Recombinant Human AGMAT 293 Cell Lysate

• Cat.No.: AGMAT-8978HCL

Specification

• Description: Antigen standard for agmatine ureohydrolase (agmatinase) (AGMAT) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: AGMAT agmatine ureohydrolase (agmatinase) [ Homo sapiens ]
• Official Symbol: AGMAT
• Synonyms: AGMAT; agmatine ureohydrolase (agmatinase); agmatinase, mitochondrial; FLJ23384; AUH
• Gene ID: 79814
• mRNA Refseq: NM_024758
• Protein Refseq: NP_079034
• UniProt ID: Q9BSE5
• Chromosome Location: 1p36.13
• Pathway: Agmatine biosynthesis, organism-specific biosystem; Arginine and proline metabolism, organism-specific biosystem; Arginine and proline metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Metabolism of polyamines, organism-specific biosystem
• Function: agmatinase activity; hydrolase activity; metal ion binding

Reviews

08/27/2022

 The protein product’s ability to induce apoptosis provided valuable insights into cell mechanisms.

04/17/2022

 Precise results from immunoprecipitation assays were attributed to the protein product’s purity.

02/26/2022

 Well-defined gel electrophoresis bands were indicative of the protein product’s pure form.

10/08/2018

 Protein interactions were effectively modulated by the protein product, broadening our understanding.

06/25/2017

The insights gained from the protein product justified its cost, offering value for money.

Q&As

How does AGMAT's deficiency impact polyamine levels?

AGMAT deficiency can lead to elevated agmatine levels and disrupted polyamine balance.

Can AGMAT's activity influence wound healing?

Yes, AGMAT's involvement in polyamine metabolism could impact wound healing processes.

What's the connection between AGMAT and agmatine?

AGMAT converts agmatine to putrescine, a crucial step in agmatine metabolism.

How does AGMAT contribute to cellular function?

AGMAT's activity helps regulate polyamine levels, impacting various cellular processes.

Can AGMAT impact brain health?

Yes, AGMAT's role in polyamine metabolism could have implications for brain function.

How is AGMAT deficiency inherited?

AGMAT deficiency is inherited in an autosomal recessive manner.

How is AGMAT's function conserved across species?

Research explores whether AGMAT's role is conserved in different organisms.

Can AGMAT's function be influenced by cellular signaling pathways?

Yes, cellular signaling pathways might regulate AGMAT's activity.

Can AGMAT's activity be modulated for therapeutic purposes?

Research investigates modulating AGMAT activity for potential therapeutic applications.

How does AGMAT impact cellular growth?

AGMAT's role in polyamine metabolism can influence cellular growth and proliferation.

What is the AGMAT protein?

AGMAT stands for "Agmatinase," an enzyme involved in the polyamine metabolism pathway.

How is AGMAT's enzymatic activity studied?

Research investigates AGMAT's enzymatic function and its implications.

Can AGMAT mutations lead to diseases?

Mutations in the AGMAT gene can cause AGMAT deficiency, impacting polyamine metabolism.

What's the role of AGMAT in polyamine metabolism?

AGMAT catalyzes the conversion of agmatine to putrescine, a step in polyamine breakdown.

Can AGMAT be targeted for therapeutic interventions?

Research explores AGMAT as a potential target in diseases related to polyamine metabolism.

Can AGMAT's deficiency affect cellular stress responses?

AGMAT deficiency might impact cellular responses to stress due to altered polyamine metabolism.

Where is AGMAT located in the cell?

AGMAT is located in the cytoplasm of cells.

How does AGMAT's activity relate to putrescine levels?

AGMAT's enzymatic activity directly affects putrescine levels in cells.

Is AGMAT involved in cancer research?

AGMAT's involvement in polyamine metabolism could be relevant in cancer studies.

How is AGMAT's deficiency diagnosed?

Diagnosis involves measuring AGMAT activity in blood or genetic testing.