Recombinant Human APOB, His-tagged
Cat.No. : | APOB-27149TH |
Product Overview : | Recombinant fragment, corresponding to amino acids 1406-1606 of Human Apolipoprotein B, fused to a His tag at N-terminal end, 31kDa. |
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Description : | This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. |
Conjugation : | HIS |
Source : | E. coli |
Form : | Liquid |
Storage buffer : | Preservative: NoneConstituents: PBS |
Storage : | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
Sequence Similarities : | Contains 1 vitellogenin domain. |
Gene Name : | APOB apolipoprotein B (including Ag(x) antigen) [ Homo sapiens ] |
Official Symbol : | APOB |
Synonyms : | APOB; apolipoprotein B (including Ag(x) antigen); apolipoprotein B-100; |
Gene ID : | 338 |
mRNA Refseq : | NM_000384 |
Protein Refseq : | NP_000375 |
Uniprot ID : | P04114 |
Chromosome Location : | 2p24-p23 |
Pathway : | Cell surface interactions at the vascular wall, organism-specific biosystem; Chylomicron-mediated lipid transport, organism-specific biosystem; FOXA1 transcription factor network, organism-specific biosystem; Fat digestion and absorption, organism-specific biosystem; Fat digestion and absorption, conserved biosystem; |
Function : | cholesterol transporter activity; enzyme binding; heparin binding; lipid transporter activity; low-density lipoprotein particle receptor binding; |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (18)
Ask a questionYes, certain disorders can lead to overproduction or excessive presence of the APOB protein. Familial dysbetalipoproteinemia is one such disorder where APOB-100 clearance by the liver is impaired, resulting in high levels of chylomicron remnants and very-low-density lipoprotein remnants in the blood. This condition is characterized by increased cardiovascular disease risk.
Yes, measuring APOB protein levels can serve as a useful diagnostic marker for cardiovascular diseases. Elevated levels of APOB-100 and LDL cholesterol are associated with an increased risk of atherosclerosis, heart attacks, and strokes. Therefore, measuring APOB protein levels can help assess an individual's cardiovascular disease risk.
Genetic factors, such as certain mutations or variations in the APOB gene, can directly impact APOB protein levels. Lifestyle factors like diet, physical activity, and smoking can also indirectly influence APOB protein levels through their effects on LDL cholesterol metabolism.
APOB protein levels can be measured through blood tests. The most common method involves measuring the concentration of APOB-100 in the bloodstream, as it is the predominant isoform associated with LDL particles. This measurement is often used as a marker of LDL cholesterol levels and cardiovascular disease risk.
Yes, diet and lifestyle factors can influence APOB levels. Consumption of a diet high in saturated fats and cholesterol can increase APOB levels and the production of LDL cholesterol. Conversely, a diet rich in fruits, vegetables, whole grains, and healthy fats (such as monounsaturated fats and omega-3 fatty acids) can help lower APOB levels and improve lipid profile.
Yes, aside from its well-established involvement in lipid metabolism and cardiovascular diseases, ongoing research is investigating the role of the APOB protein in other conditions such as non-alcoholic fatty liver disease (NAFLD), metabolic syndrome, and certain types of cancer. This research aims to further understand the complex functions and potential therapeutic implications of the APOB protein.
Yes, the APOB-48 isoform of the APOB protein plays a key role in lipid absorption from the intestines. Chylomicrons, which are lipoprotein particles responsible for transporting dietary fats, contain APOB-48. It facilitates the incorporation of triglycerides into chylomicrons, allowing them to transport dietary fats to the bloodstream.
Yes, genetic mutations in the APOB gene can lead to alterations in the APOB protein's structure or function. For instance, certain mutations can result in familial hypercholesterolemia, a condition characterized by elevated LDL cholesterol levels and increased risk of cardiovascular disease. These mutations can interfere with the binding of LDL particles to receptors on liver cells, impairing their clearance from the bloodstream.
No, currently, there are no medications specifically designed to target the APOB protein itself. However, various lipid-lowering medications, such as statins, work indirectly by reducing the production of APOB-100 or suppressing LDL cholesterol synthesis. These medications ultimately reduce the APOB protein levels and improve lipid management.
Yes, there is a correlation between APOB levels and body weight or obesity. Studies have shown that obese individuals often have higher levels of APOB-100 and LDL cholesterol. The excess adipose tissue in obesity can lead to dysregulation in lipid metabolism and contribute to increased APOB production and LDL cholesterol levels.
Dysfunction or abnormalities in APOB protein function can have significant implications for lipid metabolism and cardiovascular health. Decreased APOB protein levels or impaired APOB-mediated lipid transportation can lead to elevated LDL cholesterol levels, which are a known risk factor for atherosclerosis and heart disease.
While the primary role of the APOB protein is in lipid transportation, some studies suggest that it may have implications in brain function and neurodegenerative diseases. APOB-containing lipoproteins have been found in the brain, and there is emerging evidence linking APOB to Alzheimer's disease pathology. However, further research is needed to understand the specific mechanisms and potential therapeutic implications.
Yes, there are therapeutic interventions aimed at targeting the APOB protein and reducing LDL cholesterol levels. Medications called statins, which inhibit cholesterol synthesis, are commonly prescribed to lower LDL cholesterol. Additionally, other lipid-lowering drugs, such as PCSK9 inhibitors and bile acid sequestrants, can also indirectly affect APOB protein levels.
The APOB protein is produced in the liver and intestines through a process called translation. The APOB gene undergoes a unique form of RNA editing known as apolipoprotein B mRNA editing catalytic polypeptide-like (APOBEC) editing, which leads to the production of two protein isoforms: APOB-100, found in LDL particles, and APOB-48, found in chylomicrons.
Yes, dietary interventions can impact APOB protein levels indirectly by influencing LDL cholesterol levels. Consuming a diet low in saturated fats, trans fats, and cholesterol, while high in fiber and healthy fats like omega-3 fatty acids, can help reduce LDL cholesterol levels and potentially lower APOB protein concentration.
APOB levels can vary throughout the day, although they generally remain relatively stable. However, several factors, such as meal consumption, can temporarily increase APOB levels due to the production and release of chylomicrons from the intestines. Regular fasting lipid tests are typically done after an overnight fast to minimize the impact of these temporary fluctuations.
Yes, APOB levels can be used to monitor the effectiveness of lipid-lowering therapies. Since APOB is a major component of LDL cholesterol and other atherogenic lipoproteins, measuring APOB levels can provide valuable information about the impact of lipid-lowering medications or lifestyle modifications on reducing atherogenic lipoproteins and cardiovascular risk. Regular monitoring of APOB levels can help assess the effectiveness of lipid-lowering therapies and guide treatment decisions.
Yes, genetic variations and mutations in the APOB gene can occur. Some rare genetic variations in the APOB gene can lead to familial hypercholesterolemia, a condition characterized by abnormally high levels of LDL cholesterol. These mutations disrupt normal APOB protein function, resulting in impaired lipid transportation and increased cardiovascular disease risk.
Customer Reviews (4)
Write a reviewIn protein electron microscopy structure analysis, APOB protein proved to be an invaluable tool, allowing for the precise determination of protein structures with remarkable clarity and resolution.
In my ELISA experiments, APOB protein consistently provided reliable and reproducible results, with high sensitivity and specificity.
I enthusiastically endorse APOB protein as it delivers exceptional performance in a wide range of applications.
This unequivocal detection and visualization of protein bands were instrumental in facilitating accurate analysis and interpretation of my experimental data.
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