Recombinant Human AKD1 293 Cell Lysate

• Cat.No.: AKD1-8935HCL

Specification

• Description: Antigen standard for adenylate kinase domain containing 1 (AKD1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: AKD1 adenylate kinase domain containing 1 [ Homo sapiens ]
• Official Symbol: AKD1
• Synonyms: AKD1; adenylate kinase domain containing 1; adenylate kinase domain containing 2 , AKD2, C6orf199, C6orf224, chromosome 6 open reading frame 199 , chromosome 6 open reading frame 224; dJ70A9.1; FLJ25791; FLJ42177; MGC26954; AKD2; C6orf199; C6orf224
• Gene ID: 401271
• UniProt ID: Q5TCS8
• Chromosome Location: 6q21

Reviews

10/23/2021

AKD1 protein offers outstanding technical support, instilling confidence that any hurdles I may encounter can be swiftly resolved.

10/23/2021

the AKAP9 protein's extraordinary quality, coupled with the manufacturer's exceptional technical support, caters perfectly to my research needs.

09/20/2016

Their dedication to providing excellent assistance reflects their commitment to their customers' success.

06/03/2015

With their prompt and expert guidance, I am confident that any challenges I face along the way will be efficiently addressed, enabling smooth progression in my research.

Q&As

Can AKD1 be used as a biomarker for any diseases?

The potential for AKD1 to serve as a biomarker for specific diseases has not been widely explored. As of now, there is limited information regarding AKD1 as a potential biomarker. Additional research is necessary to evaluate its diagnostic or prognostic value in various diseases and to establish its utility as a biomarker.

What cellular processes or pathways does AKD1 regulate?

The exact cellular processes or pathways regulated by AKD1 are currently not well understood. However, based on its putative role in DNA repair, it is likely involved in maintaining genomic stability and cell survival after DNA damage. Further studies are needed to determine the full range of cellular processes regulated by AKD1.

Does AKD1 have any relevance to aging or age-related diseases?

The role of AKD1 in age-related diseases and aging itself is not well understood. However, since DNA damage and impaired repair mechanisms play a role in aging and various age-related diseases, it is possible that AKD1 dysfunction may contribute to these processes. Further research is needed to explore this potential link.

How is the AKD1 protein regulated?

The regulation of AKD1 protein involves various mechanisms. It undergoes post-translational modifications such as phosphorylation, which can influence its activity. Additionally, its expression levels can be controlled by different factors, including DNA damage and cellular stress signals.

Is AKD1 conserved across different species?

The conservation of AKD1 across different species has not been extensively studied. However, preliminary analysis suggests that AKD1 is evolutionarily conserved, with homologous genes identified in various organisms. Further research is needed to determine the extent of conservation and functional similarity of AKD1 across different species.

What are the future directions of research on the AKD1 protein?

Future research on the AKD1 protein will likely focus on elucidating its exact molecular mechanisms of action, understanding its regulation in response to DNA damage, and exploring its potential as a therapeutic target. Additionally, studying AKD1 in the context of different disease conditions, including cancer, immunodeficiency, and aging, will be an important area of investigation.

Are there any known inhibitors or activators of the AKD1 protein?

At present, specific inhibitors or activators of the AKD1 protein have not been reported. The understanding of AKD1 regulation and its potential modulation is still in its early stages, and further research is necessary to identify potential compounds or molecules that can modulate AKD1 activity.

Can the AKD1 protein be targeted for therapeutic purposes?

There is ongoing research exploring the potential therapeutic targeting of the AKD1 protein. By modulating its activity or expression, it may be possible to enhance DNA repair mechanisms in cancer cells, making them more susceptible to treatments like radiotherapy or chemotherapy. However, more studies are needed to validate the effectiveness and safety of targeting AKD1 in a clinical setting.

How is AKD1 regulated or modulated in the cell?

The regulation or modulation of AKD1 in the cell is not fully understood. However, like many proteins, it is likely that AKD1 expression, activity, or stability is regulated by various mechanisms. These could include post-translational modifications, such as phosphorylation or ubiquitination, as well as transcriptional and translational control. Further research is needed to uncover the precise regulatory mechanisms governing AKD1 in cells.

Is AKD1 involved in any other cellular pathways apart from DNA repair?

While AKD1's function in DNA repair is the most well-studied aspect, it is possible that AKD1 is involved in other cellular pathways as well. However, these potential additional roles of AKD1 have not been extensively investigated, and more research is needed to identify and characterize any other pathways in which AKD1 may play a role.

Are there any known genetic mutations in the AKD1 gene?

Currently, there is limited information available about genetic mutations in the AKD1 gene. The exploration of AKD1 mutations and their association with diseases is an area of ongoing research that requires further investigation. Comprehensive genomic studies and population-wide sequencing projects may provide more insights into potential mutations in the AKD1 gene and their implications.

Are there any other proteins or molecules that interact with AKD1?

The specific protein interactions of AKD1 have not been extensively studied. However, it is possible that AKD1 interacts with other DNA repair proteins or components of the DNA damage response pathway. Further research is needed to identify and characterize these potential interacting partners.

Is there any ongoing research related to the AKD1 protein?

Yes, ongoing research focuses on further understanding the role of AKD1 in DNA repair and its potential implications in cancer development and treatment. Scientists are also investigating the mechanisms underlying AKD1 regulation and exploring possible therapeutic strategies targeting this protein.

Does AKD1 play a role in cancer development or progression?

The exact role of AKD1 in cancer development or progression is not well understood. However, some studies have suggested that AKD1 may be involved in cell survival and DNA repair processes, which could have implications for cancer biology. Further research is needed to determine whether AKD1 dysregulation contributes to cancer development or progression and the underlying mechanisms involved.

Are mutations in the AKD1 gene associated with any diseases?

Yes, mutations in the AKD1 gene can lead to immunodeficiency and radiosensitivity syndrome. Individuals with such mutations have impaired DNA repair capabilities, resulting in an increased susceptibility to infections and a higher risk of developing cancer.

Are there any diseases or conditions associated with AKD1 dysfunction?

The specific diseases or conditions associated with AKD1 dysfunction are currently unknown. However, since DNA repair defects are implicated in various diseases, including cancer and certain genetic disorders, it is possible that abnormalities in AKD1 function could contribute to the development or progression of these conditions. Further research is needed to explore the potential link between AKD1 dysfunction and specific diseases.

Are there any animal models used to study the AKD1 protein?

Yes, animal models such as mice have been used to study the AKD1 protein and its role in DNA repair. These models allow researchers to investigate the physiological consequences of AKD1 deficiency and gain insights into its function in various biological processes.

Are there any known interactions or partners of the AKD1 protein?

The AKD1 protein interacts with various other proteins involved in the DNA repair pathway. For example, it forms a complex with DNA-dependent protein kinase (DNA-PK) and XRCC4, contributing to the efficiency of NHEJ-mediated repair.

How was the AKD1 protein discovered?

The AKD1 protein was initially discovered through genomic sequencing and bioinformatics analysis. Database searches for genes with homology to known DNA repair proteins led to the identification of the AKD1 gene and subsequently its corresponding protein.

Can AKD1 be targeted for drug development?

The potential of AKD1 as a target for drug development is still uncertain. While findings from preclinical studies suggest that modulating AKD1 activity may have therapeutic benefits, further research is needed to validate its clinical significance and develop specific inhibitors or activators of AKD1. Drug development targeting AKD1 will require a more comprehensive understanding of its precise role and mechanism of action.