Recombinant Human ANKRD13D 293 Cell Lysate

• Cat.No.: ANKRD13D-8856HCL

Specification

• Description: Antigen standard for ankyrin repeat domain 13 family, member D (ANKRD13D), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ANKRD13D ankyrin repeat domain 13 family, member D [ Homo sapiens ]
• Official Symbol: ANKRD13D
• Synonyms: ANKRD13D; ankyrin repeat domain 13 family, member D; ankyrin repeat domain-containing protein 13D; MGC50828
• Gene ID: 338692
• mRNA Refseq: NM_207354
• Protein Refseq: NP_997237
• UniProt ID: Q6ZTN6
• Chromosome Location: 11q13.2

Reviews

10/26/2021

the ANKRD13D protein's quality and suitability for experimental needs, combined with the manufacturer's excellent technical support, create a valuable partnership that enhances research outcomes.

02/20/2020

This level of support saves researchers time and resources, enabling them to focus on the scientific aspects of their work and accelerate the overall progress of their studies.

07/21/2019

The manufacturer's commitment to excellence is evident through their provision of excellent technical support, which can prove vital in troubleshooting any experimental challenges or addressing specific research questions related to the protein.

02/10/2017

Their expertise can help troubleshoot problems, suggest alternative approaches, or provide recommendations for optimizing experimental protocols.

07/13/2016

Researchers can feel confident in the reliability and efficacy of the ANKRD13D protein and rely on the manufacturer's expertise to navigate any challenges that may arise during their trials.

Q&As

Is ANKRD13D differentially expressed in specific tissues or developmental stages?

The tissue-specific expression and developmental regulation of ANKRD13D have not been extensively characterized. Further studies, such as gene expression profiling, could provide insights into its expression patterns.

Is ANKRD13D post-translationally modified?

The post-translational modifications of ANKRD13D have not been well-studied. It is possible that it undergoes various modifications such as phosphorylation, ubiquitination, or acetylation, but further research is needed to understand its post-translational modification profile.

Are there any known functional domains or motifs in ANKRD13D?

Currently, there are no known functional domains or motifs identified in ANKRD13D. Further investigations and bioinformatic analyses may provide insights into its potential functional regions.

Are there any known interaction partners or binding partners of ANKRD13D?

The interaction partners or binding partners of ANKRD13D have not been characterized. Further studies such as protein-protein interaction assays may provide insights into its potential molecular interactions.

Has ANKRD13D been linked to any signaling pathways?

There are currently no established links between ANKRD13D and specific signaling pathways. Further studies, such as protein-protein interaction network analyses, may reveal its potential involvement in signaling cascades.

Has ANKRD13D been implicated in any cellular processes?

Given the lack of functional characterization, specific cellular processes in which ANKRD13D may be involved are still unknown. Further research is needed to determine its potential roles in cellular activities.

Are there any genetic variations or mutations associated with ANKRD13D?

The presence of genetic variations or mutations specifically associated with ANKRD13D has not been well-documented. Future studies, such as genetic association studies, could uncover any potential disease-related variants.

Are there any known orthologs or paralogs of ANKRD13D?

The presence of orthologs or paralogs of ANKRD13D across species has not been extensively investigated. Comparative genomics and phylogenetic analyses could shed light on its evolutionary relationships.