Recombinant Human AMIGO1 protein, His & T7-tagged

• Cat.No.: AMIGO1-1561H
• Product Overview: Recombinant Human AMIGO1 aa. (Gly28~Thr372) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 43.0kDa
• Protein length: Gly28~Thr372
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Applications: WB, ICC, IHC-P, IHC-F, ELISA.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Store at 4 oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
• Concentration: 200μg/mL
• Storage buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: AMIGO1 adhesion molecule with Ig like domain 1 [ Homo sapiens (human) ]
• Official Symbol: AMIGO1
• Synonyms: AMIGO1; adhesion molecule with Ig like domain 1; ALI2; AMIGO; AMIGO-1; amphoterin-induced protein 1; alivin-2; amphoterin-induced gene and ORF
• Gene ID: 57463
• mRNA Refseq: NM_020703.3
• Protein Refseq: NP_065754.2
• UniProt ID: Q86WK6

Reviews

11/12/2022

Sensitivity in detecting low-abundance proteins.

08/14/2022

Specific target detection in various tissue types.

04/23/2022

Robust assay performance in high-throughput screening.

Q&As

What is the role of AMIGO1 in neurite outgrowth and axon guidance?

AMIGO1 plays a critical role in promoting neurite outgrowth and axon guidance by regulating cytoskeletal dynamics and signaling pathways involved in axonal growth cone guidance.

What are the molecular mechanisms underlying the interaction between AMIGO1 and its binding partners?

AMIGO1 interacts with various binding partners, including cell adhesion molecules and ion channels, through both homophilic and heterophilic interactions, contributing to neuronal cell-cell communication and synaptic connectivity.

How does AMIGO1 localization change during neuronal development and maturation?

During neuronal development, AMIGO1 undergoes dynamic changes in its subcellular localization, transitioning from a primarily intracellular distribution to a more plasma membrane-associated localization.

Can AMIGO1 regulate neuronal migration during brain development?

AMIGO1 has been implicated in the regulation of neuronal migration during brain development, particularly in the formation of cortical layers and the establishment of neuronal circuitry.

How does AMIGO1 influence neuronal survival and apoptosis?

AMIGO1 exerts a neuroprotective effect by promoting neuronal survival and inhibiting apoptosis through the activation of intracellular signaling pathways that regulate cell survival and anti-apoptotic factors.

What is the impact of AMIGO1 on myelination and oligodendrocyte development?

AMIGO1 is involved in myelination processes and oligodendrocyte development, promoting the differentiation and maturation of oligodendrocytes and facilitating proper myelin sheath formation.

What is the tissue-specific expression pattern of AMIGO1 in different organs?

AMIGO1 shows a distinct tissue-specific expression pattern, with high expression in the brain, particularly in the developing and mature neurons, as well as in other organs such as the heart, kidney, and lung.

Does AMIGO1 modulate synaptic plasticity and neurotransmission?

AMIGO1 has been shown to modulate synaptic plasticity by regulating the surface expression of neurotransmitter receptors and modulating synaptic strength and connectivity.

Is there a correlation between AMIGO1 expression and neurological disorders?

Altered expression or dysregulation of AMIGO1 has been associated with various neurological disorders, including epilepsy, schizophrenia, and autism spectrum disorders, suggesting its potential involvement in disease pathogenesis.

Can modulation of AMIGO1 expression or activity be a potential therapeutic approach for neurological conditions?

Modulation of AMIGO1 expression or activity represents a potential therapeutic target for neurological conditions, and further studies investigating the specific mechanisms and downstream signaling pathways regulated by AMIGO1 may unveil novel therapeutic strategies for these disorders.