Recombinant Human ALKBH2 293 Cell Lysate
Cat.No. : | ALKBH2-8902HCL |
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Description : | Antigen standard for alkB, alkylation repair homolog 2 (E. coli) (ALKBH2), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | ALKBH2 alkB, alkylation repair homolog 2 (E. coli) [ Homo sapiens ] |
Official Symbol : | ALKBH2 |
Synonyms : | ALKBH2; alkB, alkylation repair homolog 2 (E. coli); alpha-ketoglutarate-dependent dioxygenase alkB homolog 2; ABH2; MGC90512; oxy DC1; 2OG-Fe(II) oxy DC1; alkylated DNA repair protein alkB homolog 2; FLJ99103; |
Gene ID : | 121642 |
mRNA Refseq : | NM_001001655 |
Protein Refseq : | NP_001001655 |
MIM : | 610602 |
UniProt ID : | Q6NS38 |
Chromosome Location : | 12q24.11 |
Pathway : | ABH2 mediated Reversal of Alkylation Damage, organism-specific biosystem; DNA Damage Reversal, organism-specific biosystem; DNA Repair, organism-specific biosystem; Reversal of Alkylation Damage By DNA Dioxygenases, organism-specific biosystem; |
Function : | DNA-N1-methyladenine dioxygenase activity; cytosine C-5 DNA demethylase activity; cytosine C-5 DNA demethylase activity; ferrous iron binding; metal ion binding; oxidoreductase activity; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; |
Products Types
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ALKBH2-475H | Recombinant Human ALKBH2 Protein, GST-tagged | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (18)
Ask a questionThe functional regulation of ALKBH2 may be affected by epigenetic changes, such as histone modification and DNA methylation.
Mutations in ALKBH2 may affect the development of embryos and lead to related diseases.
ALKBH2 is involved in biological processes such as double-stranded DNA repair and cell cycle control.
ALKBH2 plays an important role in the DNA demethylation pathway and is involved in a number of DNA repair related pathways.
ALKBH2 interacts with a series of DNA repair related proteins such as DNA methyltransferase and DNA repair activator to participate in DNA repair.
ALKBH2 gene contains 10 exons and its expression is influenced by several regulatory elements and signaling pathways.
The activity of ALKBH2 may be regulated and interfered with by some chemicals, but the specific mechanism of action needs further study.
It is mainly used to modify DNA, but it also has some ability to modify RNA.
Overexpression or knockout of ALKBH2 can lead to incomplete repair of DNA damage and affect cell proliferation and division.
ALKBH2 is mainly found in the nucleus and is involved in biological processes such as DNA repair.
ALKBH2 removes methyl groups from DNA bases and is involved in DNA repair and cell proliferation.
This protein plays an important role in a variety of tumors, such as colorectal cancer, small cell lung cancer and so on.
A number of inhibitors have been developed that target ALKBH2 and inhibit its ability to acquire DNA methylation repair.
It is involved in multiple DNA single and double strand damage repair pathways, including light damage repair pathways.
Current research data show that ALKBH2 is associated with gene transcription, but the specific mechanism of action remains to be explored.
Mutations in ALKBH2 have been linked to some DNA repair related diseases and cancers.
It plays an important role in several neurological diseases, such as neurodegenerative diseases and Parkinson's disease.
ALKBH2 is involved in biological processes such as DNA repair, cell proliferation and differentiation.
Customer Reviews (5)
Write a reviewExhibited the expected biological activities, such as enzymatic activity and receptor binding activity.
ALKBH2 is highly expressed and can efficiently synthesize a large number of target proteins in host cells.
Good crystallization properties when analyzed by X-ray crystallography or other crystallization techniques.
The products are reliable and stable, and are widely used in scientific research, industrial production and medical fields.
The purity of ALKBH2 was good and the results obtained after analysis by SDS-PAGE or Western blot were excellent.
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