Recombinant Human ALOX5 protein, His-tagged
Cat.No. : | ALOX5-6847H |
Product Overview : | Recombinant Human ALOX5 aa. (Glu461~Ser671 (Accession # P09917)) fused with N-terminal His tag was produced in E. coli cells. |
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Description : | This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
Source : | E. coli |
Species : | Human |
Tag : | His |
Form : | Freeze-dried powder |
Molecular Mass : | Predicted Molecular Mass: 25.9kDa. |
Protein length : | Glu461~Ser671 (Accession # P09917) |
Endotoxin : | <1.0EU per 1ug (determined by the LAL method) |
Purity : | >95% |
Characteristic : | The isoelectric point is 5.5. |
Applications : | SDS-PAGE; WB; ELISA; IP. |
Stability : | The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition. |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
Storage buffer : | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
Reconstitution : | Reconstitute in sterile PBS, pH7.2-pH7.4. |
Gene Name : | ALOX5 arachidonate 5-lipoxygenase [ Homo sapiens (human) ] |
Official Symbol : | ALOX5 |
Synonyms : | 5-LO; 5LPG; LOG5; 5-LOX; LOX-5; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic acid 5-lipoxygenase; leukotriene A4 synthase; arachidonate 5-lipoxygenase |
Gene ID : | 240 |
mRNA Refseq : | NM_000698.4 |
Protein Refseq : | NP_000689.1 |
UniProt ID : | P09917 |
Products Types
◆ Recombinant Protein | ||
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ALOX5-294R | Recombinant Rat ALOX5 Protein, His (Fc)-Avi-tagged | +Inquiry |
ALOX5-053H | Recombinant Human ALOX5 Protein | +Inquiry |
Alox5-583M | Recombinant Mouse Alox5 Protein, MYC/DDK-tagged | +Inquiry |
ALOX5-483M | Recombinant Mouse ALOX5 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
ALOX5-8896HCL | Recombinant Human ALOX5 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (13)
Ask a questionThe ALOX5 coding gene is located on human chromosome 10.
Currently known ALOX5 inhibitors include trypanosomamine, Ritopane, MK-886, etc., whose mechanism of action is to interfere with the expression or function of ALOX5 in different ways, thereby playing an anti-inflammatory or anti-tumor role.
ALOX5 is involved in a variety of biological processes, including apoptosis, cell proliferation, inflammatory response, and cancer metastasis.
The gene expression can be regulated by a variety of factors such as prostaglandins, cytokines and interferons.
Some foods rich in polyphenols, such as cucumbers, tomatoes, grapes, green tea, etc., are thought to inhibit ALOX5 activity.
In general, the ALOX5 gene is highly expressed in eosinophils and monocytes.
The ALOX5 promoter region is the starting point of gene transcription and is responsible for mediating the transcription regulation of the ALOX5 gene.
Drugs that block the ALOX5 signaling pathway, such as Zileuton, MK886, and A861, have been shown to inhibit tumor cell proliferation and invasion. Currently, there are also many researchers who are exploring ALOX5 in tumors.
ALOX5 gene mutation may cause asthma, allergic rhinitis, eczema and other allergic diseases.
5-HETE is one of the representative products of ALOX5 catalyzed synthesis, and is an activator of platelets and neutrophils.
There is no definite answer to this question. Current studies believe that ALOX5 can affect the occurrence, development and metastasis of tumors through multiple ways, including regulating the proliferation, apoptosis, invasion and metastasis of tumor cells.
ALOX5 knockout mouse models can be used to study a variety of biological processes and diseases, such as immune regulation, inflammation, asthma, eczema, autoimmunity, etc.
At present, the commonly used methods include real-time fluorescent quantitative PCR, Northern blot, Western blot, etc.
Customer Reviews (3)
Write a reviewAfter ALOX5 application, the background of the Western blot was very clean with no nonspecific signal.
ALOX5 significantly improved the experimental accuracy and made the experimental results more reliable.
I appreciate the stability of ALOX5, which can be kept at room temperature for several days without spoilage.
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