Recombinant Human AEN 293 Cell Lysate
Cat.No. : | AEN-8992HCL |
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Description : | Antigen standard for apoptosis enhancing nuclease (AEN) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | AEN apoptosis enhancing nuclease [ Homo sapiens ] |
Official Symbol : | AEN |
Synonyms : | AEN; apoptosis enhancing nuclease; interferon stimulated exonuclease gene 20kDa like 1 , ISG20L1; apoptosis-enhancing nuclease; FLJ12484; FLJ12562; interferon-stimulated 20 kDa exonuclease-like 1; interferon stimulated exonuclease gene 20kDa-like 1; ISG20L1; pp12744; |
Gene ID : | 64782 |
mRNA Refseq : | NM_022767 |
Protein Refseq : | NP_073604 |
MIM : | 610177 |
UniProt ID : | Q8WTP8 |
Chromosome Location : | 15q26.1 |
Function : | exonuclease activity; hydrolase activity; nucleic acid binding; |
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AEN-207R | Recombinant Rat AEN Protein, His (Fc)-Avi-tagged | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionThe subcellular localization of AEN protein is determined by specific mechanisms and targeting sequences, which facilitate its transport and localization to specific cellular compartments.
Dysregulation or dysfunction of AEN protein can disrupt cellular functions, such as impaired DNA repair or altered apoptotic responses, and contribute to the development of diseases like cancer or neurodegenerative disorders.
Genetic variations or mutations in the AEN gene may impact the expression or function of AEN protein, potentially influencing cellular processes or disease susceptibility.
AEN protein may interact with other proteins, such as DNA repair factors or apoptotic regulators, forming protein complexes that influence cellular processes such as DNA repair or cell death.
AEN protein is implicated in specific cellular processes such as DNA repair and apoptosis, where it may play a role in maintaining genomic stability or regulating cell survival.
Experimental techniques such as molecular biology, cell biology, and biochemical assays can be employed to investigate the functional significance of AEN protein, including DNA repair assays or apoptosis assays.
Post-translational modifications or regulatory factors that modulate the activity or stability of AEN protein are not currently identified. Further research is necessary to explore these regulatory mechanisms.
The specific structural features or domains critical for the function or interaction of AEN protein are currently unknown, and further studies are needed to determine their role in cellular processes and molecular interactions.
The enzymatic activity or molecular function of AEN protein is currently unknown, and further experimental assays are needed to investigate its activity and molecular roles.
The tissue-specific expression patterns of AEN protein vary across different organs and cell types. Factors regulating its expression levels include transcriptional control and signaling pathways.
Customer Reviews (3)
Write a reviewExploring protein-protein interactions involved in neurotransmitter signaling.
Characterizing protein-protein interactions involved in chromosomal organization.
Determining protein-protein interaction interfaces using F枚rster resonance energy transfer.
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