Recombinant Human AQP3 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: AQP3-6031H
• Product Overview: AQP3 MS Standard C13 and N15-labeled recombinant protein (NP_004916) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 31.5 kDa
• AA Sequence: MGRQKELVSRCGEMLHIRYRLLRQA LAECLGTLILVMFGCGSVAQVVLSR GTHGGFLTINLAFGFAVTLGILIAG QVSGAHLNPAVTFAMCFLAREPWIK LPIYTLAQTLGAFLGAGIVFGLYYD AIWHFADNQLFVSGPNGTAGIFATY PSGHLDMINGFFDQFIGTASLIVCV LAIVDPYNNPVPRGLEAFTVGLVVL VIGTSMGFNSGYAVNPARDFGPRLF TALAGWGSAVFTTGQHWWWVPIVSP LLGSIAGVFVYQLMIGCHLEQPPPS NEEENVKLAHVKHKEQITRTRPLEQ KLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: AQP3 aquaporin 3 (Gill blood group) [ Homo sapiens (human) ]
• Official Symbol: AQP3
• Synonyms: AQP3; aquaporin 3 (Gill blood group); aquaporin 3, aquaporin 3 (GIL blood group); aquaporin-3; GIL; Gill blood group; aquaglyceroporin-3; aquaporin 3 (GIL blood group); AQP-3
• Gene ID: 360
• mRNA Refseq: NM_004925
• Protein Refseq: NP_004916
• MIM: 600170
• UniProt ID: Q92482

Reviews

02/02/2022

This level of support saves researchers time and resources, enabling them to focus on the scientific aspects of their work and accelerate the overall progress of their studies.

08/27/2021

its high quality and purity ensure accurate and reliable results.

02/02/2019

If researchers require specific variants or modifications of AR protein, the manufacturer can work with them to provide customized products tailored to their needs.

06/30/2018

Manufacturers may offer the option for custom protein production.

03/06/2018

The AQP3 protein exhibits several valuable attributes that make it an excellent choice for my trials.

04/17/2017

With the manufacturer's unwavering dedication to delivering quality products and unparalleled technical support, I am confident in their ability to help me overcome any challenges and propel my research forward.

04/17/2017

the stability of the AQP3 protein enables me to conduct experiments over an extended period, reducing the need for frequent restocking and promoting experimental consistency.

01/10/2016

Their expertise can help troubleshoot problems, suggest alternative approaches, or provide recommendations for optimizing experimental protocols.

Q&As

Are there any known disorders or conditions associated with AQP3 dysfunction?

Yes, mutations or alterations in the AQP3 gene have been associated with several skin disorders, including palmoplantar keratoderma and porokeratosis. These conditions are characterized by abnormal skin thickening and the formation of scaly patches.

Is genetic testing available for AQP3-related disorders?

Yes, genetic testing can be conducted to identify mutations in the AQP3 gene that may be associated with specific skin disorders. This testing can help with diagnosis and provide valuable information for understanding the underlying cause of the condition.

Can lifestyle factors or environmental factors affect AQP3 activity?

Yes, certain lifestyle factors and environmental conditions can influence AQP3 activity. For example, exposure to ultraviolet radiation from sunlight can upregulate AQP3 expression in the skin, potentially affecting skin hydration and barrier function. Additionally, some studies suggest that certain drugs, such as retinoids, may modulate AQP3 expression and function.

Can AQP3 dysfunction lead to any other health issues?

AQP3 dysfunction may be involved in the development of some metabolic disorders and certain types of cancer. Dysregulation of AQP3 expression or activity has been linked to altered water transport and cell proliferation, which can contribute to disease progression.

Does AQP3 play a role in the regulation of body temperature?

While AQP3 primarily functions as a water transporter, there is evidence suggesting its involvement in thermoregulation. Studies have shown that AQP3 expression increases in response to heat exposure, and it may facilitate the movement of water and thermal energy across the skin, contributing to cooling mechanisms in the body.

Does AQP3 play a role in the regulation of body temperature?

While AQP3 primarily functions as a water transporter, there is evidence suggesting its involvement in thermoregulation. Studies have shown that AQP3 expression increases in response to heat exposure, and it may facilitate the movement of water and thermal energy across the skin, contributing to cooling mechanisms in the body.

Can AQP3 be targeted for therapeutic purposes?

AQP3 has been investigated as a potential therapeutic target in various contexts. For example, in dermatology, compounds that modulate AQP3 activity are being explored for their ability to improve skin hydration and barrier function. In cancer research, inhibiting AQP3 expression or activity has shown potential in impeding tumor growth and metastasis.

Can AQP3 be targeted for therapeutic purposes?

AQP3 has been investigated as a potential therapeutic target in various contexts. For example, in dermatology, compounds that modulate AQP3 activity are being explored for their ability to improve skin hydration and barrier function. In cancer research, inhibiting AQP3 expression or activity has shown potential in impeding tumor growth and metastasis.

Can AQP3 dysfunction affect the hydration of the skin?

Yes, alterations in AQP3 function or expression can impact skin hydration. Decreased AQP3 activity has been associated with impaired water transport across skin cells, leading to decreased skin hydration and potentially contributing to dry skin conditions.

Can AQP3 dysfunction affect the hydration of the skin?

Yes, alterations in AQP3 function or expression can impact skin hydration. Decreased AQP3 activity has been associated with impaired water transport across skin cells, leading to decreased skin hydration and potentially contributing to dry skin conditions.

Are there any treatments available for AQP3-related disorders?

Currently, there are no specific targeted treatments available for AQP3-related disorders. However, management of symptoms and complications associated with these conditions may involve moisturizers, topical medications, and other dermatological interventions to help alleviate skin-related issues.

Is AQP3 expressed only in humans?

No, AQP3 is not exclusive to humans. It is found in various animal species, including mammals, birds, and reptiles. Its presence in different organisms suggests its evolutionary significance in water balance regulation and other biological processes.

How is the AQP3 protein regulated in the body?

The expression and activity of AQP3 can be regulated by various factors. Hormones, such as glucocorticoids and thyroid hormones, have been shown to influence AQP3 expression in certain tissues. Additionally, signaling pathways like the cAMP-protein kinase A pathway and the MAPK pathway have been implicated in the regulation of AQP3 activity.

How is the AQP3 protein regulated in the body?

The expression and activity of AQP3 can be regulated by various factors. Hormones, such as glucocorticoids and thyroid hormones, have been shown to influence AQP3 expression in certain tissues. Additionally, signaling pathways like the cAMP-protein kinase A pathway and the MAPK pathway have been implicated in the regulation of AQP3 activity.

Are there any known inhibitors or activators of AQP3?

Several compounds have been identified as potential AQP3 inhibitors or activators. For example, some natural substances like flavonoids and polyphenols have been found to inhibit AQP3 function. Conversely, certain synthetic compounds, such as AQP3 activators 4-Chlorobenzoylisothiocyanate and phloretin, have been shown to enhance AQP3 activity.

Are there any known inhibitors or activators of AQP3?

Several compounds have been identified as potential AQP3 inhibitors or activators. For example, some natural substances like flavonoids and polyphenols have been found to inhibit AQP3 function. Conversely, certain synthetic compounds, such as AQP3 activators 4-Chlorobenzoylisothiocyanate and phloretin, have been shown to enhance AQP3 activity.

Are there any other proteins that interact with AQP3?

Yes, AQP3 can interact with other proteins within the cell. For instance, it has been shown to interact with ezrin, a cytoskeletal protein, in the kidney. This interaction may play a role in the intracellular trafficking of AQP3 and its localization to specific membrane domains.

Are there any other proteins that interact with AQP3?

Yes, AQP3 can interact with other proteins within the cell. For instance, it has been shown to interact with ezrin, a cytoskeletal protein, in the kidney. This interaction may play a role in the intracellular trafficking of AQP3 and its localization to specific membrane domains.

Can AQP3 be targeted for drug delivery purposes?

AQP3 has been explored for drug delivery applications. Due to its water transport function, it can potentially be utilized to improve the delivery of hydrophilic drugs. By understanding the mechanisms of AQP3-mediated water transport, researchers are exploring ways to engineer drug carriers that can exploit these channels for efficient drug delivery.

Can AQP3 be targeted for drug delivery purposes?

AQP3 has been explored for drug delivery applications. Due to its water transport function, it can potentially be utilized to improve the delivery of hydrophilic drugs. By understanding the mechanisms of AQP3-mediated water transport, researchers are exploring ways to engineer drug carriers that can exploit these channels for efficient drug delivery.