Recombinant Human AGRP, His-tagged

• Cat.No.: AGRP-3650H
• Product Overview: Total 128 AA. MW: 14.4 kDa (calculated). N-Terminal His-tag, 16 extra AA (highlighted).

Specification

• Cat. No.: AGRP-3650H
• Description: Agouti-related protein also called Agouti-related peptide (AgRP) is a neuropeptide produced in the brain by the AgRP/NPY neuron. It is only synthesised in NPY containing cell bodies located in the ventromedial part of the arcuate nucleus in the hypothalamus.
• Source: E. coli
• Species: Human
• Form: Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 5mM TRIS, 25mM NaCl, pH 7.5.
• Purity: >95%
• Research topic: Energy metabolism and body weight regulation.
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: ELISA; Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein
• Sequences of amino acids: MKHHHHHHHM LVPRGSAQMG LAPMEGIRRP DQALLPELPG LGLRAPLKKT TAEQAEEDLL QEAQALAEVL DLQDREPRSS RRCVRLHESC LGQQVPCCDP CATCYCRFFN AFCYCRKLGT AMNPCSRT
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
• Pathways: Adipocytokine signaling pathway; Syndecan-3-mediated signaling events

Gene Information

• Gene Name: AGRP agouti related protein homolog (mouse) [ Homo sapiens ]
• Official Symbol: AGRP
• Synonyms: AGRP; agouti related protein homolog (mouse); ART; AGRT; ASIP2; Agrt; MGC118963; agouti-related protein; OTTHUMP00000174803
• Gene ID: 181
• mRNA Refseq: NM_001138
• Protein Refseq: NP_001129
• MIM: 602311
• UniProt ID: O00253
• Chromosome Location: 16q22
• Function: neuropeptide hormone activity; receptor binding

Reviews

09/21/2019

This company offers a wide range of recombinant proteins at competitive prices.

08/08/2019

The pricing structure for their proteins is transparent and reasonable.

07/04/2019

The customer support team was extremely helpful and responsive, providing detailed information about the products.

05/09/2018

We are exceedingly satisfied with the quality of this protein.

03/14/2018

The exceptional quality of the proteins makes it a great value for the cost.

Q&As

Can AGRP be targeted for the treatment of obesity?

AGRP and the melanocortin system are potential targets for developing anti-obesity therapies, and research is ongoing to develop selective agonists or antagonists to modulate AGRP signaling.

Is AGRP involved in other physiological processes besides appetite regulation?

AGRP is also implicated in the modulation of autonomic functions, such as blood pressure regulation and cardiovascular control.

What are the downstream signaling pathways activated by AGRP?

AGRP inhibits the signaling of melanocortin receptors, leading to reduced cAMP production and affecting intracellular pathways involved in appetite and energy regulation.

How is AGRP involved in the central regulation of energy balance?

AGRP acts as a key neuropeptide in the hypothalamus, integrating signals from peripheral tissues to regulate energy homeostasis, appetite, and body weight.

What is the relationship between AGRP and the melanocortin system?

AGRP functions as an antagonist to melanocortin receptors, counteracting the effects of α-melanocyte-stimulating hormone (α-MSH), which promotes satiety and energy expenditure.

Can AGRP interact with other neuropeptides in appetite regulation?

Yes, AGRP can interact with other neuropeptides, such as neuropeptide Y (NPY), and together they influence the central regulation of appetite and energy balance.

Can alterations in AGRP expression contribute to eating disorders?

Dysregulation of AGRP expression or activity has been suggested to contribute to the development of certain eating disorders, such as binge eating disorder or hyperphagia.

Can AGRP be used as a diagnostic marker for metabolic disorders?

While AGRP itself is not typically used as a diagnostic marker, studying its expression levels or genetic variations may provide insights into the risk and development of metabolic disorders.

How does AGRP regulate appetite?

AGRP acts on the melanocortin receptors in the brain, inhibiting their activation and resulting in an increase in appetite and a decrease in energy expenditure.

Can AGRP be used as a biomarker for monitoring the efficacy of weight loss interventions?

AGRP levels may have potential as a biomarker for monitoring the response to weight loss interventions, but further research is needed to establish its clinical utility.