Recombinant Human ARL4C 293 Cell Lysate

• Cat.No.: ARL4C-8712HCL

Specification

• Description: Antigen standard for ADP-ribosylation factor-like 4C (ARL4C) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ARL4C ADP-ribosylation factor-like 4C [ Homo sapiens ]
• Official Symbol: ARL4C
• Synonyms: LAK; ARL7
• Gene ID: 10123
• mRNA Refseq: NM_005737.3
• Protein Refseq: NP_005728.2
• MIM: 604787
• UniProt ID: P56559
• Chromosome Location: 2q37.1
• Function: GTP binding;GTPase activity;alpha-tubulin binding;protein binding

Reviews

02/12/2023

What sets ARL4C apart is the manufacturer's unparalleled technical support.

03/07/2021

With the ARL4C protein and the manufacturer's support, I can confidently pursue my research goals and achieve meaningful outcomes.

05/17/2019

This adaptability ensures that the ARL4C protein can effectively address my specific research objectives, enabling breakthrough discoveries.

02/17/2018

The ARL4C protein is an outstanding product that possesses exceptional quality, making it an ideal choice for my experimental needs.

12/20/2017

The manufacturer's commitment to delivering excellent technical support further enhances the experience, offering timely resolutions to any challenges I may encounter.

05/18/2017

the ARL4C protein surpasses expectations in terms of quality, meeting the rigorous demands of my experiments.

04/24/2017

Their team of experts is readily available to address any issues that may arise, providing solutions to problems and guiding me through the experimental process.

03/27/2017

Its seamless integration makes it possible to explore different research avenues, expanding the scope and impact of my investigations.

03/08/2016

Its purity and consistency have been extensively validated, minimizing any potential variability that could affect trial outcomes.

02/28/2016

With the manufacturer's excellent technical support and its wide range of applications, the ARL4C protein has become an indispensable tool, aiding in my research endeavors and driving meaningful scientific discoveries.

Q&As

Can the ARL4C protein be targeted for therapeutic purposes?

The therapeutic potential of targeting ARL4C is currently being explored. Its involvement in cancer and potential as a biomarker make it a promising target for therapeutic interventions. However, further research is necessary to fully understand its mechanisms and develop targeted therapies.

Are there any known disease associations or mutations in the ARL4C gene?

Mutations in the ARL4C gene have been associated with certain types of cancer, including melanoma and hepatocellular carcinoma. Dysregulation of ARL4C expression or activity may contribute to disease progression and metastasis.

Is ARL4C involved in any signaling pathways?

ARL4C is not considered a direct signaling molecule in canonical signaling pathways. However, it may indirectly influence the activity of certain signaling pathways through its role in membrane dynamics and protein transport.

Can dysregulation of ARL4C expression lead to any cellular defects?

Dysregulation of ARL4C expression or activity can potentially lead to defects in vesicular trafficking, cellular structure organization, and overall cellular homeostasis. Such defects may contribute to disease development and cellular dysfunction.

In which tissues or cell types is ARL4C expressed?

ARL4C is found to be expressed in various tissues and cell types, indicating its widespread presence. Its expression may differ depending on the specific cellular context.

Does ARL4C interact with other proteins?

Yes, ARL4C interacts with various proteins involved in vesicle trafficking, cytoskeletal dynamics, and intracellular signaling. It can interact with GTPase-activating proteins (GAPs), guanine nucleotide exchange factors (GEFs), and effector proteins to modulate cellular processes.