Recombinant Human AP4B1 293 Cell Lysate

• Cat.No.: AP4B1-8806HCL

Specification

• Description: Antigen standard for adaptor-related protein complex 4, beta 1 subunit (AP4B1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: AP4B1 adaptor-related protein complex 4, beta 1 subunit [ Homo sapiens ]
• Official Symbol: AP4B1
• Synonyms: AP4B1; adaptor-related protein complex 4, beta 1 subunit; spastic paraplegia 47 , SPG47; AP-4 complex subunit beta-1; beta 4 subunit of AP 4; BETA 4; beta4-adaptin; spastic paraplegia 47; beta 4 subunit of AP-4; CPSQ5; SPG47; BETA-4
• Gene ID: 10717
• mRNA Refseq: NM_001253852
• Protein Refseq: NP_001240781
• MIM: 607245
• UniProt ID: Q9Y6B7
• Chromosome Location: 1p13.2
• Pathway: Clathrin derived vesicle budding, organism-specific biosystem; Golgi Associated Vesicle Biogenesis, organism-specific biosystem; Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Membrane Trafficking, organism-specific biosystem; trans-Golgi Network Vesicle Budding, organism-specific biosystem
• Function: binding; protein transporter activity; transporter activity

Reviews

09/11/2021

Under different environmental conditions, the stability of AP4B1 is still very good, indicating that it has a wide range of applicability and stability.

11/21/2020

AP4B1 showed good solubility in Western blot and can be easily dissolved in various buffers.

08/30/2019

Short half-life and high clearance of AP4B1 make its effect more rapid and short, and can quickly achieve the therapeutic effect.

05/31/2019

Experimenting with this AP4B1 allows me to more easily perform experimental operations without having to worry about reproducibility of experimental results.

Q&As

How does AP4B1 participate in cellular processes?

AP4B1 is involved in intracellular protein transport and transport, and plays an important role in maintaining cell function.

What is the research progress on AP4B1?

Studies targeting the AP4B1 are actively underway to explore its function, mechanism of action, and potential therapeutic applications.

Are there any existing research projects related to AP4B1 ?

A number of related research projects have been carried out targeting the AP4B1 to understand its role in disease and potential therapeutic strategies.

May AP4B1 have side effects?

Because there are currently no approved drugs, the side effects of AP4B1 are not fully understood.

Is there any crystal structure analysis of AP4B1 ?

At present, there are no reports on the crystal structure analysis of AP4B1 , which needs future research efforts.

How does AP4B1 interact with other proteins?

The AP4B1 can interact with other proteins to form complexes that regulate cellular function.

Are there any drugs that target recombinant AP4B1 that have been approved?

There are currently no approved drugs targeting the AP4B1, but research is ongoing.

Are there any clinical trial results on recombinant AP4B1?

At present, there are no published clinical trial results on AP4B1 , which is the focus of future research.

Can AP4B1 be used by oral administration?

As no related drugs have been approved yet, the feasibility of oral administration of AP4B1 needs to be further investigated.

Can the expression of AP4B1 be regulated by gene editing technology?

Gene editing technology can be used to regulate the expression of AP4B1 , but further studies are needed to verify its feasibility and effect.

Which diseases are associated with recombinant AP4B1?

The recombinant AP4B1 protein has been implicated in several neurological diseases, such as neurodegenerative diseases and epilepsy.

Has AP4B1 been used in clinical cases?

At present, there are no reports on the application of AP4B1 in clinical cases, which is an area to be studied and developed.

What are the structural properties of AP4B1 ?

The AP4B1 has a specific three-dimensional structure, including helix and folding, which plays an important role in its function.

How soon can drugs targeting recombinant AP4B1 be available?

Drugs targeting the AP4B1 are expected to take a long time to become available, as related research and clinical trials are still ongoing.

What is the relationship between AP4B1 and immune system?

No clear studies have been reported on the relationship between AP4B1 and the immune system, which is an area of further study and we can look forward to future discoveries.

What is the clinical application value of AP4B1 ?

The recombinant AP4B1 protein has the potential to be used as a novel drug target in the clinical treatment of its related diseases.

What is the role of AP4B1 in neurological diseases?

Recombinant protein AP4B1 plays an important role in regulating nervous system function and cell signaling, and may be related to the occurrence and development of nervous system diseases.

Have specific antibodies against AP4B1 been developed?

At present, there are no reports on the development of specific antibodies against AP4B1 , which is the future research direction.

What is the synthesis method of AP4B1 ?

Recombinant AP4B1 proteins are usually synthesized using genetic engineering techniques to produce s by expressing the AP4B1 gene in cells.