Recombinant Human ALPK1 protein, His-tagged

• Cat.No.: ALPK1-3097H
• Product Overview: Recombinant Human ALPK1 protein(1-347 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 1-347 aa
• AA Sequence: MNNQKVVAVLLQECKQVLDQLLLEA PDVSEEDKSEDQRCRALLPSELRTL IQEAKEMKWPFVPEKWQYKQAVGPE DKTNLKDVIGAGLQQLLASLRASIL ARDCAAAAAIVFLVDRFLYGLDVSG KLLQVAKGLHKLQPATPIAPQVVIR QARISVNSGKLLKAEYILSSLISNN GATGTWLYRNESDKVLVQSVCIQIR GQILQKLGMWYEAAELIWASIVGYL ALPQPDKKGLSTSLGILADIFVSMS KNDYEKFKNNPQINLSLLKEFDHHL LSAAEACKLAAAFSAYTPLFVLTAV NIRGTCLLSYSSSNDCPPELKNLHL CEAKEAFEIGLLTKRDDEPVTG
• Purity: 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: ALPK1 alpha-kinase 1 [ Homo sapiens ]
• Official Symbol: ALPK1
• Synonyms: ALPK1; alpha-kinase 1; alpha-protein kinase 1; FLJ22670; KIAA1527; Lak; lymphocyte alpha kinase; chromosome 4 kinase; lymphocyte alpha-kinase; lymphocyte alpha-protein kinase; LAK; 8430410J10Rik
• Gene ID: 80216
• mRNA Refseq: NM_001102406
• Protein Refseq: NP_001095876
• MIM: 607347
• UniProt ID: Q96QP1

Reviews

03/26/2022

Highly effective fusion protein detection.

06/16/2019

Exceptional protein-protein interaction analysis.

02/09/2017

Specific target detection in immunohistochemistry.

Q&As

Can ALPK1 kinase activity be modulated by specific small molecules or inhibitors?

Yes, ALPK1 kinase activity can be modulated by specific small molecule inhibitors that target its ATP-binding site.

How does ALPK1 influence intracellular signaling cascades or gene expression patterns?

ALPK1 influences intracellular signaling cascades such as MAPK and AKT pathways and can modulate the expression of specific target genes.

What is the impact of ALPK1 knockout or overexpression on cellular phenotypes?

ALPK1 knockout or overexpression can lead to altered cell morphology, proliferation rates, and cell cycle progression.

What is the subcellular localization of ALPK1 in human cells?

ALPK1 is primarily localized in the cytoplasm of human cells.

Is ALPK1 involved in the regulation of cellular apoptosis or survival pathways?

ALPK1 has been associated with both pro-apoptotic and anti-apoptotic signaling pathways, suggesting a complex role in cellular survival.

Does ALPK1 expression change under specific cellular stress conditions?

Yes, the expression of ALPK1 can be upregulated or downregulated in response to various stress stimuli.

How does ALPK1 contribute to cellular proliferation and growth?

ALPK1 is implicated in promoting cellular proliferation and growth.

Does ALPK1 play a role in cellular migration or invasion processes?

ALPK1 has been implicated in regulating cellular migration and invasion in certain cell types.

Are there any specific post-translational modifications that regulate ALPK1 activity?

Phosphorylation is a known post-translational modification that can regulate ALPK1 activity.

Are there any known interacting proteins or signaling pathways associated with ALPK1?

Several interacting proteins and signaling pathways have been identified, including protein kinases, phosphatases, and cytoskeletal regulators.