Recombinant Human ARIH1 protein, GST-tagged

• Cat.No.: ARIH1-799H
• Product Overview: Human ARIH1 full-length ORF (BAG35590.1, 1 a.a. - 557 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: ARIH1 (Ariadne RBR E3 Ubiquitin Protein Ligase 1) is a Protein Coding gene. Diseases associated with ARIH1 include Sleep Disorder and Aortic Valve Insufficiency. Among its related pathways are Interferon gamma signaling and Immune System. GO annotations related to this gene include ligase activity and ubiquitin protein ligase binding. An important paralog of this gene is ARIH2.
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 87.67 kDa
• AA Sequence: MDSDEGYNYEFDEDEECSEEDSGAE EEEDEDDDEPDDDTLDLGEVELVEP GLGVGGERDGLLCGETGGGGGSALG PGGGGGGGGGGGGGGPGHEQEEDYR YEVLTAEQILQHMVECIREVNEVIQ NPATITRILLSHFNWDKEKLMERYF DGNLEKLFAECHVINPSKKSRTRQM NTRSSAQDMPCQICYLNYPNSYFTG LECGHKFCMQCWSEYLTTKIMEEGM GQTISCPAHGCDILVDDNTVMRLIT DSKVKLKYQHLITNSFVECNRLLKW CPAPDCHHVVKVQYPDAKPVRCKCG RQFCFNCGENWHDPVKCKWLKKWIK KCDDDSETSNWIAANTKECPKCHVT IEKDGGCNHMVCRNQNCKAEFCWVC LGPWEPHGSAWYNCNRYNEDDAKAA RDAQERSRAALQRYLFYCNRYMNHM QSLRFEHKLYAQVKQKMEEMQQHNM SWIEVQFLKKAVDVLCQCRATLMYT YVFAFYLKKNNQSIIFENNQADLEN ATEVLSGYLERDISQDSLQDIKQKV QDKYRYCESRRRVLLQHVHEGYEKD LWEYIED
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ARIH1 ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila) [ Homo sapiens ]
• Official Symbol: ARIH1
• Synonyms: ARIH1; ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila); ariadne (Drosophila) homolog, ubiquitin conjugating enzyme E2 binding protein, 1; E3 ubiquitin-protein ligase ARIH1; ARI; ariadne; Drosophila; homolog of; HARI; HHARI; UBCH7BP; ARI-1; MOP-6; H7-AP2; monocyte protein 6; ubcH7-binding protein; protein ariadne-1 homolog; ubcM4-interacting protein; ariadne, Drosophila, homolog of; ubiquitin-conjugating enzyme E2-binding protein 1; FLJ20329; FLJ93118; DKFZp686O13120
• Gene ID: 25820
• mRNA Refseq: NM_005744
• Protein Refseq: NP_005735
• MIM: 605624
• UniProt ID: Q9Y4X5

Reviews

11/11/2022

Extensive research has been conducted on ARIH1, making it relatively accessible and well-understood in terms of its structure and functions.

05/18/2022

The manufacturer's excellent technical support, commitment to innovation, and customer-centric approach further reinforce its suitability for my research.

02/12/2022

The manufacturer's commitment to providing exceptional technical support ensures that I will receive valuable assistance when facing challenges in my research.

12/06/2020

They actively engage with researchers, comprehending their specific experimental needs, and offering tailored services accordingly.

08/21/2020

Their knowledgeable team is readily available to address any concerns or difficulties I may encounter during my experiments. With their expertise, I can confidently overcome any obstacles and achieve optimal results.

07/04/2019

the ARIH1 protein offers superior quality and reliability, making it a perfect fit for my experimental needs.

12/11/2018

With their support, I can confidently explore the intricacies of ARIH1 and make significant contributions to the understanding of its functions in various biological processes.

07/04/2017

Its versatility allows for its effective utilization in a broad range of studies, including molecular biology, cellular assays, and protein interactions analysis.

Q&As

Are there any known functional domains or motifs within the ARIH1 protein structure?

Yes, the ARIH1 protein contains several functional domains, including an N-terminal RBR (RING-between-RING) domain, which is responsible for its E3 ubiquitin ligase activity. It also has a central coiled-coil domain and a C-terminal region that contributes to its protein-protein interactions. These domains collectively contribute to the diverse functions of the ARIH1 protein.

Are there any known interacting proteins or cofactors of the ARIH1 protein?

Yes, the ARIH1 protein interacts with various proteins and cofactors to carry out its functions. Some known interacting proteins include BRCA1, TRAF6, and UbcH7. These interactions play a role in modulating ARIH1 activity and its involvement in different cellular pathways.

How is the ARIH1 protein involved in innate immune signaling?

The ARIH1 protein is known to play a role in innate immune signaling by regulating the activation of the NF-κB pathway. It interacts with and promotes the ubiquitination and degradation of proteins involved in NF-κB signaling, thereby controlling the immune response.

Are there any diseases associated with ARIH1 dysfunction?

Yes, mutations in the ARIH1 gene have been associated with a rare autosomal recessive disorder known as autoinflammation with arthritis and dyskeratosis (AIADK). This condition is characterized by recurrent fever episodes, arthritis, and other systemic symptoms.

Does the ARIH1 protein have any role in neurodegenerative diseases?

The involvement of the ARIH1 protein in neurodegenerative diseases is still an area of ongoing research. Some studies suggest that dysregulation of ARIH1 may contribute to the pathogenesis of neurodegenerative diseases, such as Parkinson's disease. However, more research is needed to fully understand its role in these conditions.

Are there any drugs targeting the ARIH1 protein?

Currently, there are no specific drugs that directly target the ARIH1 protein. However, understanding its role in disease pathways may help identify potential therapeutic strategies indirectly targeting ARIH1-associated conditions.

Are there any cellular pathways or molecular processes where the ARIH1 protein is still being studied?

The ARIH1 protein is actively being studied in several areas of research. Some ongoing areas of investigation include its role in cancer development and progression, its involvement in cellular stress responses, and its impact on protein homeostasis. Further research is needed to fully understand its contributions in these contexts.

Does the ARIH1 protein have any role in mitochondrial quality control?

Yes, the ARIH1 protein has been found to be involved in mitochondrial quality control. It helps in the removal of damaged or dysfunctional mitochondria through a process called mitophagy, ensuring the maintenance of cellular homeostasis.

How is the ARIH1 protein linked to autoinflammation with arthritis and dyskeratosis (AIADK)?

Mutations in the ARIH1 gene have been associated with AIADK, a rare autosomal recessive disorder. These mutations lead to the loss of ARIH1 function, resulting in dysregulation of immune responses and inflammatory manifestations seen in AIADK patients.

Is the ARIH1 protein involved in DNA damage repair?

Yes, studies suggest that the ARIH1 protein is involved in DNA damage repair pathways. It interacts with other proteins involved in DNA repair, such as BRCA1, and contributes to the maintenance of genomic stability.

Can ARIH1 gene mutations be inherited and passed on to future generations?

Yes, mutations in the ARIH1 gene can be inherited in an autosomal recessive manner. This means that an individual must inherit two copies of the mutated gene, one from each parent, in order to develop the associated disorder or condition.

How does the ARIH1 protein contribute to protein degradation?

ARIH1 functions as an E3 ubiquitin ligase, which means it catalyzes the transfer of ubiquitin molecules onto target proteins. This process marks the proteins for degradation by the proteasome, a cellular recycling machinery.