Recombinant Human AMY1B 293 Cell Lysate

• Cat.No.: AMY1B-8874HCL

Specification

• Description: Antigen standard for amylase, alpha 1B (salivary) (AMY1B) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: AMY1B amylase, alpha 1B (salivary) [ Homo sapiens ]
• Official Symbol: AMY1B
• Synonyms: AMY1B; amylase, alpha 1B (salivary); AMY1, amylase, alpha 1B; salivary; alpha-amylase 1; glycogenase; salivary alpha-amylase; salivary amylase alpha 1B; amylase, salivary, alpha-1B; 1,4-alpha-D-glucan glucanohydrolase 1; AMY1; AMY1A; AMY1C; MGC177995
• Gene ID: 277
• mRNA Refseq: NM_001008218
• Protein Refseq: NP_001008219
• MIM: 104701
• UniProt ID: P04745
• Chromosome Location: 1p21
• Pathway: Carbohydrate digestion and absorption, organism-specific biosystem; Carbohydrate digestion and absorption, conserved biosystem; Digestion of dietary carbohydrate, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of carbohydrates, organism-specific biosystem; Salivary secretion, organism-specific biosystem
• Function: catalytic activity; cation binding

Reviews

03/13/2023

AMY1B protein is an enzyme involved in the digestion of carbohydrates, specifically starch.

11/22/2020

A reliable manufacturer ensures the production and purification of AMY1B protein, maintaining its quality and purity.

08/16/2020

Such support helps researchers overcome challenges and obtain reliable results.

05/10/2019

Manufacturers might offer customization options, such as different variants or modifications of the AMY1B protein, to cater to specific research requirements.

01/11/2019

Manufacturers should provide detailed information about the production process, quality control measures, and purity assessment methods employed.

Q&As

Is there any association between the AMY1B gene and obesity?

The role of AMY1B gene variations in obesity or weight regulation has not been extensively studied. Most of the existing research on this topic has focused on the AMY1A gene and its association with obesity risk. Therefore, it is unclear if and how AMY1B gene variations may be related to obesity. Further research is needed to explore this potential association.

Can the expression or activity of the AMY1B gene be influenced by certain factors?

Similar to AMY1A, the expression and activity of the AMY1B gene may be influenced by various factors. This can include dietary factors such as carbohydrate intake, genetic factors, and environmental factors. More research is needed to fully understand the regulation and potential influences on AMY1B gene expression and activity.

Can variations in the AMY1B gene affect starch digestion efficiency?

As mentioned earlier, the AMY1B gene has been less studied compared to AMY1A. While variations or mutations in the AMY1A gene are known to influence starch digestion efficiency, there is limited information on the specific impact of AMY1B gene variations on this process. Further research is needed to determine if and how AMY1B gene variations might affect starch digestion efficiency.

Are there any diseases or disorders associated with the AMY1B protein?

Currently, there are no known diseases or disorders specifically associated with the AMY1B protein. However, it is important to note that research on this protein is relatively limited compared to AMY1A, so there may be more to discover in the future.

How does the AMY1B protein differ from the AMY1A protein?

The AMY1B protein and the AMY1A protein are two isoforms of the α-amylase protein. They are encoded by different genes, AMY1B and AMY1A, respectively. While both proteins are involved in carbohydrate digestion, they may have slight differences in their enzymatic activity or expression patterns. However, further research is needed to fully understand the functional distinctions between these two isoforms.

Does the AMY1B gene have heritability?

The AMY1B gene, like other genes, is heritable. It is passed down from parents to offspring in a Mendelian manner. However, the extent of gene copy number variation for AMY1B and its heritability patterns are not as well-studied as AMY1A. It is important to consider genetic and environmental factors that may influence AMY1B gene expression and activity.

Is the AMY1B gene expressed in all tissues of the body?

The expression pattern of the AMY1B gene in different tissues has not been extensively studied. However, it is generally believed that the AMY1B gene is primarily expressed in salivary glands, similar to the AMY1A gene. This is because both genes code for enzymes involved in starch digestion, and salivary glands are one of the major sites where α-amylase is produced.

Are there any genetic mutations or variations in the AMY1B gene?

The AMY1B gene is relatively less studied compared to AMY1A, and as a result, less is known about genetic mutations or variations specifically related to AMY1B. Further research is needed to fully understand the genetic variability and potential impact of AMY1B gene variations.

Are there any known genetic disorders associated with the AMY1B gene?

Currently, there are no known genetic disorders specifically associated with the AMY1B gene. However, it is important to note that research on this gene is relatively limited, and new discoveries may be made in the future that shed light on potential disorders or conditions related to AMY1B.