Recombinant Human ANXA10 293 Cell Lysate

• Cat.No.: ANXA10-8838HCL

Specification

• Description: Antigen standard for annexin A10 (ANXA10) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ANXA10 annexin A10 [ Homo sapiens ]
• Official Symbol: ANXA10
• Synonyms: ANXA10; annexin A10; ANX14; annexin 14; annexin-10; annexin-14
• Gene ID: 11199
• mRNA Refseq: NM_007193
• Protein Refseq: NP_009124
• MIM: 608008
• UniProt ID: Q9UJ72
• Chromosome Location: 4q32.3
• Function: calcium ion binding; calcium-dependent phospholipid binding

Reviews

07/23/2021

The outstanding level of customer service adds extra reassurance and confidence in using the ANXA10 protein for my scientific investigations.

05/25/2021

The ANXA10 protein surpasses expectations in terms of quality and will undoubtedly fulfill my experimental requirements.

07/26/2017

With the ANXA10 protein and its accompanying technical assistance, I am confident that I can achieve successful outcomes for my research endeavors.

05/11/2016

the manufacturer of ANXA10 provides exceptional technical support, offering valuable assistance in troubleshooting and experimental design.

Q&As

Is ANXA10 protein localized in a specific subcellular compartment?

ANXA10 protein is primarily known to localize to the cytoplasmic compartment of cells. It can also associate with cell membranes in some instances. However, the subcellular localization of ANXA10 may be cell type-specific and can vary depending on different physiological conditions.

Is ANXA10 protein involved in calcium signaling?

Annexin proteins, including ANXA10, have been shown to interact with calcium ions and are involved in calcium signaling pathways. They can regulate calcium fluxes across membranes and participate in calcium-mediated processes such as neurotransmitter release, muscle contraction, and cell migration. While there is limited information specifically regarding ANXA10's role in calcium signaling, it is possible that it may contribute to these processes.

Is ANXA10 protein expressed in all tissues?

The expression of ANXA10 protein has been reported to vary across different tissues. It appears to be more abundant in certain tissues, such as the liver and pancreas, while its expression in other tissues may be lower or undetectable.