Recombinant Human APOBEC3G protein

• Cat.No.: APOBEC3G-1576H
• Product Overview: Recombinant APOBEC3G was expressed in and purified from E. coli.

Specification

• Description: Homo sapiens apolipoprotein B mRNA editing enzyme, catalytic polypeptidelike 3G. This protein is a member of the cytidine deaminase family. They are
  structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that APOBEC proteins may be RNA editing
  enzymes and have roles in growth or cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been identified.
• Source: E. coli
• Species: Human
• Form: 20 mM Tris-HCl, pH 7.5, 300 mM NaCl, and 10% glycerol.
• Molecular Mass: 46 kDa

Gene Information

• Gene Name: APOBEC3G apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G [ Homo sapiens ]
• Official Symbol: APOBEC3G
• Synonyms: APOBEC3G; apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G; DNA dC->dU-editing enzyme APOBEC-3G; bK150C2.7; CEM15; dJ494G10.1; FLJ12740; MDS019; DNA dC-> dU-editing enzyme APOBEC-3G; APOBEC-related protein 9; dU editing enzyme; phorbolin-like protein MDS019; APOBEC-related cytidine deaminase; apolipoprotein B mRNA editing enzyme cytidine deaminase; ARCD; ARP9; ARP-9; CEM-15
• Gene ID: 60489
• mRNA Refseq: NM_021822
• Protein Refseq: NP_068594
• MIM: 607113
• UniProt ID: Q9HC16

Reviews

03/13/2023

Manufacturers can ensure high-quality production of APOBEC3G protein, maintaining its purity and integrity.

07/22/2022

Its resistance to degradation and denaturation allows for longer storage times and repeated use without compromising its performance or functionality.

05/18/2016

This guarantees the reliability and reproducibility of research findings and eliminates potential issues arising from impurities or contamination.

04/18/2016

When used in Western blotting experiments, the APOBEC3G protein produces distinct protein bands, allowing for easy visualization and interpretation.

Q&As

Can APOBEC3G be counteracted by viruses?

Yes, certain viruses have developed mechanisms to counteract the antiviral activity of APOBEC3G. For example, HIV-1 produces the viral protein Vif, which targets APOBEC3G for degradation and prevents its incorporation into viral particles.

Are there any genetic variants of APOBEC3G that affect its antiviral activity?

Yes, certain single nucleotide polymorphisms (SNPs) in the APOBEC3G gene have been associated with alterations in its antiviral activity. These genetic variants can influence the efficacy of APOBEC3G-mediated defense against viral infections.

Is APOBEC3G specific to a particular type of virus?

APOBEC3G exhibits broad antiviral activity and can potentially inhibit a variety of retroviruses. However, its effectiveness against different viruses may vary due to viral countermeasures and the interplay of viral and cellular factors.

How does APOBEC3G affect the evolution of viruses?

APOBEC3G can exert selective pressure on viruses by inducing hypermutation. This can lead to the generation of viral sequence diversity, potentially reducing viral fitness and accelerating the evolution of viral escape mutants that are less susceptible to APOBEC3G activity.

Is APOBEC3G involved in cancer development?

APOBEC3G can be dysregulated in certain cancer types and has been implicated in cancer development and progression. Its mutagenic activity can lead to the accumulation of genetic mutations in cancer cells, promoting tumor evolution.

Are there any diseases or conditions associated with dysregulated APOBEC3G activity?

Dysregulated APOBEC3G activity has been implicated in various diseases and conditions. For example, APOBEC3G-mediated hypermutation has been linked to the development of certain cancers, such as bladder cancer and multiple myeloma. Additionally, dysregulation of APOBEC3G activity has been associated with autoimmune diseases and viral persistence.

Is APOBEC3G activity limited to viral genomes or can it also target other nucleic acids?

APOBEC3G primarily targets viral genomes during reverse transcription. However, under certain conditions, it can also deaminate endogenous and non-viral DNA or RNA species, leading to genomic instability and potential damage to host cells. The regulation of APOBEC3G activity helps restrict its effects to viral nucleic acids while minimizing off-target effects.

Has APOBEC3G been implicated in antiviral defense against other types of viruses apart from retroviruses?

While APOBEC3G is primarily known for its role in defense against retroviruses, there is emerging evidence that it may also have antiviral activity against other virus families, such as hepadnaviruses, paramyxoviruses, and DNA viruses. However, further studies are needed to fully elucidate its antiviral capabilities beyond retroviruses.

Can APOBEC3G be incorporated into viral particles?

Yes, under normal circumstances, APOBEC3G can be packaged into viral particles and transferred to newly infected cells. However, the presence of viral protein Vif in certain viruses, like HIV-1, prevents the incorporation of APOBEC3G into viral particles.

How does APOBEC3G protect against viral infections?

APOBEC3G exerts its antiviral activity by inhibiting the replication of retroviruses, including HIV-1. It functions by causing G-to-A hypermutations in the viral genome during reverse transcription, resulting in viral genome degradation and decreased infectivity.

Are there any other members in the APOBEC protein family?

Yes, besides APOBEC3G, there are several other members in the APOBEC family, including APOBEC3A, APOBEC3B, APOBEC3C, APOBEC3D, APOBEC3F, APOBEC3H, and APOBEC3L. Each member has distinct functions and tissue-specific expression patterns.

Is APOBEC3G unique to humans or present in other species?

APOBEC3G is not unique to humans and is found in various other species, including primates, mice, and cats. These APOBEC3G orthologs exhibit similar antiviral activities, indicating a conserved role in innate immunity against retroviruses.

Can APOBEC3G be targeted for cancer treatment?

Targeting APOBEC3G for cancer treatment is an area of ongoing research. Strategies aim to either inhibit its mutagenic activity to reduce tumor evolution or exploit its activity to induce lethal levels of mutations selectively in cancer cells.

What are the consequences of APOBEC3G-mediated hypermutation in viral genomes?

Hypermutation induced by APOBEC3G can lead to the introduction of stop codons, frame shifts, and alterations in important viral protein coding regions. This can impair viral replication, decrease infectivity, and increase the risk of viral extinction.

Is there any link between APOBEC3G and autoimmune diseases?

Studies have shown that APOBEC3G activity can contribute to autoimmune diseases. The aberrant expression and function of APOBEC3G may lead to the generation of self-reactive antibodies and contribute to the development of autoimmune responses.

Can APOBEC3G be therapeutically targeted for HIV treatment?

Targeting APOBEC3G for HIV treatment is a promising approach. Strategies under investigation include inhibiting viral protein Vif, which degrades APOBEC3G, or developing small molecules that enhance APOBEC3G's antiviral potency to limit viral replication.

Are there any interactions or partnerships between APOBEC3G and other proteins?

APOBEC3G can interact with various proteins to influence its antiviral activity. For example, it can form complexes with other APOBEC proteins, such as APOBEC3F and APOBEC3H, to enhance their antiviral potency. Furthermore, APOBEC3G can interact with host proteins involved in RNA editing and DNA repair pathways.

Does APOBEC3G have any non-antiviral functions?

Although primarily known for its antiviral activity, recent research suggests that APOBEC3G may have non-antiviral functions. It has been implicated in DNA demethylation processes and can potentially influence cellular genomic stability and gene regulation.

Can APOBEC3G be used as a therapeutic target for cancer treatment?

APOBEC3G is being investigated as a potential therapeutic target for cancer treatment. Inhibiting APOBEC3G activity in cancer cells with dysregulated expression can help reduce mutational burden and genomic instability, potentially slowing down tumor progression and improving treatment outcomes.

Can APOBEC3G activity lead to off-target mutations in the host genome?

APOBEC3G has the potential to generate mutations in the host genome. However, there are mechanisms in place to restrict its activity to viral nucleic acids and prevent excessive off-target mutations in the host genome.

Are there any therapeutic strategies targeting APOBEC3G?

Several therapeutic strategies have been proposed to target APOBEC3G. These include developing small molecules or peptides that can enhance APOBEC3G's antiviral activity, or gene therapy approaches to increase its expression in target cells.

Is APOBEC3G activity regulated by cellular factors?

Yes, APOBEC3G activity can be regulated by cellular factors. Factors like RNA binding proteins, post-translational modifications, and protein-protein interactions can modulate its stability, localization, and antiviral function.

Can APOBEC3G be used as a biomarker for disease progression or prognosis?

The potential use of APOBEC3G as a biomarker is currently under investigation. Studies have suggested that APOBEC3G expression levels or genetic variants may be associated with disease prognosis, treatment response, or disease progression in certain cancers and viral infections. However, further research is needed to establish its clinical utility.