Recombinant Human AGAP5, His-tagged

• Cat.No.: AGAP5-3616H
• Product Overview: Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 5 (AGAP5)

Specification

• Source: E. Coli or Yeast
• Species: Human
• Tag: His
• Form: This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
• Protein length: 686
• Purity: >90%
• Notes: Small volumes of AGAP5 recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
• Storage: Store at -20 degree C. For extended storage, store at -20 or -80 degree C.
• Storage Buffer: PBS pH 7.4, 50% glycerol
• Warning: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Gene Information

• Gene Name: AGAP5 ArfGAP with GTPase domain, ankyrin repeat and PH domain 5 [ Homo sapiens ]
• Official Symbol: AGAP5
• Synonyms: CTGLF2; arf-GAP with GTPase, ANK repeat and PH domain-containing protein 5; AGAP-5; centaurin-gamma-like family member 2; centaurin, gamma-like family, member 2
• Gene ID: 729092
• mRNA Refseq: NM_001144000
• Protein Refseq: NP_001137472
• UniProt ID: A6NIR3
• Chromosome Location: 10q22.2
• Function: ARF GTPase activator activity; zinc ion binding

Reviews

02/06/2022

 Consistently achieving specific outcomes validated the product’s essential role in our studies.

11/22/2021

Agap5 consistently downregulated the expression of inflammatory markers.

04/23/2021

Our experiments with Agap5 showed no signs of protein aggregation.

10/31/2017

 The product’s unique traits propelled our research to new levels of innovation.

12/10/2016

Using Protein Agap5, we obtained uniform results in various experimental conditions.

Q&As

What other functions besides SMA-related modifications might AGAP5 have?

Apart from potential roles in SMA modification, AGAP5's involvement in membrane trafficking and cellular organization suggests broader functions in intracellular transport.

How is AGAP5 activity controlled?

AGAP5 activity is regulated by factors like post-translational modifications, protein-protein interactions, and potential cellular localization changes.

What is the role of AGAP5 in the context of SMA?

While the exact role of AGAP5 in SMA is not mentioned, its mutation suggests it could be part of the genetic landscape that influences the disease's severity.

Are there any known interactions between AGAP5 and other proteins?

AGAP5's ankyrin repeat domains indicate potential interactions with other proteins, possibly influencing its activity and cellular functions.

Are there any other functions of AGAP5?

Yes, AGAP5's ankyrin repeat and PH domain also suggest possible roles in protein-protein interactions and lipid binding, respectively.

Where is AGAP5 found in cells?

AGAP5 is primarily located at the trans-Golgi network and endosomes within cells.

What is the significance of the mutations in AGAP5?

The mutations in AGAP5 are noteworthy as they might contribute to altering the severity of SMA in the affected individuals, highlighting AGAP5's potential role as a genetic modifier.

Has AGAP5 been implicated in other diseases or conditions?

AGAP5's role in cellular processes makes it a candidate for involvement in other diseases, although specific connections beyond SMA might require further research.

How were the mutations in AGAP5 identified?**

The mutations in AGAP5 were likely identified through genetic sequencing and analysis of the patients' genomes, alongside mutations in TLL2 and VPS13A.

Can AGAP5 be a therapeutic target?

Given its involvement in cellular processes, AGAP5 could potentially be targeted for therapeutic interventions aimed at modulating membrane trafficking and related disorders.

Are there any diseases associated with AGAP5 dysfunction?

Although research is ongoing, AGAP5's dysregulation could potentially impact cellular trafficking processes and contribute to diseases like cancer and neurological disorders.

Are there any animal models used to study AGAP5 function?

Animal models, such as genetically modified mice or cell-based models, could be used to study AGAP5's function and its effects on cellular processes.

Could AGAP5 have different effects in different tissues or cell types?

Yes, the expression and function of AGAP5 might vary across tissues and cell types, contributing to different outcomes in different contexts.

What is the structure of AGAP5?

AGAP5 comprises an ARF-GAP domain responsible for GTPase regulation, ankyrin repeat domains for protein interactions, and a PH domain possibly involved in lipid binding.

How might AGAP5 interact with other genetic modifiers in SMA?

It's possible that AGAP5 interacts with other genetic modifiers to collectively impact the severity of SMA, demonstrating the complex genetic nature of the disease.

How does AGAP5 regulate membrane trafficking?

AGAP5 influences vesicle budding, cargo sorting, and membrane fusion by modulating the activity of ARF6, a GTPase involved in endocytic pathways.

What is AGAP5 protein?

AGAP5, also known as Arf-GAP with GTPase domain, Ankyrin repeat, and PH domain 5, is a protein involved in cellular processes like membrane trafficking and cytoskeleton regulation.

How does AGAP5 relate to the SMA discordant family case?

In the SMA discordant family case, mutations were identified in the AGAP5 gene among other genes. These mutations could play a role in modifying the severity of SMA.

What does AGAP5 do?

AGAP5 functions as a GTPase-activating protein (GAP) that regulates the activity of small GTPases, including ADP-ribosylation factor 6 (ARF6). It controls vesicle trafficking and membrane dynamics.

Why is AGAP5 important?

AGAP5's role in membrane trafficking and cellular organization makes it crucial for maintaining proper intracellular transport and compartmentalization.