, His-tagged | +Inquiry |
ADSSL1-392H | Recombinant Human ADSSL1 Protein, GST-tagged | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionThe subcellular localization of ADSSL1 protein is primarily within mitochondria, facilitated by mitochondrial targeting sequences and import machinery.
Post-translational modifications, such as phosphorylation or acetylation, and regulatory factors like metabolic signals or protein-protein interactions, may modulate the activity or stability of ADSSL1 protein.
ADSSL1 protein can interact with other enzymes involved in purine metabolism, forming complexes that regulate nucleotide synthesis and energy metabolism.
Genetic variations or mutations in the ADSSL1 gene can impact the expression or function of ADSSL1 protein, potentially leading to disorders associated with purine metabolism abnormalities.
ADSSL1 protein plays a critical role in purine nucleotide metabolism, contributing to the deamination and recycling of adenylosuccinate to maintain nucleotide homeostasis.
ADSSL1 protein contains conserved structural domains, including a catalytic domain and nucleotide-binding motifs, that contribute to its enzymatic activity and substrate specificity.
The enzymatic activity of ADSSL1 protein, catalyzing the conversion of adenylosuccinate to AMP, can be measured using enzymatic assays that quantify substrate utilization and product formation.
Experimental approaches such as knockout or overexpression studies have been utilized to investigate the functional significance of ADSSL1 protein, revealing its role in cellular metabolism and energy balance.
ADSSL1 protein exhibits tissue-specific expression, with higher levels observed in skeletal muscle, heart, and brain. Factors involved in its regulation include transcriptional control and signaling pathways.
Dysregulation or dysfunction of ADSSL1 protein can disrupt cellular purine metabolism, leading to imbalances in nucleotide levels and potential contributions to diseases such as neurodegenerative disorders or metabolic syndromes.
Customer Reviews (3)
Write a reviewExploring protein-protein interactions involved in intracellular trafficking.
Detecting protein-protein interactions in membrane receptor signaling.
Understanding protein-protein interaction networks in DNA repair processes.
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