Recombinant Human APBA2 protein, His & T7-tagged

• Cat.No.: APBA2-8171H
• Product Overview: Recombinant Human APBA2 aa. (Tyr459~Asn722 (Accession # Q99767)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is a member of the X11 protein family. It is a neuronal adapter protein that interacts with the Alzheimer's disease amyloid precursor protein (APP). It stabilizes APP and inhibits production of proteolytic APP fragments including the A beta peptide that is deposited in the brains of Alzheimer's disease patients. This gene product is believed to be involved in signal transduction processes. It is also regarded as a putative vesicular trafficking protein in the brain that can form a complex with the potential to couple synaptic vesicle exocytosis to neuronal cell adhesion.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 32.5kDa
• Protein length: Tyr459~Asn722 (Accession # Q99767)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 7.7.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: APBA2 amyloid beta precursor protein binding family A member 2 [ Homo sapiens (human) ]
• Official Symbol: APBA2
• Synonyms: X11L; MINT2; LIN-10; HsT16821; X11-BETA; D15S1518E; MGC:14091; X11-like protein; adapter protein X11beta; amyloid beta (A4); precursor protein-binding, family A, member 2 (X11-like); mint-2; neuron-specific X11L protein; neuronal munc18-1-interacting protein 2; phosphotyrosine-binding/-interacting domain (PTB)-bearing protein; amyloid beta A4 precursor protein-binding family A member 2
• Gene ID: 321
• mRNA Refseq: NM_001130414.1
• Protein Refseq: NP_001123886.1
• UniProt ID: Q99767

Reviews

12/13/2022

APBA2 is really amazing, and the results are very consistent every time, you can see that the production process is very careful.

07/07/2021

APBA2 they produced has good solubility and can dissolve easily.

12/14/2020

After using this recombinant protein, the quantitative accuracy of Western blot was significantly improved.

11/18/2019

The catalytic activity of APBA2 is excellent, and it can make tedious chemical reactions simple, and its simplification is to be appreciated.

Q&As

In what diseases may recombinant APBA2 have potential medical applications?

Current studies have found that the APBA2 may have a promising application in neurosystem-related diseases such as Alzheimer's disease, Parkinson's disease, and epilepsy.

What are the applications of APBA2 in drug screening?

Recombinant APBA2 protein can be used in drug screening to evaluate the activity and effectiveness of various drug candidates by detecting the binding ability and affinity of different compounds to it.

What is the research significance of APBA2 ?

As a protein regulating neuronal signaling, APBA2 has potential application prospects in the prevention and treatment of nervous system-related diseases, and is also of great significance for the study of the development, function and pathological mechanism of the nervous system.

How to evaluate the efficacy and safety of APBA2 ?

The efficacy and safety of APBA2 can be evaluated through in vitro and in vivo pharmacological experiments, toxicity tests, clinical studies and other methods.

What is the relationship between APBA2 and synaptic plasticity?

The APBA2 can promote the connection between neurons and the formation and stability of synapses, and increase the plasticity of synapses.

What is the antigenicity of APBA2 ?

At present, no relevant studies have reported the specific antigenicity of APBA2 .

What is the application prospect of APBA2 in neuroethology?

Recombinant APBA2 protein can be used as an indicator in neurobehavioral experiments to evaluate learning and memory ability in mice and rats.

How to realize the protective effect of APBA2 on neurons?

The APBA2 can promote the formation and stability of neuronal synapses and strengthen the connections between neurons, thereby protecting neurons from damage.

How to prepare APBA2 efficiently?

APBA2 protein can be expressed in cells by molecular cloning technology, and the can be purified.

What are the application prospects of recombinant APBA2 in Alzheimer's disease?

APBA2 plays an important role in regulating neuronal signaling and synaptic function, so it has potential application prospects in neurosystem-related diseases such as Alzheimer's disease.

How can APBA2 be further optimized and improved?

The performance of APBA2 protein in clinical and scientific applications can be further optimized and improved by changing its structure, enzymolysis site and affinity.

What is the molecular mechanism of APBA2 ?

APBA2 is a key protein regulating nerve cell signal transmission, which can bind to neurotransmitter receptors, promote neurotransmitter signal transmission, increase synaptic plasticity and promote neuronal differentiation and maturation.

What is the structure of APBA2 ?

The APBA2 is a protein containing multiple domains, and the three-dimensional structure of some of its structures has been analyzed, including the domain where it binds to the transmitter receptor.

In which experiments is APBA2 widely used?

Recombinant APBA2 protein has been widely used in experiments of nervous system development, neurodegenerative diseases, synaptic plasticity, drug screening and so on.

How to quantify the binding capacity of APBA2 ?

Binding quantitative detection of APBA2 can be performed based on in vitro hybridization, molecular crosslinking, biosensor and other technologies.

How does APBA2 bind to neurotransmitter receptors?

The recombinant APBA2 protein can bind to the intrinsic domain of neurotransmitter receptors, regulating neurotransmitter signaling and synaptic plasticity.

Can APBA2 be obtained through bioengineering?

Recombinant APBA2 protein can be obtained by molecular cloning technology and cell engineering technology.