Recombinant Human ART1 Protein (Met1-Ser295), C-His tagged

• Cat.No.: ART1-4516H
• Product Overview: Recombinant human ART1 (Met1-Ser295) fused with the C-terminal His tag was expressed in Mammalian cells.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: C-His
• Form: Lyophilized powder/frozen liquid
• Molecular Mass: 32.45 kDa
• Protein Length: Met1-Ser295
• Endotoxin: < 1.0 EU/μg of the protein as determined by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 centigrade for one week. Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: Supplied as solution form in PBS or lyophilized from PBS.
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Gene Name: ART1 ADP-ribosyltransferase 1 [ Homo sapiens (human) ]
• Official Symbol: ART1
• Synonyms: ART1; ADP-ribosyltransferase 1; GPI-linked NAD (P) (+) --arginine ADP-ribosyltransferase 1; ART2; CD296; ADP-ribosyltransferase 2; mono (ADP-ribosyl) transferase 1; RT6; MGC133217
• Gene ID: 417
• mRNA Refseq: NM_004314
• Protein Refseq: NP_004305
• MIM: 601625
• UniProt ID: P52961

Reviews

01/16/2022

One aspect that sets the ART1 protein apart is the excellent technical support provided by the manufacturer.

01/18/2020

Its purity and reliability have consistently resulted in successful outcomes in my research projects, providing me with accurate and reproducible data.

03/23/2016

The ART1 protein has demonstrated remarkable stability, allowing for extended storage without compromising its quality.

Q&As

How does ART1 carry out ADP-ribosylation?

ART1 transfers ADP-ribose from nicotinamide adenine dinucleotide (NAD+) to specific arginine residues on substrate proteins, modifying their structure and function.

Are there any known substrates that ART1 acts upon?

ART1 predominantly ADP-ribosylates arginine residues on G protein-coupled receptors (GPCRs), such as the P2Y purinergic receptor.

Are there any known natural inhibitors or regulators of ART1 activity?

Some pathogens produce enzymes called ADP-ribosyltransferase toxins that can inhibit ART1 activity and evade the immune response.

In which tissues or organs is ART1 predominantly expressed?

ART1 is primarily expressed in the testes, but it can also be found in other tissues such as the spleen, kidney, and liver.

Does ART1 have any non-enzymatic functions or interactions with other proteins?

Aside from its enzymatic role in ADP-ribosylation, ART1 does not have any known non-enzymatic functions or specific protein interactions.

Are there any known diseases or disorders associated with mutations in the ART1 gene?

Mutations in the ART1 gene have not been directly linked to specific diseases or disorders in humans so far.

Is the activity of ART1 influenced by any cofactors or regulators?

The activity of ART1 is dependent on the presence of NAD+ as a substrate, and it can be modulated by other factors, including pH and the presence of certain ions.