Recombinant Human AQP10 293 Cell Lysate

• Cat.No.: AQP10-8769HCL

Specification

• Description: Antigen standard for aquaporin 10 (AQP10) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: AQP10 aquaporin 10 [ Homo sapiens ]
• Official Symbol: AQP10
• Synonyms: AQP10; aquaporin 10; aquaporin-10; AQP-10; aquaglyceroporin-10; small intestine aquaporin; AQPA_HUMAN
• Gene ID: 89872
• mRNA Refseq: NM_080429
• Protein Refseq: NP_536354
• MIM: 606578
• UniProt ID: Q96PS8
• Chromosome Location: 1q21.3
• Pathway: Aquaporin-mediated transport, organism-specific biosystem; Passive Transport by Aquaporins, organism-specific biosystem; Transmembrane transport of small molecules, organism-specific biosystem
• Function: transporter activity

Reviews

10/11/2022

The exceptional performance of AQP10 protein in both ELISA and protein electron microscopy structure analysis positions it as a valuable tool in a broad range of research applications.

04/19/2021

Researchers have successfully utilized AQP10 protein in electron microscopy studies, unraveling crucial insights into protein interactions and conformational changes.

02/22/2021

This can involve organizing conferences, workshops, and webinars to discuss the latest advancements, share research findings, and broaden the understanding of AQP10 function.

11/02/2017

AQP10 protein demonstrates outstanding performance in ELISA assays, making it highly recommended for researchers and scientists.

Q&As

Can AQP10 be targeted for drug development?

The potential of targeting AQP10 for drug development is still being explored. Modulating AQP10 activity or expression may have therapeutic implications in disorders involving intestinal water and glycerol transport abnormalities. However, more research is needed to understand the feasibility and safety of targeting AQP10 for drug development.

Are there any known interactions or modulators of AQP10 function?

Currently, there is limited information on specific molecules that interact with AQP10 or modulate its function. Further research is required to uncover potential regulators or interaction partners of AQP10.

Is there any clinical relevance or potential diagnostic applications of AQP10?

Currently, there are no established clinical applications of AQP10 in diagnostics. However, future research may uncover diagnostic markers or therapeutic targets related to AQP10 in specific intestinal disorders or conditions.

Can AQP10 be used as a potential diagnostic marker for certain diseases?

The diagnostic potential of AQP10 in specific diseases is still being explored. Some studies have investigated its expression in different tissues and its association with various conditions. However, more research is needed to determine if AQP10 could serve as a reliable diagnostic marker.

Can targeting AQP10 have therapeutic implications?

Given the importance of AQP10 in water and glycerol transport in the intestine, targeting this protein may have therapeutic implications in conditions associated with altered fluid balance in the intestine. However, further studies are needed to evaluate the therapeutic potential and safety of targeting AQP10 in a clinical setting.

How does AQP10 differ from other aquaporin proteins?

Aquaporin proteins share a high degree of sequence similarity, but they have distinct tissue distribution and functional properties. AQP10 differs from other aquaporins in its expression pattern, being primarily found in the intestine, and its ability to transport both water and glycerol.

Does AQP10 have any other physiological functions outside of the intestine?

Although AQP10 is primarily expressed in the intestine, some studies suggest that it may also play a role in other tissues, such as the liver and kidney. Further research is required to fully understand the physiological functions of AQP10 in different organs.

Is AQP10 polymorphism associated with any physiological traits or diseases?

Some studies suggest that AQP10 polymorphisms may be associated with altered water and glycerol transport in the intestines, but the significance of these polymorphisms in relation to specific physiological traits or diseases requires further investigation.

Are there any known genetic mutations in the AQP10 gene?

There is limited information on genetic mutations in the AQP10 gene. However, some studies have identified single nucleotide polymorphisms (SNPs) in the AQP10 gene that may be associated with altered intestinal water and glycerol transport.

Can AQP10 be targeted for therapeutic interventions?

The potential therapeutic targeting of AQP10 is being investigated for various conditions. For example, modulating AQP10 expression or activity may have applications in managing intestinal disorders such as IBD. However, the development of specific AQP10-targeted therapies is still in its early stages and requires further study.

Are there any specific diseases or conditions associated with mutations in the AQP10 gene?

Currently, there is limited evidence linking specific diseases or conditions directly to mutations in the AQP10 gene. However, some studies suggest that AQP10 dysfunction may be associated with intestinal disorders such as inflammatory bowel disease (IBD) and obesity.

What are the potential implications of AQP10 research for drug delivery or water management technologies?

AQP10 research may have implications for drug delivery systems and water management technologies. Understanding the mechanisms and regulation of AQP10 could aid in the development of targeted drug delivery approaches and improved water filtration or desalination techniques. However, this application is mainly speculative at this stage and requires further investigation.

Are there any known diseases or conditions associated with AQP10 dysfunction?

Currently, there is limited research on specific diseases or conditions directly linked to AQP10 dysfunction. However, it is believed that alterations in AQP10 expression or activity may contribute to intestinal disorders related to water and glycerol transport dysregulation.

Does AQP10 have any implications in cancer research?

A few studies have suggested possible roles of AQP10 in cancer progression. It has been found to be upregulated in certain cancer types, including colorectal and pancreatic cancers. However, further research is necessary to fully understand the implications of AQP10 in cancer development and progression.

Are there any animal models that mimic AQP10-related conditions for research purposes?

Currently, there are no known animal models specifically designed to mimic AQP10-related conditions. However, researchers may use knockdown or knockout models to study the effects of AQP10 manipulation on intestinal physiology in animals.

Are there any ongoing clinical trials related to AQP10?

Currently, there are no registered clinical trials specifically focused on AQP10. However, it is possible that research related to AQP10 may be included as part of broader studies on intestinal water and glycerol transport or related conditions.

Are there any known inhibitors or activators of AQP10?

As of now, there are no known specific inhibitors or activators of AQP10. Developing targeted modulators of AQP10 activity is an area of ongoing research, but significant progress in identifying such compounds has not been reported.

Is AQP10 primarily involved in water transport or glycerol transport?

AQP10 is thought to play a role in both water and glycerol transport. It acts as a channel for the movement of water molecules across the cell membrane and also facilitates the transport of glycerol, a small hydrophilic molecule.