Recombinant Human ARL6 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ARL6-622H
• Product Overview: ARL6 MS Standard C13 and N15-labeled recombinant protein (NP_816931) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene belongs to the ARF-like (ADP ribosylation factor-like) sub-family of the ARF family of GTP-binding proteins which are involved in regulation of intracellular traffic. Mutations in this gene are associated with Bardet-Biedl syndrome (BBS). A vision-specific transcript, encoding long isoform BBS3L, has been described (PMID: 20333246).
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 21.1 kDa
• AA Sequence: MGLLDRLSVLLGLKKKEVHVLCLGL DNSGKTTIINKLKPSNAQSQNILPT IGFSIEKFKSSSLSFTVFDMSGQGR YRNLWEHYYKEGQAIIFVIDSSDRL RMVVAKEELDTLLNHPDIKHRRIPI LFFANKMDLRDAVTSVKVSQLLCLE NIKDKPWHICASDAIKGEGLQEGVD WLQDQIQTVKTTRTRPLEQKLISEE DLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ARL6 ADP-ribosylation factor-like 6 [ Homo sapiens (human) ]
• Official Symbol: ARL6
• Synonyms: ARL6; ADP ribosylation factor like GTPase 6; BBS3; RP55; ADP-ribosylation factor-like protein 6; Bardet-Biedl syndrome 3 protein
• Gene ID: 84100
• mRNA Refseq: NM_177976
• Protein Refseq: NP_816931
• MIM: 608845
• UniProt ID: Q9H0F7

Reviews

03/03/2023

the ARL6 protein's quality and suitability for experimental needs, combined with the manufacturer's excellent technical support, create a valuable partnership that enhances research outcomes.

02/07/2021

Researchers can feel confident in the reliability and efficacy of the ARL6 protein and rely on the manufacturer's expertise to navigate any challenges that may arise during their trials.

12/14/2019

This may include options like different formulations, protein modifications, or tagging techniques. Customization can enhance the versatility of the protein and optimize its utility in the intended research.

10/27/2019

They can also address any questions or concerns regarding the ARL6 protein, thereby facilitating smooth and efficient research execution.

09/24/2019

manufacturers may offer customization options, allowing researchers to tailor the ARL6 protein to their specific needs.

10/15/2017

the manufacturer's technical support team can offer prompt assistance in case any issues arise during the handling or application of the ARL6 protein.

07/04/2016

Such support ensures researchers have access to reliable tools and expertise, facilitating their investigations into the functions, mechanisms, and disease relevance of ARL6 protein.

06/10/2016

The ARL6 protein is a valuable resource for researchers due to its high quality and its ability to meet specific experimental needs.

Q&As

Does ARL6 have any functional implications in diseases other than ciliopathies?

While ARL6's primary role is associated with cilia and ciliopathies, emerging evidence suggests its involvement in other diseases. ARL6 dysregulation has been associated with cancer progression and metastasis in certain contexts, highlighting its potential in disease beyond ciliopathies.

Are there any known post-translational modifications of ARL6?

Yes, ARL6 can undergo post-translational modifications such as prenylation, which involves the addition of a lipid moiety to the protein. Prenylation is essential for targeting ARL6 to cellular membranes and for its proper functioning in ciliary processes.

What are the known interacting partners of ARL6?

ARL6 interacts with various proteins involved in vesicular trafficking and ciliary processes. Some known interacting partners include BBS1, BBS2, BBS4, BBS5, and BBS7, which are components of the BBSome complex involved in ciliogenesis. ARL6 also interacts with Rab proteins, such as Rab8, Rab11, and Rab11-family interacting proteins (FIPs), which play crucial roles in vesicle trafficking and ciliogenesis.

Is ARL6 protein conserved across species?

Yes, ARL6 is highly conserved across various species, including mammals, fish, worms, and flies. This conservation suggests its essential role in fundamental cellular processes and highlights its functional significance.

Are there any known regulatory mechanisms for ARL6 expression?

The regulatory mechanisms controlling ARL6 expression are still being investigated. However, studies have suggested that transcription factors, regulatory elements in the ARL6 gene promoter region, and epigenetic modifications can influence ARL6 expression levels. Further research is required to fully understand the precise regulatory mechanisms governing ARL6 expression.

Is ARL6 associated with any other cellular processes apart from ciliary trafficking?

While ARL6's primary role is in ciliary trafficking, emerging evidence suggests its involvement in other cellular processes. ARL6 has been implicated in Golgi organization and Secretory Carrier Membrane Proteins (SCAMPs) trafficking. Additionally, ARL6 has been linked to intracellular calcium signaling and cell growth regulation.

Is the expression of ARL6 tissue-specific?

ARL6 is widely expressed in various tissues and cell types. It is present in most mammalian tissues, including the brain, kidney, liver, and reproductive organs. However, its expression may vary in different cell types and developmental stages.

Can ARL6 be targeted for therapeutic intervention?

Targeting ARL6 directly for therapeutic intervention is challenging. However, understanding its role and interactions can provide insights into developing treatments for ciliopathies like BBS. Modulating downstream effectors or pathways regulated by ARL6 may hold potential for therapeutic strategies.

Are there any studies investigating the potential therapeutic interventions for ARL6-related diseases?

Currently, specific therapeutic interventions targeting ARL6 are not available. However, studies are ongoing to identify potential therapeutic strategies indirectly targeting ARL6-associated pathways, such as modulating cilia biology or vesicular trafficking processes. Additionally, research aims to understand the underlying molecular mechanisms of ARL6-related diseases, which can help in developing targeted therapies in the future.

Are there any known interacting partners of ARL6?

ARL6 interacts with multiple proteins involved in cilia biogenesis, including BBSome proteins like BBS1, BBS4, and BBS8. It also interacts with other cilia-related proteins like IFT-B proteins (part of the intraflagellar transport machinery) and Rab-like GTPases. These interactions are critical for proper cilia assembly and function.

Can ARL6 expression levels be used as a diagnostic marker for ciliopathies?

ARL6 expression levels alone may not serve as a specific diagnostic marker for ciliopathies like BBS, as other genetic and environmental factors can contribute to the development of these disorders. However, evaluating ARL6 expression in conjunction with other clinical and genetic indicators may aid in the diagnosis and characterization of ciliopathies.

What are the phenotypic consequences of ARL6 mutations?

Mutations in ARL6 are associated with several ciliopathies, including Bardet-Biedl syndrome (BBS) and primary ciliary dyskinesia (PCD). These conditions are characterized by a range of phenotypes, including obesity, retinal degeneration, renal abnormalities, polydactyly, and respiratory problems.

How does ARL6 contribute to ciliary processes?

ARL6 is involved in the formation and maintenance of primary cilia, which are sensory organelles found on the surface of many cells. It is essential for ciliary cargo trafficking and regulates the transport of proteins, vesicles, and signaling molecules in and out of the cilium. ARL6 also plays a role in cilia length regulation and ciliary membrane dynamics.

Can ARL6 be targeted pharmacologically for therapeutic purposes?

Currently, there are no specific drugs targeting ARL6. However, understanding its role in various diseases can pave the way for the development of therapeutic strategies indirectly targeting ARL6-associated pathways, such as modulating cilia function or vesicular trafficking processes.

Are there any animal models available to study ARL6-related conditions?

Yes, animal models such as mouse and zebrafish models have been generated with ARL6 mutations or knockouts to study the impact on cilia formation and associated diseases like BBS. These models provide valuable insights into the role of ARL6 in vivo and facilitate the discovery of potential therapeutic interventions.

How does ARL6 contribute to ciliogenesis?

ARL6 is a key regulator of ciliogenesis, the process of cilia formation. It interacts with other proteins in the cilia assembly machinery and participates in the trafficking of proteins required for cilia formation and maintenance. ARL6 mutations can impair cilia assembly and function, leading to ciliopathies like BBS.

Is ARL6 associated with any other cellular processes other than ciliogenesis?

Besides its role in ciliogenesis, ARL6 has been implicated in other cellular processes such as endosomal trafficking, autophagy, and membrane dynamics. It interacts with proteins involved in these processes, suggesting additional functions beyond cilia regulation.

Are there any ongoing research efforts targeting ARL6?

Research on ARL6 is ongoing, primarily focusing on understanding its precise role in ciliary processes, identifying novel interacting partners, exploring its involvement in diseases beyond ciliopathies, and investigating potential therapeutic approaches. Ongoing studies aim to elucidate the molecular mechanisms underlying ARL6 function and its broader implications in cellular physiology and pathology.

How is ARL6 involved in vesicular trafficking?

ARL6 regulates vesicular trafficking by interacting with various proteins involved in this process, such as Rab proteins and SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins. ARL6 modulates the fusion of vesicles with target membranes and ensures proper cargo delivery.

Can ARL6 mutations cause any genetic disorders?

Yes, mutations in the ARL6 gene have been associated with several genetic disorders, including Bardet-Biedl syndrome (BBS), which is characterized by obesity, retinal degeneration, polydactyly, kidney abnormalities, and learning disabilities. ARL6 mutations can disrupt cilia function, leading to the development of BBS and other related disorders.