Recombinant Human AGFG1 293 Cell Lysate

• Cat.No.: AGFG1-8982HCL

Specification

• Description: Antigen standard for ArfGAP with FG repeats 1 (AGFG1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: AGFG1 ArfGAP with FG repeats 1 [ Homo sapiens ]
• Official Symbol: AGFG1
• Synonyms: AGFG1; ArfGAP with FG repeats 1; HIV 1 Rev binding protein , HRB; arf-GAP domain and FG repeat-containing protein 1; RAB; RIP; rev-interacting protein; HIV-1 Rev binding protein; HIV-1 Rev-binding protein; nucleoporin-like protein RIP; hRIP, Rev interacting protein; rev/Rex activation domain-binding protein; Rab, Rev/Rex activation domain-binding protein; arf-GAP domain and FG repeats-containing protein 1; HRB; MGC116938; MGC116940; DKFZp686I15205
• Gene ID: 3267
• mRNA Refseq: NM_001135187
• Protein Refseq: NP_001128659
• MIM: 600862
• UniProt ID: P52594
• Chromosome Location: 2q36
• Pathway: Influenza A, organism-specific biosystem; Influenza A, conserved biosystem
• Function: ARF GTPase activator activity; DNA binding; RNA binding; metal ion binding; protein binding; zinc ion binding

Reviews

08/03/2022

It’s stability across experiments ensured reliable functional assays.

01/04/2022

AGFG1’s consistent binding kinetics enhanced our understanding of interactions.

03/10/2017

 The value derived from the product’s usage justified its expenditure for scientific advancement.

01/07/2017

 The product’s cost-effectiveness and clear guidelines were advantageous for our experiments.

05/10/2016

The protein product’s consistent outcomes supported the reproducibility of our studies.

Q&As

How does AGFG1 affect Golgi apparatus?

AGFG1 plays a role in Golgi structure and vesicle trafficking between Golgi compartments.

Can AGFG1 influence membrane dynamics?

AGFG1's functions include regulating membrane dynamics and trafficking pathways.

Does AGFG1 regulate protein sorting?

Yes, AGFG1 contributes to protein sorting and proper distribution within cells.

Can AGFG1 influence cellular response to stress?

AGFG1's functions could impact how cells respond to various stressors.

Can AGFG1 regulate clathrin-coated vesicles?

Yes, AGFG1 is involved in the formation and trafficking of clathrin-coated vesicles.

Are there connections between AGFG1 and cancer?

Research explores AGFG1's potential role in cancer progression through its functions.

How does AGFG1 relate to cellular secretion?

AGFG1's involvement in vesicle transport contributes to cellular secretion processes.

Can AGFG1 be targeted for therapeutic intervention?

Investigating AGFG1's role could reveal new targets for drug development in trafficking-related diseases.

How does AGFG1 affect cargo sorting at the trans-Golgi network?

AGFG1 plays a role in sorting cargo at the trans-Golgi network for subsequent transport.

What are the implications of AGFG1's interactions?

AGFG1 interacts with proteins involved in membrane trafficking and vesicle transport.

What's the relevance of AGFG1 in membrane recycling?

AGFG1 contributes to membrane recycling processes, ensuring proper membrane turnover.

What's the role of AGFG1 in cells?

AGFG1 participates in vesicle transport, Golgi organization, and membrane trafficking.

How is AGFG1 studied in research?

Research investigates AGFG1's functions and interactions, shedding light on cellular processes.

How does AGFG1 impact endosomal transport?

AGFG1 influences endosomal trafficking and the movement of cargo within cells.

How does AGFG1 contribute to cell homeostasis?

AGFG1's roles in membrane trafficking contribute to maintaining cellular balance.

What is the AGFG1 protein?

AGFG1 stands for "Arf-GAP with FG repeats 1," involved in intracellular trafficking.

How does AGFG1 affect vesicle budding?

AGFG1 influences vesicle budding and cargo packaging during intracellular transport.

Are there therapeutic applications for AGFG1?

AGFG1's role in cellular trafficking could have implications for drug delivery strategies.

Is AGFG1 linked to autophagy processes?

AGFG1's roles in vesicle transport could potentially intersect with autophagy pathways.

Is AGFG1 implicated in disease?

AGFG1's dysfunction could potentially contribute to cellular trafficking-related disorders.