Recombinant Human AGRN, His-tagged

• Cat.No.: AGRN-26658TH
• Product Overview: Recombinant fragment, corresponding to amino acids 1601-2045 of Human Agrin with a N terminal His tag; 52kDa.

Specification

• Description: This gene encodes one of several proteins that are critical in the development of the neuromuscular junction (NMJ), as identified in mouse knock-out studies. The encoded protein contains several laminin G, Kazal type serine protease inhibitor, and epidermal growth factor domains. Additional post-translational modifications occur to add glycosaminoglycans and disulfide bonds. In one family with congenital myasthenic syndrome affecting limb-girdle muscles, a mutation in this gene was found.
• Conjugation: HIS
• Source: E. coli
• Form: Lyophilised:Reconstitution with 127 μl aqua dest.
• Storage buffer: Preservative: NoneConstituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: APCRVLPEGGAQCECPLGREGTFCQ TASGQDGSGPFLADF NGFSHLELRGLHTFARDLGEKMALE VVFLARGPSGLLL YNGQKTDGKGDFVSLALRDRRLEFR YDLGKGAAVIRSR EPVTLGAWTRVSLERNGRKGALRVG DGPRVLGESPVPHTV LNLKEPLYVGGAPDFSKLARAAAVS SGFDGAIQLVSLG GRQLLTPEHVLRQVDVTSFAGHPCT RASGHPCLNGASC VPREAAYVCLCPGGFSGPHCEKGLV EKSAGDVDTLAFDGRTFVEYLNAVT ESEKALQSNHFELSLRTEATQGLVL WSG KATERADYVALAIVDGHLQLSYNLG SQPVVLRSTVPVN TNRWLRVVAHREQREGSLQVGNEAP VTGSSPLGATQLD TDGALWLGGLPELPVGPALPKAYGT GFVGCLRDVVVGRHP LHLLEDAVTKPELRPCPTP

Gene Information

• Gene Name: AGRN agrin [ Homo sapiens ]
• Official Symbol: AGRN
• Synonyms: AGRN; agrin; AGRIN; agrin proteoglycan
• Gene ID: 375790
• mRNA Refseq: NM_198576
• Protein Refseq: NP_940978
• MIM: 103320
• Uniprot ID: O00468
• Chromosome Location: 1p36.33
• Pathway: Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; ECM-receptor interaction, organism-specific biosystem; ECM-receptor interaction, conserved biosystem; NCAM signaling for neurite out-growth, organism-specific biosystem
• Function: acetylcholine receptor regulator activity; laminin binding; protein binding; structural constituent of cytoskeleton

Reviews

11/23/2022

The packaging was secure, and the proteins maintained their integrity and activity upon arrival.

12/03/2021

I highly recommend their products for researchers seeking reliable reagents.

09/14/2021

They offer excellent value for the price.

07/09/2021

We rely on this protein for its consistent performance.

05/22/2021

I am extremely satisfied with the prompt delivery of the proteins.

Q&As

Can AGRN be used as a diagnostic marker for neurological diseases?

The measurement of AGRN levels or its isoforms in biological fluids or tissues may hold potential as diagnostic markers for certain neurological disorders, although further research is needed.

How is AGRN involved in neuromuscular junction formation?

AGRN interacts with specific receptors on the muscle and nerve cells, initiating a signaling cascade that leads to the clustering of acetylcholine receptors and the formation and maintenance of the NMJ.

Is AGRN involved in other organ systems besides the neuromuscular junction?

Yes, AGRN has been shown to have roles outside of the NMJ, including in the development of the central nervous system, kidneys, and cardiovascular system.

What are the isoforms of AGRN?

AGRN exists in multiple isoforms generated by alternative splicing. The most well-known isoforms include the neural isoforms (Agrin-N) and the muscle-specific isoforms (Agrin-M).

What is the structure of AGRN?

AGRN consists of several functional domains, including N-terminal laminin G-like (LG) domains, central EGF-like repeats, a C-terminal domain, and a heparin-binding site.

How is AGRN involved in cancer progression?

AGRN has been found to promote tumor cell migration, invasion, and metastasis by interacting with receptors and signaling pathways that regulate cell adhesion, migration, and angiogenesis.

How is AGRN expression regulated?

AGRN expression is tightly regulated during development and can be influenced by various signaling pathways, including Wnt, BMP, and Notch signaling.

What are the receptor molecules for AGRN?

AGRN interacts with two major receptors: muscle-specific kinase (MuSK) on the postsynaptic membrane and low-density lipoprotein receptor-related protein 4 (LRP4) on the presynaptic membrane.

How is AGRN involved in synaptic plasticity?

AGRN plays a role in regulating synaptic plasticity by modulating the structure and function of synapses, including their strength and stability.

How is AGRN involved in synaptic maturation?

AGRN regulates the maturation and stabilization of synapses by promoting the clustering of postsynaptic receptors and facilitating the development of pre- and postsynaptic specializations.