Recombinant Human ALDM, His-tagged

• Cat.No.: ALDM-3265H
• Product Overview: Aldehyde Dehydrogenase, Mitochondrial (ALDM)

Specification

• Description: This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Source: E. Coli
• Species: Human
• Tag: His
• Form: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Molecular Mass: 16.1kDa
• Protein length: 517
• Endotoxin: <1.0eu per 1ug (determined by the lal</1.0eu
• Purity: >95%
• Applications: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Notes: Small volumes of ALDM recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Warning: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Gene Information

• Gene Name: ALDH2 aldehyde dehydrogenase 2 family (mitochondrial) [ Homo sapiens ]
• Official Symbol: ALDM
• Synonyms: ALDM; ALDHI; ALDH-E2; aldehyde dehydrogenase, mitochondrial; ALDH class 2; liver mitochondrial ALDH; acetaldehyde dehydrogenase 2; nucleus-encoded mitochondrial aldehyde dehydrogenase 2
• Gene ID: 217
• mRNA Refseq: NM_000690
• Protein Refseq: NP_000681
• MIM: 100650
• UniProt ID: P05091
• Chromosome Location: 12q24.2
• Pathway: Arginine and proline metabolism, organism-specific biosystem; Fatty Acid Omega Oxidation, organism-specific biosystem
• Function: aldehyde dehydrogenase (NAD) activity; aldehyde dehydrogenase [NAD(P)+] activity

Reviews

02/27/2022

The bands of proteins in Western Blotting experiments were very clear.

10/24/2021

Researchers can rely on the ALDM Protein to deliver reliable and reproducible results, facilitating robust data interpretation and scientific advancements.

04/06/2020

Its impressive performance and diverse applications make it an invaluable tool for a wide range of research endeavors.

10/29/2018

It has been widely employed in protein electron microscopy structure analysis, where it has demonstrated remarkable efficacy.

Q&As

Can ALDM protein be used as a biomarker for certain diseases?

Yes, ALDM protein levels or activity can serve as biomarkers for certain diseases. For example, ALDH1 protein levels have been used as a possible biomarker for cancer stem cells in specific types of cancer.

Can ALDM protein levels be used to predict treatment response or prognosis in certain diseases?

In some cases, ALDM protein levels have been found to correlate with treatment response or prognosis in certain diseases. For example, high ALDH activity in cancer cells has been associated with chemotherapy resistance and poor prognosis. However, the usefulness of ALDM protein as a predictive biomarker may vary depending on the specific disease and context.

Are there different isoforms of the ALDM protein?

Yes, there are multiple isoforms of aldehyde dehydrogenase. Two well-studied isoforms in humans are referred to as ALDH1 and ALDH2.

Can ALDM protein be targeted for therapeutic purposes?

Yes, ALDM protein has been considered as a potential target for therapeutic interventions. Modulating its activity or expression may have implications in various conditions, including cancer treatment and neurodegenerative disorders. However, further research is needed to explore its therapeutic potential.

Is ALDM protein involved in any other biological processes?

Yes, aldehyde dehydrogenase is involved in various biological processes, such as the breakdown of certain neurotransmitters, retinol metabolism, and cellular defense against oxidative stress.

Are there any diseases or disorders associated with ALDM protein dysfunction?

ALDM protein dysfunction has been linked to various diseases and disorders, including alcohol-related liver diseases, neurodegenerative disorders, and certain types of cancer.

Is ALDM protein involved in the metabolism of endogenous aldehydes?

Yes, ALDM protein is involved in the metabolism of endogenous aldehydes, which are byproducts of various cellular processes, including lipid peroxidation and carbohydrate metabolism. By converting these reactive aldehydes into less toxic compounds, ALDM protein helps prevent cellular damage and maintain cellular homeostasis.

Is ALDM protein or ALDH activity associated with aging or age-related diseases?

There is evidence suggesting that ALDH activity decreases with age, which may contribute to age-related diseases and impairments, such as neurodegenerative disorders and cardiovascular diseases. ALDH activity has also been found to play a role in the pathogenesis of certain age-related conditions, such as Alzheimer's disease.

Are there any side effects or risks associated with modulating ALDM protein activity?

Modulating ALDM protein activity could potentially have side effects or risks, especially if not carefully addressed. Inhibiting ALDH activity, for example, can disrupt normal cellular functions and lead to adverse effects. Therefore, any therapeutic interventions targeting ALDM protein should be carefully designed and tested to minimize potential risks.

What are the consequences of ALDM protein deficiency or genetic variations?

ALDM protein deficiency or genetic variations can lead to impaired alcohol metabolism, resulting in alcohol intolerance or Asian Flush syndrome. It can also affect other metabolic processes involving aldehyde detoxification.

Is ALDM protein involved in retinoic acid signaling?

Yes, some isoforms of ALDM protein, like ALDH1A1, are involved in retinoic acid (a derivative of vitamin A) synthesis and signaling, which is important for embryonic development and cellular differentiation.

Are there any pharmacological inhibitors or activators of ALDM protein?

Yes, there are compounds that can either inhibit or activate ALDM protein activity. For instance, disulfiram is a drug that inhibits ALDH activity and is used as a deterrent for alcohol consumption. On the other hand, ALDH activators are being explored as potential therapeutics for certain conditions, such as cardiovascular diseases and neurodegenerative disorders.

Does ALDM protein play a role in oxidative stress protection?

Yes, ALDM protein has been shown to participate in cellular defense against oxidative stress by metabolizing reactive aldehydes generated during oxidative damage.

What are the substrates of ALDM protein?

The substrates of ALDM protein are aldehydes, which are organic compounds containing a carbonyl group (C=O).

Are there any known genetic diseases associated with mutations in the ALDM protein?

Yes, mutations in the ALDH gene that codes for ALDM protein can lead to various genetic diseases, such as Sjögren-Larsson syndrome and pyridoxine-dependent epilepsy.

Is ALDM protein involved in the detoxification of environmental toxins?

Yes, ALDM protein plays a crucial role in the detoxification of environmental toxins. It helps metabolize and detoxify a variety of exogenous compounds, including environmental pollutants, drugs, and alcohol. By converting reactive and toxic aldehydes into less harmful substances, ALDM protein contributes to the body's defense mechanisms against the harmful effects of environmental toxins.

How is the activity of ALDM protein regulated?

The activity of ALDM protein can be regulated through various mechanisms, including gene expression regulation, post-translational modifications, and interactions with other proteins or cofactors.

Are there any genetic mutations or polymorphisms in ALDM protein that are associated with disease susceptibility?

Some genetic mutations or polymorphisms in ALDM protein have been associated with disease susceptibility or risk. For instance, certain variants in ALDH enzymes have been linked to an increased risk of alcohol-related diseases, such as alcoholic liver disease. Additionally, genetic variations in ALDM protein have been implicated in the development of certain types of cancer, including breast and lung cancer.

Are there any drugs or therapies that specifically target ALDM protein for cancer treatment?

While there aren't currently any approved drugs that specifically target ALDM protein for cancer treatment, research is ongoing to develop ALDH inhibitors that could be used as targeted therapies. These inhibitors aim to disrupt the cancer stem cell population, which is believed to be responsible for tumor growth, metastasis, and resistance to traditional therapies.

Are there any natural compounds or dietary components that can modulate ALDM protein activity?

Yes, certain natural compounds or dietary components have been shown to modulate ALDM protein activity. For instance, certain phytochemicals, like curcumin found in turmeric and resveratrol found in red grapes, have been reported to enhance ALDH activity. Additionally, certain dietary components, such as cruciferous vegetables and garlic, have been associated with increased ALDH activity.

Are there any other functions or roles of ALDM protein in the body?

Yes, ALDM protein has been implicated in various other physiological processes and pathologies. It plays a role in the metabolism of neurotransmitters, such as dopamine and serotonin, and can influence their levels and signaling in the brain. ALDM protein has also been associated with the regulation of stem cell self-renewal and differentiation. Additionally, ALDH enzymes have been linked to drug resistance in cancer cells.

Can ALDM protein be used as a target for drug delivery systems?

ALDM protein has been explored as a potential target for drug delivery systems. Since ALDH enzymes are found in different tissues and certain diseases exhibit increased ALDH expression, targeting ALDH-positive cells or tissues could enhance drug delivery and improve therapeutic efficacy. This approach is still in the early stages of development and requires further research.