Recombinant Human AP1G1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: AP1G1-1148H
• Product Overview: AP1G1 MS Standard C13 and N15-labeled recombinant protein (NP_001025178) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family. Two transcript variants encoding different isoforms have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 91.5 kDa
• AA Sequence: MPAPIRLRELIRTIRTARTQAEERE MIQKECAAIRSSFREEDNTYRCRNV AKLLYMHMLGYPAHFGQLECFKLIA SQKFTDKRIGYLGAMLLLDERQDVH LLMTNCIKNDLNHSTQFVQGLALCT LGCMGSSEMCRDLAGEVEKLLKTSN SYLRKKAALCAVHVIRKVPELMEMF LPATKNLLNEKNHGVLHTSVVLLTE MCERSPDMLAHFRKNEKLVPQLVRI LKNLIMSGYSPEHDVSGISDPFLQV RILRLLRILGRNDDDSSEAMNDILA QVATNTETSKNVGNAILYETVLTIM DIKSESGLRVLAINILGRFLLNNDK NIRYVALTSLLKTVQTDHNAVQRHR STIVDCLKDLDVSIKRRAMELSFAL VNGNNIRGMMKELLYFLDSCEPEFK ADCASGIFLAAEKYAPSKRWHIDTI MRVLTTAGSYVRDDAVPNLIQLITN SVEMHAYTVQRLYKAILGDYSQQPL VQVAAWCIGEYGDLLVSGQCEEEEP IQVTEDEVLDILESVLISNMSTSVT RGYALTAIMKLSTRFTCTVNRIKKV VSIYGSSIDVELQQRAVEYNALFKK YDHMRSALLERMPVMEKVTTNGPTE IVQTNGETEPAPLETKPPPSGPQPT SQANDLLDLLGGNDITPVIPTAPTS KPSSAGGELLDLLGDINLTGAPAAA PAPASVPQISQPPFLLDGLSSQPLF NDIAAGIPSITAYSKNGLKIEFTFE RSNTNPSVTVITIQASNSTELDMTD FVFQAAVPKTFQLQLLSPSSSIVPA FNTGTITQVIKVLNPQKQQLRMRIK LTYNHKGSAMQDLAEVNNFPPQSWQ TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: AP1G1 adaptor related protein complex 1 subunit gamma 1 [ Homo sapiens (human) ]
• Official Symbol: AP1G1
• Synonyms: AP1G1; adaptor-related protein complex 1, gamma 1 subunit; ADTG, CLAPG1; AP-1 complex subunit gamma-1; gamma1 adaptin; gamma adaptin; gamma1-adaptin; golgi adaptor HA1/AP1 adaptin gamma subunit; adaptor protein complex AP-1 subunit gamma-1; golgi adaptor HA1/AP1 adaptin subunit gamma-1; adapter-related protein complex 1 subunit gamma-1; clathrin assembly protein complex 1 gamma large chain; clathrin assembly protein complex 1 gamma-1 large chain; clathrin-associated/assembly/adaptor protein, large, gamma 1; ADTG; CLAPG1; MGC18255
• Gene ID: 164
• mRNA Refseq: NM_001030007
• Protein Refseq: NP_001025178
• MIM: 603533
• UniProt ID: O43747

Reviews

03/06/2022

The short half-life and high clearance of AP1G1 enable it to penetrate the tissue better, reach the lesion site, and improve the therapeutic effect.

01/21/2022

Production process of this recombinant protein is very environmentally friendly, from the selection of raw materials to the production process reflects the concept of green production.

10/12/2020

I appreciate the quality of this recombinant protein manufacturer's attention to the product, and their product stability is very good, which makes our experiment more smooth.

09/18/2020

The strip shape of AP1G1 was regular in Western blot, showing good quality.

Q&As

What are the important applications of AP1G1 in scientific research?

AP1G1 can be used to study the mechanism of tumor occurrence and development, immune regulation, protein structure and function, and has important scientific application value.

What is the action mechanism of AP1G1 ?

Recombinant AP1G1 regulates signal transduction and metabolic pathways of target cells primarily by binding to specific receptors, and is involved in a range of biological processes.

Which application prospect of AP1G1 in cancer therapy?

AP1G1 has a certain application prospect in cancer therapy, it can participate in the regulation of tumor cell proliferation, apoptosis and other processes, and may be used in the development of new drugs.

Does the stability of AP1G1 ?

The AP1G1 is highly stable and can maintain its biological activity and effect under different conditions.

What is the role of AP1G1 in the treatment of diabetes?

Recombinant AP1G1 is involved in the regulation of insulin secretion and glucose metabolism, so it has certain application prospects in the treatment of diabetes.

What is the application status of recombinant AP1G1 in clinical trials?

The recombinant AP1G1 protein has made some progress in clinical trials, but its application still needs more research and evaluation.

How the clinical application of AP1G1 ?

AP1G1 can be used to treat diseases such as tumors, diabetes, autoimmune diseases, and can also be used for research in the field of biotechnology.

What structural characteristics of AP1G1 ?

The structure of the AP1G1 is characterized by a complex of multiple protein subunits that form a stable three-dimensional structure through specific interactions.

What are the pharmacokinetic characteristics of AP1G1 ?

The absorption, metabolism and excretion characteristics of AP1G1 in vivo still need to be further studied and analyzed.

What is the synthesis method of AP1G1 ?

The synthesis of AP1G1 can be completed by cloning, expression, purification and structural analysis of foreign genes.

What is the application prospect of recombinant AP1G1 in the field of biotechnology?

AP1G1 can be used as an important biomolecular tool to study protein structure and function, and has broad application prospects.

What is the impact of the difference between recombinant AP1G1 and natural AP1G1?

Differences between the recombinant AP1G1 protein and the natural AP1G1 protein may affect its biological activity and effect, and further experimental and clinical testing is needed to evaluate it.

How effective is recombinant AP1G1 compared to other treatments?

The effects of AP1G1 compared to other therapeutic approaches need to be further evaluated in more experimental and clinical studies.

What is the relationship between recombinant AP1G1 and autoimmune diseases?

Recombinant AP1G1 may play a role in the treatment of autoimmune diseases by regulating the activity and function of immune cells.

What are the purification methods of AP1G1 ?

The purification methods of AP1G1 include affinity chromatography, ion exchange chromatography, gel filtration chromatography and other technologies.

Are there other s with similar mechanisms of action to AP1G1 s?

At present, many s are known to have similar mechanisms of action, but their specific differences need further research and analysis.

Is it feasible to combine recombinant AP1G1 with other drugs in tumor therapy?

The recombinant AP1G1 protein can be used in combination with other drugs, but further experimental and clinical studies are needed to determine whether this is feasible.