Recombinant Human ARF5 protein, GST-tagged
Cat.No. : | ARF5-748H |
Product Overview : | Human ARF5 full-length ORF ( NP_001653.1, 1 a.a. - 180 a.a.) recombinant protein with GST-tag at N-terminal. |
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Description : | This gene is a member of the human ADP-ribosylation factor (ARF) gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. [provided by RefSeq, Dec 2010] |
Source : | Wheat Germ |
Species : | Human |
Tag : | GST |
Molecular Mass : | 46.9 kDa |
AA Sequence : | MGLTVSALFSRIFGKKQMRILMVGL DAAGKTTILYKLKLGEIVTTIPTIG FNVETVEYKNICFTVWDVGGQDKIR PLWRHYFQNTQGLIFVVDSNDRERV QESADELQKMLQEDELRDAVLLVFA NKQDMPNAMPVSELTDKLGLQHLRS RTWYVQATCATQGTGLYDGLDWLSH ELSKR |
Applications : | Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array |
Notes : | Best use within three months from the date of receipt of this protein. |
Storage : | Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing. |
Storage Buffer : | 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer. |
Gene Name : | ARF5 ADP-ribosylation factor 5 [ Homo sapiens ] |
Official Symbol : | ARF5 |
Synonyms : | ARF5; ADP-ribosylation factor 5; |
Gene ID : | 381 |
mRNA Refseq : | NM_001662 |
Protein Refseq : | NP_001653 |
MIM : | 103188 |
UniProt ID : | P84085 |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (32)
Ask a questionYes, ARF5 protein can interact with the actin cytoskeleton. It has been shown to associate with actin filaments and regulate their organization and dynamics during processes such as cell migration and neurite outgrowth.
Yes, ARF5 has been implicated in neuronal development and neurite outgrowth. It regulates membrane trafficking events required for the extension and branching of neuronal processes, contributing to proper neuronal morphology and connectivity during development.
ARF5 plays a crucial role in the formation and maturation of clathrin-coated vesicles during endocytosis. It regulates the recruitment and assembly of clathrin and adaptors to the plasma membrane, facilitating the internalization of cargo molecules.
Yes, researchers have used cell lines and genetically modified mice to study the function and regulation of ARF5. These models help in understanding the physiological significance of ARF5 in cellular processes and its potential implications in disease.
While specific disease associations with ARF5 mutations are not well-established, abnormalities in ARF5 expression or function have been implicated in certain human disorders, including cancer and neurodevelopmental disorders.
Yes, ARF5 can interact with other ARF family members, such as ARF1 and ARF6. These interactions contribute to the regulation of intracellular trafficking and signaling processes.
ARF5 is involved in various cellular processes, including endocytic pathway, vesicle trafficking, Golgi apparatus maintenance, and signaling events.
While ARF5 has been implicated in various diseases, including cancer and neurodevelopmental disorders, the specific mechanisms and associations are still being investigated. The existing literature provides a foundation for further exploration in this area.
Yes, ARF5 can affect cell signaling by regulating the trafficking and distribution of signaling receptors and molecules. It can modulate the localization and activity of certain receptors, leading to alterations in downstream signaling pathways.
Yes, ARF5 participates in vesicle trafficking between various organelles, including the Golgi apparatus and endosomes. It helps in the formation and sorting of vesicles carrying cargo between these compartments.
ARF5 is involved in the regulation of insulin secretion in pancreatic beta cells. It helps in the maintenance and organization of insulin granules within beta cells. ARF5 activity is required for the proper trafficking and fusion of insulin granules with the plasma membrane, leading to the release of insulin.
Yes, ARF5 protein is involved in various cellular processes in addition to membrane trafficking. It has been implicated in cell migration, cell proliferation, cellular signaling, and cytoskeletal organization. ARF5 can modulate these processes through its regulation of vesicle trafficking and membrane dynamics.
Yes, ARF5 plays a role in recycling membrane proteins from endosomes back to the plasma membrane. It regulates the formation of recycling vesicles and the sorting of cargo molecules in the recycling pathway, ensuring proper membrane protein localization and cell surface expression.
There are currently no known diseases specifically linked to ARF5 mutations. However, alterations in ARF5 function or expression have been observed in cancer progression, neurodevelopmental disorders, and insulin secretion defects. Further research is needed to better understand the role of ARF5 in disease processes.
ARF5 is primarily localized to the cytoplasm of cells. However, it can also associate with specific membranes and organelles, such as the Golgi apparatus and endosomes, where it plays a role in regulating vesicle trafficking.
There are currently no specific inhibitors or activators of ARF5 protein available. However, the activity of ARF5 can be regulated indirectly through its upstream regulators, such as GEFs and GAPs. Small molecules that target these regulators could potentially modulate ARF5 activity.
ARF5 interacts with various effector proteins, including adaptor protein complexes (AP-1 and AP-3), COPI, and phospholipase D, to mediate its diverse cellular functions.
Yes, understanding the role of ARF5 in disease processes may lead to the development of targeted therapies aimed at modulating its activity, thereby affecting specific cellular functions and pathways. However, further research is needed to establish its clinical relevance.
ARF5 activity is regulated by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), which control the loading and hydrolysis of GTP on ARF5, respectively. These regulators modulate ARF5's activation state and its interaction with downstream effectors.
The potential role of ARF5 in cancer is still being explored. It has been implicated in tumor progression and metastasis. Targeting ARF5 activity or its downstream effectors may hold promise for therapeutic strategies, but further research is needed to determine its clinical significance.
ARF5 has been shown to play a role in immune cell function. It is involved in processes such as phagocytosis, cytokine secretion, and immune cell migration. ARF5 regulates vesicle trafficking events that are crucial for immune cell activation and response.
Yes, ARF5 can be regulated by post-translational modifications. It can undergo phosphorylation, acetylation, and ubiquitination, among other modifications. These modifications can affect ARF5's activity, localization, and interactions with other proteins.
Currently, there are no known drugs or compounds specifically designed to target ARF5 protein. However, with ongoing research and understanding of its functions and implications in diseases, future therapeutic interventions may emerge.
Yes, ARF5 can interact with various proteins involved in endocytosis and vesicle trafficking. It interacts with clathrin, adaptors, and other coat proteins to facilitate the formation and maturation of clathrin-coated vesicles. ARF5 also interacts with effector proteins involved in membrane trafficking, such as GEFs and GAPs.
Yes, ARF5 protein can be regulated by cellular signaling pathways. Activation of various signaling pathways, such as G protein-coupled receptor (GPCR) signaling or growth factor signaling, can affect ARF5 activity through the modulation of its GEFs and GAPs.
Yes, ARF5 has been shown to play a role in membrane trafficking of neurotransmitters in neurons. It is involved in the regulation of synaptic vesicle dynamics and neurotransmitter release. It helps in the docking and fusion of synaptic vesicles with the plasma membrane during exocytosis.
es, ARF5 is expressed in a wide range of tissues and cell types, indicating its importance in fundamental cellular processes.
ARF5 has been implicated in cancer metastasis and tumor progression. It can regulate processes such as cell migration, invasion, and cytoskeletal remodeling, which are involved in cancer metastasis. Dysregulation of ARF5 activity may contribute to the invasive behavior of cancer cells.
Yes, mutations in the ARF5 gene have been identified in individuals with developmental disorders, such as intellectual disability and autism spectrum disorders. These mutations may affect ARF5 function and disrupt cellular processes involved in neuronal development and function.
Targeting ARF5 protein could have therapeutic potential in diseases involving dysregulated membrane trafficking or cellular processes that ARF5 is involved in. However, further research is needed to fully understand the mechanisms and consequences of modulating ARF5 activity before it can be considered as a specific therapeutic target.
Yes, ARF5 can undergo post-translational modifications such as phosphorylation, acetylation, and lipid modifications, which can modulate its activity, localization, and interaction with effector proteins.
Yes, ARF5 can interact with various proteins, such as ARFGAP1, ARFGAP3, and ARFIP2, to regulate its localization, activation, and downstream signaling.
Customer Reviews (8)
Write a reviewTheir commitment to providing technical support is unparalleled, and their expertise can prove invaluable in navigating any challenges or troubleshooting that may arise during my experiments.
the ARF5 Protein's applicability in protein electron microscopy structure analysis is remarkable.
Their extensive knowledge and expertise can help troubleshoot issues or provide guidance on experimental design, enabling you to overcome obstacles and achieve your research goals.
Its purity and functional integrity guarantee optimal performance in a wide range of experimental applications.
This protein, manufactured with utmost precision, provides reliable and consistent results, ensuring the accuracy and reproducibility of your research findings.
This commitment to staying at the forefront of research translates directly to the quality and performance of the ARF5 protein in my trials.
They monitor the latest advancements in the field and incorporate new findings into the production and characterization of the protein.
the ARF5 protein's versatility allows it to be effectively employed in various experimental designs, further enhancing its suitability for diverse research needs.
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