Recombinant Human ARAP2, His-tagged

• Cat.No.: ARAP2-9791H
• Product Overview: Recombinant Human ARAP2 protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Description: The protein encoded by this gene contains ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology domains. The protein is a phosphatidylinositol (3,4,5)-trisphosphate-dependent Arf6 GAP that binds RhoA-GTP, but it lacks the predicted catalytic arginine in the RHO-GAP domain and does not have RHO-GAP activity. The protein associates with focal adhesions and functions downstream of RhoA to regulate focal adhesion dynamics.
• Source: E.coli
• Species: Human
• Tag: His
• Protein length: C-term-349a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.

Gene Information

• Gene Name: ARAP2 ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 [ Homo sapiens ]
• Official Symbol: ARAP2
• Synonyms: ARAP2; ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2; centaurin, delta 1 , CENTD1; arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2; PARX; cnt-d1; centaurin-delta-1; centaurin, delta 1; Arf and Rho GAP adapter protein 2; CENTD1; FLJ13675; FLJ44916; KIAA0580
• Gene ID: 116984
• mRNA Refseq: NM_015230
• Protein Refseq: NP_056045
• MIM: 606645
• UniProt ID: Q8WZ64
• Chromosome Location: 4p15.1
• Pathway: Arf6 signaling events, organism-specific biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; Rho GTPase cycle, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by Rho GTPases, organism-specific biosystem
• Function: ARF GTPase activator activity; metal ion binding; phosphatidylinositol-3,4,5-trisphosphate binding; zinc ion binding

Reviews

12/07/2021

the ARAP2 protein has proven to be an invaluable tool in protein electron microscopy (EM) structure analysis.

09/13/2021

This protein demonstrates great performance in WB assays, providing accurate and reliable results that are crucial for protein analysis.

05/15/2019

The ARAP2 protein is of exceptional quality, making it an ideal choice to meet my experimental needs.

11/09/2018

Their team of experts is readily available to address any issues that may arise, providing solutions to problems and guiding me through the experimental process.

06/13/2017

The ARAP2 protein is an outstanding product that possesses exceptional quality, making it an ideal choice for my experimental needs.

07/08/2016

What sets ARAP2 apart is the manufacturer's unparalleled technical support.

04/16/2016

The versatility of the ARAP2 protein makes it a valuable asset in my research.

01/07/2016

The ARAP2 protein is highly recommended for researchers conducting protein studies, especially in Western blotting (WB) experiments.

Q&As

How is the ARAP2 protein regulated within the cell?

The activity of the ARAP2 protein can be regulated through various mechanisms. One important regulatory process is protein phosphorylation. Phosphorylation of specific residues within ARAP2 can modulate its activity and interactions with other proteins. Additionally, ARAP2 can be regulated through protein-protein interactions, where binding to certain proteins can influence its localization, binding partners, or activity.

Are there any known diseases or conditions associated with dysregulation of ARAP2?

Yes, as mentioned earlier, specific mutations or variations in the ARAP2 gene have been linked to neurodevelopmental disorders such as autism spectrum disorder (ASD) and attention-deficit/hyperactivity disorder (ADHD). Dysregulation of ARAP2 in these disorders suggests its involvement in neuronal development, synaptic function, and potentially other processes relevant to brain function and behavior.

Are there any ongoing research studies or clinical trials investigating the ARAP2 protein?

Currently, there do not appear to be any ongoing clinical trials specifically focused on the ARAP2 protein. However, there is ongoing research exploring ARAP2's involvement in neurodevelopmental disorders, cancer biology, and other cellular processes. These studies aim to better understand its function and potential therapeutic implications.

Is ARAP2 involved in any specific cellular processes or pathways related to cancer?

ARAP2 has been implicated in several cellular processes that are relevant to cancer progression. It is involved in cell migration and invasion, both of which play crucial roles in tumor metastasis. Additionally, ARAP2 has been observed to affect cell proliferation and survival pathways, suggesting its potential involvement in cancer cell growth. However, more research is needed to fully understand the specific role of ARAP2 in cancer biology.

Are there any known inhibitors or activators of the ARAP2 protein?

Currently, there are no known specific inhibitors or activators targeting the ARAP2 protein. However, this does not rule out the possibility of future discoveries or development of compounds that modulate its activity. Further research is needed to identify and characterize ARAP2-specific modulators.

Is the ARAP2 protein involved in any signaling pathways?

Yes, the ARAP2 protein is involved in multiple signaling pathways. It interacts with different molecules and proteins, including small GTPases, receptor tyrosine kinases, and cell adhesion molecules, to regulate downstream signaling cascades. ARAP2 has been implicated in pathways such as the Rho family GTPase signaling pathway, phosphoinositide signaling pathway, and integrin-mediated signaling.

Are there any known genetic variations or mutations in the ARAP2 gene associated with diseases?

Some genetic variations or mutations in the ARAP2 gene have been associated with certain conditions. For example, specific mutations in the ARAP2 gene have been linked to neurodevelopmental disorders, including autism spectrum disorder (ASD) and attention-deficit/hyperactivity disorder (ADHD). However, more research is needed to fully understand the role of ARAP2 gene variations in these disorders.

Can the ARAP2 protein be targeted for potential therapeutic interventions?

Targeting the ARAP2 protein itself is yet to be explored for therapeutic interventions. However, as ARAP2 is involved in various signaling pathways and cellular processes, it is possible that targeting downstream effectors or pathways connected to ARAP2 could have therapeutic potential. It would require further investigation to identify potential druggable targets within these pathways.