Recombinant Human ARPC2 protein, GST-tagged

• Cat.No.: ARPC2-1207H
• Product Overview: Recombinant Human ARPC2 protein(1-300 aa), fused to GST tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: GST
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8.). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 100mM GSH.
• Protein length: 1-300 aa
• AA Sequence: MILLEVNNRIIEETLALKFENAAAG NKPEAVEVTFADFDGVLYHISNPNG DKTKVMVSISLKFYKELQAHGADEL LKRVYGSFLVNPESGYNVSLLYDLE NLPASKDSIVHQAGMLKRNCFASVF EKYFQFQEEGKEGENRAVIHYRDDE TMYVESKKDRVTVVFSTVFKDDDDV VIGKVFMQEFKEGRRASHTAPQVLF SHREPPLELKDTDAAVGDNIGYITF VLFPRHTNASARDNTINLIHTFRDY LHYHIKCSKAYIHTRMRAKTSDFLK VLNRARPDAEKKEMKTITGKTFSSR
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: ARPC2 actin related protein 2/3 complex, subunit 2, 34kDa [ Homo sapiens ]
• Official Symbol: ARPC2
• Synonyms: ARPC2; actin related protein 2/3 complex, subunit 2, 34kDa; actin related protein 2/3 complex, subunit 2 (34 kD); actin-related protein 2/3 complex subunit 2; ARC34; p34 Arc; arp2/3 complex 34 kDa subunit; ARP2/3 protein complex subunit 34; PRO2446; p34-Arc; PNAS-139
• Gene ID: 10109
• mRNA Refseq: NM_005731
• Protein Refseq: NP_005722
• MIM: 604224
• UniProt ID: O15144

Reviews

01/23/2023

Its remarkable sensitivity and specificity make it a valuable tool for accurate and robust data analysis, bolstering the credibility of research outcomes.

08/28/2018

Notably, it exhibits exceptional performance in ELISA assays, providing reliable and precise results in the detection and quantification of target molecules.

10/30/2016

Highly recommended!

Q&As

What cellular processes rely on ARPC2 and the Arp2/3 complex?

The Arp2/3 complex, including ARPC2, is essential for various cellular processes. It plays a critical role in cell migration and adhesion, enabling cells to move and change shape. The complex is also involved in endocytosis, phagocytosis, and vesicle trafficking, regulating membrane dynamics. Additionally, ARPC2 and the Arp2/3 complex contribute to cell division, neuronal development, and immune cell function.

How is the expression of ARPC2 regulated at the molecular level?

The expression of ARPC2 can be regulated transcriptionally through the binding of specific transcription factors to the promoter region of the ARPC2 gene. Additionally, post-transcriptional mechanisms like microRNAs or RNA-binding proteins can also affect the stability or translation efficiency of ARPC2 mRNA. Furthermore, epigenetic modifications, such as DNA methylation or histone acetylation, can play a role in regulating ARPC2 gene expression.

Are there any known diseases or disorders associated with ARPC2 mutations?

Mutations in the ARPC2 gene have been linked to a rare genetic disorder called Wiskott-Aldrich syndrome (WAS). WAS is characterized by immune system abnormalities, eczema, and a higher risk of developing certain types of cancer. Although mutations in the ARPC2 gene are relatively rare in WAS, they can disrupt the normal function of the Arp2/3 complex, leading to actin cytoskeleton defects.

Is the expression of the ARPC2 gene regulated during development?

Yes, the expression of the ARPC2 gene can be regulated during development. Transcription factors specific to certain developmental stages or tissues can bind to the regulatory regions of the gene and control its expression. Additionally, epigenetic modifications may also influence the expression patterns of ARPC2 during development.

Are there any known inhibitors or activators of ARPC2?

Currently, there are no specific inhibitors or activators targeting ARPC2 directly. However, various compounds and drugs that target the Arp2/3 complex or actin cytoskeleton dynamics in general can indirectly impact the function of ARPC2. These include small molecule inhibitors of actin polymerization and drugs that modulate actin-binding proteins involved in regulating ARPC2 activity.

Are there any interacting proteins or complexes that interact with ARPC2?

ARPC2 interacts with several other proteins and complexes involved in actin cytoskeleton regulation. It forms a stable complex with other subunits (ARPC1-5) to constitute the Arp2/3 complex. This complex interacts with nucleation promoting factors (NPFs) such as WASP and WAVE, which facilitate actin filament branching. Furthermore, ARPC2 can also interact with actin-binding proteins and signaling molecules, leading to cytoskeletal rearrangements and cellular responses.