Recombinant Human AP2M1, T7-tagged

• Cat.No.: AP2M1-70H
• Product Overview: Recombinant Human adaptor-related protein complex 2, mu 1 subunit produced inE.coliis a non-glycosylated, polypeptide chain containing amino acids (1-435) and having a total molecular mass of 50 kDa. rhAP2M1 contains T7 tag at N-terminus and is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: AP2M1-70H
• Description: AP2M1 is a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. This protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. It may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein.
• Source: Escherichia coli.
• Physical Appearance: Sterile Filtered clear solution.
• Purity: Greater than 95.0% as determined by the analysis by reducing and non-reducing SDS-PAGE coomassie blue staining.
• Formulation: Recombinant Human AP2M1 at a concentration of 0.1mg/ml containing 10 mM Tris, pH 8.0, 0.1% Triton X-100 and 0.002% NaN3.
• Applications: • ELISA • MS• Western Blotting.
• Storage: RhAP2M1 although stable at 14°Cfor 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: AP2M1 adaptor-related protein complex 2, mu 1 subunit [ Homo sapiens ]
• Synonyms: AP2M1; adaptor-related protein complex 2, mu 1 subunit; mu2; AP50; CLAPM1; clathrin-associated/assembly/adaptor protein, medium 1; plasma membrane adaptor AP-2 50kDA protein; clathrin coat adaptor protein AP50; clathrin adaptor complex AP2, mu subunit; HA2 50 kDA subunit; clathrin assembly protein complex 2 medium chain; AP-2 mu 2 chain; KIAA0109; AP-2 mu-2 chain; Clathrin coat assembly protein AP50; Clathrin coat-associated protein AP50
• Gene ID: 1173
• mRNA Refseq: NM_001025205
• Protein Refseq: NP_001020376
• MIM: 601024
• UniProt ID: Q96CW1
• Chromosome Location: 3q28
• Pathway: Endocytosis; Huntington"s disease; Gap junction trafficking and regulation; HIV Infection; Synaptic Transmission
• Function: lipid binding; protein binding; transporter activity

Reviews

02/24/2022

The reproducibility of AP2M1 is very good, and the results remain consistent even after repeated experiments, which makes me more confident in the experimental results.

04/05/2021

The short half-life and high clearance characteristics of AP2M1 enable it to reach the therapeutic concentration quickly and maintain a stable blood concentration, thus improving the therapeutic effect.

12/14/2019

In the case of adding different chemicals or drugs, the stability of this protein is still very good, indicating that it has excellent anti-interference ability and stability.

11/24/2019

AP2M1 in Western blot is very good, even if it is stored for a long time, it will not affect its performance.

Q&As

What are the applications of AP2M1 in scientific research?

AP2M1 can be used in scientific research to study the regulation mechanism of intracellular protein and the pathogenesis of disease.

What are the mechanisms of recombinant AP2M1 in cancer therapy?

AP2M1 can interfere with tumor cell signaling pathways, thereby inhibiting tumor growth and spread.

What are the remaining challenges in the research of AP2M1 ?

AP2M1 research still needs to address safety, preparation methods and dosage challenges.

Can AP2M1 be used in personalized medicine?

The personalized medicine is the development trend in the future, and we can expect the application prospect of AP2M1 in this field.

Does AP2M1 have side effects?

The side effects of recombinant AP2M1 are not known and further studies are needed to evaluate its safety.

Can AP2M1 be applied through gene therapy?

It is theoretically possible to apply recombinant AP2M1 with gene therapy, but more research is needed to confirm this.

What animal model can be used for the study of AP2M1 ?

Mouse models are currently available to further understand the pharmacological properties of AP2M1 .

Can AP2M1 be administered orally?

There are no results of studies on oral administration, but attempts to develop the pathway could be made.

What are the advantages of AP2M1 compared with drug therapy?

AP2M1 can play a therapeutic role by interfering with intracellular signaling pathways, sometimes avoiding adverse drug reactions.

What is the dosage and use of AP2M1 ?

The current dosage and mode of use are still under investigation, and further clinical trials are needed to determine the best use.

What is the future development direction of AP2M1 research?

Future studies could focus on optimizing the preparation method, study dose and safety, and further evaluate the clinical prospects of AP2M1 .

How does AP2M1 act on human cells?

AP2M1 can bind to specific receptors in human cells to regulate signaling pathways.

Can recombinant AP2M1 be used for drug delivery?

AP2M1 may be used as part of the drug delivery system to achieve targeted drug delivery by regulating intracellular delivery mechanisms.

Does AP2M1 interact with other proteins?

Yes, the recombinant AP2M1 protein can interact with other proteins to affect cell function.

How is the research progress of AP2M1 ?

AP2M1 research is still in its infancy, but there have been some promising discoveries.

What is the preparation method of AP2M1 ?

AP2M1 is generally expressed and purified in cells by genetic engineering technology.

What is the stability of AP2M1 ?

The stability of AP2M1 may be affected by a variety of factors and needs to be optimized through further research.

Does AP2M1 have antiviral activity?

AP2M1 may have antiviral activity, but further validation and research is needed.

Can recombinant AP2M1 be used to treat clinical diseases?

It is not currently in clinical use, but one can expect its potential in treating diseases such as cancer.

What are the advantages of AP2M1 in treatment?

AP2M1 has high specificity and selectivity, and can be targeted for treatment.