Recombinant Human AES, His-tagged

• Cat.No.: AES-3479H
• Product Overview: Recombinant human AES protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Cat. No.: AES-3479H
• Description: Amino-terminal enhancer of split, also known AES, belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. This protein is expressed predominately in fetal brain, liver, lung, heart and kidney and in adult muscle. In addition, AES can repress NFκB-regulated gene expression and is thought to play an important role in initiating and maintaining cell differentiation events.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol
• Molecular Weight: 24.1 kDa (217aa) confirmed by MALDI-TOF
• Purity: > 95% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MMFPQSRHSG SSHLPQQLKF TTSDSCDRIK DEFQLLQAQY HSLKLECDKL ASEKSEMQRH YVMYYEMSYG LNIEMHKQAE IVKRLNGICA QVLPYLSQEH QQQVLGAIER AKQVTAPELN SIIRQQLQAH QLSQLQALAL PLTPLPVGLQ PPSLPAVSAG TGLLSLSALG SQAHLSKEDK NGHDGDTHQE DDGEKSD
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: AES amino-terminal enhancer of split [ Homo sapiens ]
• Synonyms: amino-terminal enhancer of split; GRG; ESP1; GRG5; TLE5; AES-1; AES-2; AES; amino enhancer of split; gp130-associated protein GAM
• Gene ID: 166
• mRNA Refseq: NM_001130
• Protein Refseq: NP_001121
• MIM: 600188
• UniProt ID: Q08117
• Chromosome Location: 19p13.3
• Function: protein binding; transcription corepressor activity; transcription corepressor activity

Reviews

09/12/2022

Understanding protein-protein interaction networks in oxidative stress responses.

06/15/2022

Detecting protein-protein interactions in ion transport and homeostasis.

07/27/2019

Exploring protein-protein interactions involved in cytoskeleton organization.

Q&As

Are there any known post-translational modifications or regulatory factors that modulate the activity or stability of AES protein?

Post-translational modifications, such as phosphorylation or acetylation, and regulatory factors can modulate the activity or stability of AES protein, influencing its function in gene regulation and cellular processes.

What experimental techniques or assays have been employed to study the functional significance of AES protein, such as gene expression analysis or chromatin immunoprecipitation?

Experimental techniques such as gene expression analysis, chromatin immunoprecipitation, or functional genomics approaches have been employed to investigate the functional significance of AES protein in gene regulation and cellular processes.

Can AES protein be targeted or manipulated pharmacologically to modulate cellular processes or potentially serve as a therapeutic target for diseases associated with dysregulated gene expression or abnormal cell growth?

AES protein could be a potential pharmacological target to modulate gene expression, cellular processes, or serve as a therapeutic target for diseases associated with dysregulated gene expression or abnormal cell growth. Further research is needed to explore its therapeutic potential and develop targeted interventions.

How does dysregulation or dysfunction of AES protein affect cellular functions, developmental processes, or disease development?

Dysregulation or dysfunction of AES protein can disrupt cellular functions, developmental processes, and contribute to disease development, such as cancer, neurodegenerative disorders, or developmental disorders.

What are the cellular and subcellular localization patterns of AES protein, and how are they determined experimentally?

The cellular and subcellular localization patterns of AES protein can vary across different cell types and developmental stages. Experimental techniques such as immunofluorescence microscopy or subcellular fractionation can be used to determine its localization.

What are the transcriptional regulatory mechanisms that control the expression of AES protein in different cell types or under specific physiological conditions?

The expression of AES protein is regulated at the transcriptional level by specific promoters and transcription factors. Cellular signaling pathways and environmental cues can influence its expression in a context-dependent manner.

Are there any genetic variations or mutations in the AES gene that impact the expression or function of AES protein, and what are the implications for cellular processes?

Genetic variations or mutations in the AES gene may impact the expression or function of AES protein, potentially leading to dysregulated gene expression, altered cellular processes, or disease susceptibility.

Does AES protein interact with specific molecules or participate in protein complexes, and what are the functional consequences of these interactions?

AES protein may interact with specific molecules, such as transcription factors or chromatin modifiers, forming protein complexes that modulate gene expression and cellular processes.

How does AES protein modulate gene expression and transcriptional activity, and what are the underlying molecular mechanisms?

AES protein modulates gene expression and transcriptional activity by interacting with transcriptional regulators, co-repressors, or chromatin remodeling complexes, potentially leading to transcriptional repression or modulation of target genes.

What is the role of AES protein in cellular processes such as cell proliferation, differentiation, or apoptosis, and how is this function assessed experimentally?

AES protein plays a role in cellular processes such as cell proliferation, differentiation, or apoptosis, potentially through its interactions with transcription factors or modulation of gene expression. Experimental approaches including loss-of-function studies or functional assays can assess its function.