Recombinant Human ARPC3, GST-tagged

• Cat.No.: ARPC3-9884H
• Product Overview: Recombinant Human ARPC3 protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Description: This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p21 subunit, has yet to be determined.
• Source: E.coli
• Species: Human
• Tag: GST
• Protein length: 1-178a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.

Gene Information

• Gene Name: ARPC3 actin related protein 2/3 complex, subunit 3, 21kDa [ Homo sapiens ]
• Official Symbol: ARPC3
• Synonyms: ARPC3; actin related protein 2/3 complex, subunit 3, 21kDa; actin related protein 2/3 complex, subunit 3 (21 kD); actin-related protein 2/3 complex subunit 3; ARC21; p21 Arc; arp2/3 complex 21 kDa subunit; ARP2/3 protein complex subunit p21; p21-Arc
• Gene ID: 10094
• mRNA Refseq: NM_005719
• Protein Refseq: NP_005710
• MIM: 604225
• UniProt ID: O15145
• Chromosome Location: 12q24
• Pathway: B Cell Receptor Signaling Pathway, organism-specific biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem; CDC42 signaling events, organism-specific biosystem; ErbB1 downstream signaling, organism-specific biosystem; Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem
• Function: actin binding; protein binding; structural constituent of cytoskeleton

Reviews

04/04/2021

the ARPC3 protein finds great utility in protein electron microscopy structure analysis, consistently delivering exceptional results.

11/15/2020

Its stability and functional attributes ensure reliable imaging and facilitate the elucidation of complex protein structures.

02/01/2020

Its versatility and reliability make it an indispensable asset for scientists seeking to explore the complexities of various biological systems.

Q&As

Is the ARPC3 protein essential for cell viability?

Yes, the ARPC3 protein is essential for cell viability as it is a core component of the ARP2/3 complex, which is crucial for actin polymerization and cytoskeletal organization.

How is the ARPC3 protein involved in cellular processes?

The ARPC3 protein is involved in a wide range of cellular processes that require actin dynamics, such as cell motility, endocytosis, cell division, and formation of cellular protrusions like lamellipodia and filopodia.

How can the ARPC3 protein be regulated?

The ARPC3 protein can be regulated through various mechanisms. Factors such as phosphorylation, protein-protein interactions, and post-translational modifications can modulate its activity and influence actin dynamics in cells.

Can targeting the ARPC3 protein have therapeutic implications?

Targeting the ARPC3 protein or the ARP2/3 complex could have potential therapeutic implications for diseases characterized by dysregulated actin dynamics, such as cancer metastasis, autoimmune disorders, and neurodegenerative diseases. Developing specific inhibitors or modulators of ARPC3 activity could be explored as potential therapeutic strategies.

Are there any known diseases associated with alterations in ARPC3 expression or function?

Mutations or dysregulation of the ARPC3 gene have been linked to developmental disorders and genetic syndromes, including Baraitser-Winter syndrome and distal arthrogryposis.

Are there any known binding partners of the ARPC3 protein?

The ARPC3 protein interacts with other components of the ARP2/3 complex, such as ARPC1, ARPC2, ARPC4, and ARPC5, to form a functional actin nucleation complex. It also interacts with various actin-binding proteins and regulators to mediate actin dynamics.

How can researchers study the ARPC3 protein in the laboratory?

Researchers can study the ARPC3 protein by using techniques such as immunoblotting, immunofluorescence microscopy, co-immunoprecipitation, and gene knockout or knockdown approaches in cell culture models to assess its expression, localization, interactions, and functional effects.