Recombinant Human AMN1 293 Cell Lysate
Cat.No. : | AMN1-8879HCL |
- Specification
- Gene Information
- Related Products
- Download
Description : | Antigen standard for antagonist of mitotic exit network 1 homolog (S. cerevisiae) (AMN1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | AMN1 antagonist of mitotic exit network 1 homolog (S. cerevisiae) [ Homo sapiens ] |
Official Symbol : | AMN1 |
Synonyms : | AMN1; antagonist of mitotic exit network 1 homolog (S. cerevisiae); protein AMN1 homolog; |
Gene ID : | 196394 |
mRNA Refseq : | NM_001113402 |
Protein Refseq : | NP_001106873 |
UniProt ID : | Q8IY45 |
Chromosome Location : | 12p11.21 |
Products Types
◆ Recombinant Protein | ||
AMN1-311R | Recombinant Rat AMN1 Protein, His (Fc)-Avi-tagged | +Inquiry |
AMN1-655R | Recombinant Rat AMN1 Protein | +Inquiry |
AMN1-527H | Recombinant Human AMN1 protein, GST-tagged | +Inquiry |
AMN1-4399Z | Recombinant Zebrafish AMN1 | +Inquiry |
AMN1-9621H | Recombinant Human AMN1, GST-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (19)
Ask a questionSeveral interactors and regulators of the AMN1 protein have been identified through experimental studies and protein-protein interaction databases. These include other ribosomal proteins, chaperones, and factors involved in ribosome biogenesis and translation.
The AMN1 protein is primarily associated with ribosome biogenesis and translation initiation, which are essential for protein synthesis. Dysregulation of these processes can impact cell growth, development, and disease progression.
Yes, the AMN1 protein is known to interact with other ribosomal proteins, such as Rps3p and Rps21p, to regulate ribosome assembly and function.
The AMN1 protein is primarily localized to the nucleus, where it plays a role in ribosome biogenesis. However, it may also localize to other cellular compartments, such as the nucleolus and cytoplasm, during certain cellular processes or under specific conditions.
The expression of the AMN1 gene can be regulated by various factors, including environmental signals, cellular processes, and transcription factors. However, the specific regulatory mechanisms of AMN1 gene expression are not fully understood.
While there are no known diseases directly linked to AMN1 protein mutations, dysregulation of ribosome biogenesis and translation processes can have broad impacts on cell growth, development, and disease progression. Further research may provide insights into potential disease associations.
Currently, there is limited information available regarding post-translational modifications of the AMN1 protein. Further research is required to identify and characterize potential modifications such as phosphorylation or ubiquitination.
Future studies can focus on elucidating the structural properties of AMN1 and its exact mechanisms in ribosome biogenesis and translation regulation. Additionally, investigating potential gene regulatory networks involving AMN1 could provide further insights into its biological functions.
The AMN1 protein is evolutionarily conserved, meaning that it is found in various species and shares certain structural and functional features across those species.
Currently, there are no known genetic disorders or diseases associated with AMN1 gene mutations in humans.
While research on the AMN1 protein is relatively limited, ongoing studies continue to investigate its role in ribosome biogenesis and its potential implications in cellular processes.
The AMN1 protein is primarily localized in the cytoplasm of cells.
Currently, there are no known specific mouse models or knockout studies targeting the AMN1 gene. However, studies involving other ribosomal proteins or factors related to ribosome biogenesis may indirectly provide insights into the function of the AMN1 gene.
While the primary known function of the AMN1 protein is in ribosome biogenesis, it may also have additional roles in cellular processes such as mRNA translation, stress responses, and cell cycle regulation. Further research is needed to uncover these potential functions.
The AMN1 protein is mainly associated with ribosome biogenesis and translation initiation. It plays a role in the assembly and maturation of ribosomes, which are essential for protein synthesis.
Yes, the AMN1 protein can interact with other proteins as part of ribosome assembly and translation initiation processes. It has been shown to physically interact with various ribosomal proteins, assembly factors, and translation initiation factors.
Yes, AMN1 protein orthologs have been identified in various eukaryotic organisms, including humans, mice, flies, and plants.
Genetic variants and mutations in the AMN1 gene have been identified in studies and databases. These variants can range from single nucleotide polymorphisms (SNPs) to larger structural variations. Some of these variants may affect the expression or function of the AMN1 protein.
The expression of the AMN1 gene has been detected in multiple tissues and cell types, suggesting a widespread expression pattern. However, the levels of AMN1 expression may vary across tissues, indicating some degree of tissue specificity.
Customer Reviews (4)
Write a reviewThe prompt and knowledgeable assistance they offer has greatly enhanced my confidence in using the AMN1 protein.
Its purity and reliability have provided consistent and reliable results in my research.
I am highly satisfied with the performance and support associated with this protein, as it has proven to be an essential tool in advancing my scientific investigations.
The AMN1 protein is of exceptional quality, making it a perfect fit for my experimental needs.
Ask a Question for All AMN1 Products
Required fields are marked with *
My Review for All AMN1 Products
Required fields are marked with *
Inquiry Basket