Recombinant Human AMIGO3 293 Cell Lysate
Cat.No. : | AMIGO3-8881HCL |
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Description : | Antigen standard for adhesion molecule with Ig-like domain 3 (AMIGO3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | AMIGO3 adhesion molecule with Ig-like domain 3 [ Homo sapiens ] |
Official Symbol : | AMIGO3 |
Synonyms : | AMIGO3; adhesion molecule with Ig-like domain 3; amphoterin-induced protein 3; amphoterin induced gene and open reading frame 3; alivin-3; amphoterin-induced gene and ORF 3; AMIGO-3; MGC120552; |
Gene ID : | 386724 |
mRNA Refseq : | NM_198722 |
Protein Refseq : | NP_942015 |
UniProt ID : | Q86WK7 |
Chromosome Location : | 3p21 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionAMIGO3 exhibits a unique tissue-specific expression pattern, with prominent expression in the central nervous system, particularly in regions involved in neuronal development and synaptic transmission, and lower expression in other organs.
Modulation of AMIGO3 expression or activity has the potential to influence neuronal plasticity and regeneration, promoting axonal growth and functional recovery in the context of injury or neurodegenerative conditions.
AMIGO3 may play a role in the formation and maintenance of myelin sheaths in the peripheral nervous system, contributing to proper nerve conduction and insulation of axons.
The regulation of AMIGO3 expression during brain development and in response to environmental stimuli involves complex molecular mechanisms, including transcriptional regulation, epigenetic modifications, and signaling pathways that respond to cues from the local microenvironment.
Different isoforms of AMIGO3 may have distinct functions, potentially influencing cellular processes such as neurite outgrowth, synaptogenesis, and cell adhesion in a context-dependent manner.
Targeting AMIGO3 expression or activity may hold promise as a therapeutic strategy for neurological conditions or neurodegenerative diseases, and further research is needed to understand its precise role and potential therapeutic implications.
Knockout or overexpression studies of AMIGO3 have revealed its critical role in neuronal connectivity and synaptic function, affecting processes such as dendritic arborization, synapse formation, and neurotransmitter release.
AMIGO3 interacts with specific cell adhesion molecules and signaling receptors, including members of the immunoglobulin superfamily and receptor tyrosine kinases, activating downstream signaling pathways involved in axonal guidance, cytoskeletal remodeling, and synaptic plasticity.
AMIGO3 plays a crucial role in neural development and axonal outgrowth, promoting the extension and guidance of growing axons during neuronal circuit formation.
Genetic variations in the AMIGO3 gene have been associated with neurological disorders, cognitive traits, and susceptibility to certain psychiatric conditions, suggesting a role in disease susceptibility and cognitive function.
Customer Reviews (3)
Write a reviewConsistent and reliable results across different experimental conditions.
High-resolution imaging for detailed protein localization.
Precise measurement of protein activity and function.
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